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Summary

Bio::Align::PairwiseStatistics - Base statistic object for Pairwise Alignments

Package variables

Inherit

Bio::Align::StatisticsI Bio::Root::Root

Synopsis

  use strict;
  my $stats = Bio::Align::PairwiseStatistics->new();

  # get alignment object of two sequences somehow
  my $pwaln;
  print $stats->number_of_comparable_bases($pwaln);
  my $score = $stats->score_nuc($pwaln);

Description

Calculate pairwise statistics.

Methods

Methods description

 Title   : number_of_comparable_bases
 Usage   : my $bases = $stat->number_of_comparable_bases($aln);
 Function: Returns the count of the number of bases that can be
           compared (L) in this alignment ( length - gaps)
 Returns : integer
 Args    : Bio::Align::AlignI

 Title   : number_of_differences
 Usage   : my $nd = $stat->number_of_distances($aln);
 Function: Returns the number of differences between two sequences
 Returns : integer
 Args    : Bio::Align::AlignI

 Title   : number_of_gaps
 Usage   : my $nd = $stat->number_of_gaps($aln);
 Function: Returns the number of gapped positions among sequences in alignment
 Returns : integer
 Args    : Bio::Align::AlignI

 Title   : score_nuc
 Usage   : my $score = $stat->score_nuc($aln);
             or
           my $score = $stat->score_nuc(
             -aln =>$aln,
             -match    => 1,
             -mismatch => -1,
             -gap_open => -1,
             -gap_ext  => -1
           );
 Function: Calculate the score of an alignment of 2 nucleic acid sequences. The
           scoring parameters can be specified. Otherwise the blastn default
           parameters are used: match = 2, mismatch = -3, gap opening = -5, gap
           extension = -2
 Returns : alignment score (number)
 Args    : Bio::Align::AlignI
           match score [optional]
           mismatch score [optional]
           gap opening score [optional]
           gap extension score [optional]

Methods code

BEGIN {

 $GapChars = '(\.|\-)';

}

sub number_of_comparable_bases {

   my ($self,$aln) = @_;
  if ( ! defined $aln || ! $aln->isa('Bio::Align::AlignI') ) {
    $self->throw("Must provide a Bio::Align::AlignI compliant object to ".
      "Bio::Align::PairwiseStatistics");
       return 0;
  } elsif ( $aln->num_sequences != 2 ) { 
    $self->throw("Only pairwise calculations supported. Found ".
      $aln->num_sequences." sequences in alignment\n");
   }
   my $L = $aln->length - $self->number_of_gaps($aln);
   return $L;

}

sub number_of_differences {

   my ($self,$aln) = @_;
    if( ! defined $aln || ! $aln->isa('Bio::Align::AlignI') ) {
    $self->throw("Must provide a Bio::Align::AlignI compliant object to ".
      "Bio::Align::PairwiseStatistics");
  } elsif ( $aln->num_sequences != 2 ) { 
    $self->throw("Only pairwise calculations supported. Found ".
      $aln->num_sequences." sequences in alignment\n");
    }
   my (@seqs);
  foreach my $seq ( $aln->each_seq ) {
       push @seqs, [ split(//,$seq->seq())];
   }
   my $firstseq = shift @seqs;
  #my $secondseq = shift @seqs;
   my $diffcount = 0;
   for (my $i = 0;$i<$aln->length; $i++ ) {
    next if ( $firstseq->[$i]  =~ /^$GapChars$/ );
       foreach my $seq ( @seqs ) {
      next if ( $seq->[$i]  =~ /^$GapChars$/ );
	   if( $firstseq->[$i] ne $seq->[$i] ) {
	       $diffcount++;
	   }
       }
   }
   return $diffcount;

}

sub number_of_gaps {

   my ($self,$aln) = @_;
  if ( ! defined $aln || ! $aln->isa('Bio::Align::AlignI') ) {
    $self->throw("Must provide a Bio::Align::AlignI compliant object to ".
      "Bio::Align::PairwiseStatistics");
  } elsif ( $aln->num_sequences != 2 ) { 
    $self->throw("Only pairwise calculations supported. Found ".
      $aln->num_sequences." sequences in alignment\n");
    }
   my $gapline = $aln->gap_line;
   # this will count the number of '-' characters
   return $gapline =~ tr/-/-/;

}

sub score_nuc {

  my ($self, @args) = @_;
  my ( $aln, $match, $mismatch, $gap_open, $gap_ext) = $self->_rearrange( [qw(
    ALN MATCH MISMATCH GAP_OPEN GAP_EXT)], @args );
  if ( ! defined $aln || ! $aln->isa('Bio::Align::AlignI') ) {
    $self->throw("Must provide a Bio::Align::AlignI compliant object to ".
      "Bio::Align::PairwiseStatistics");
  } elsif ( $aln->num_sequences != 2 ) { 
    $self->throw("Only pairwise calculations supported. Found ".
      $aln->num_sequences." sequences in alignment\n");
  }
  my $seq1 = $aln->get_seq_by_pos(1);
  my $seq2 = $aln->get_seq_by_pos(2);
  if (! ( ($seq1->alphabet eq 'dna' || $seq1->alphabet eq 'rna') &&
    ($seq2->alphabet eq 'dna' || $seq2->alphabet eq 'rna') )) {
    $self->throw("Can only score nucleic acid alignments");
  }
  $match    ||=  2; # Blastn scoring defaults
  $mismatch ||= -3;
  $gap_open ||= -5;
  $gap_ext  ||= -2;
  my $score = 0;
  my $prevres1 = '-';
  my $prevres2 = '-';
  for (my $pos = 1 ; $pos <= $aln->length ; $pos++) {
    my $res1 = $seq1->subseq($pos, $pos);
    my $res2 = $seq2->subseq($pos, $pos);
    if (!($res1 eq '-' || $res2 eq '-')) { # no gap
      if ($res1 eq $res2) { # same residue
        $score += $match;
      } else { # other residue
        $score += $mismatch;
      }
    } else { # open or ext gap?
      my $open = 0;
      if (!($res1 eq '-' && $res2 eq '-')) { # exactly one gap
        my $prevres = $prevres1;
        $prevres = $prevres2 if $res2 eq '-';
        $open = 1 unless $prevres eq '-';
      } else { # 2 gaps
        $open = 1 unless $prevres1 eq '-' && $prevres2 eq '-';
      }
      if ($open) {
        $score += $gap_open; # gap opening
      } else {
        $score += $gap_ext; # gap extension
      }
    }
    $prevres1 = $res1;
    $prevres2 = $res2;
  }
  return $score;
}

1;

}

General documentation

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The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _