| Summary | Included libraries | Package variables | Synopsis | Description | General documentation | Methods |
| WebCvs |
| _preferred_tags | No description | Code |
| new | Description | Code |
| segment | No description | Code |
| refclass | No description | Code |
| load_from_embl | No description | Code |
| load_from_file | No description | Code |
| _load_embl | No description | Code |
| get_attributes | No description | Code |
| guess_name | No description | Code |
| new | code | next | Top |
Title : newThis is the constructor for the adaptor. It is called automatically by Bio::DB::GFF->new. In addition to arguments that are common among all adaptors, the following class-specific arguments are recgonized: Argument Description-dsn,-user and -pass indicate the local database to cache results in, and as are per Bio::DB::GFF::Adaptor::dbi. The -proxy argument allows you to set the biofetch web proxy, and uses the same syntax described for the proxy() method of Bio::DB::WebDBSeqI, except that the argument must be passed as an array reference. |
| _preferred_tags | description | prev | next | Top |
my ($self, $tags) = @_; if ($tags && (ref($tags) =~ /HASH/)){ $self->{preferred_tags} = $tags; } return $self->{preferred_tags};}
| new | description | prev | next | Top |
my $class = shift; my $args = shift; my $self = $class->SUPER::new($args); my ($preferred) = rearrange(['PREFERRED_TAGS'],$args); $self->_preferred_tags($preferred?$preferred:\%default_preferred_tags); # if the caller sent their own preferences, then use these, otherwise use defaults.}
my ($proxy) = rearrange(['PROXY'],$args); if ($proxy) { my @args = ref($proxy) ? @$proxy : eval $proxy; $self->{_proxy} =\@ args if @args; } $self;
| segment | description | prev | next | Top |
my $self = shift; my @segments = $self->SUPER::segment(@_); if (!@segments) { my $refclass = $self->refclass; my %args = $self->setup_segment_args(@_); if ($args{-class} && $args{-class} =~ /$refclass/oi) { return unless $self->load_from_embl('embl'=>$args{-name}); @segments = $self->SUPER::segment(@_); } elsif ($args{-class} && $args{-class} =~ /refseq|swall|embl/i) { #hack to get refseq names}
return unless $self->load_from_embl(lc($args{-class})=>$args{-name}); $args{-class} = $self->refclass; @segments = $self->SUPER::segment(%args); } } $self->_multiple_return_args(@segments); } # default is to return 'Sequence' as the class of all references
| refclass | description | prev | next | Top |
my $self = shift; my $refname = shift; 'Accession';}
| load_from_embl | description | prev | next | Top |
my $self = shift; my $db = shift; my $acc = shift or $self->throw('Must provide an accession ID'); my $biofetch; if ($self->{_biofetch}{$db}) { $biofetch = $self->{_biofetch}{$db}; } else { $biofetch = $self->{_biofetch}{$db} = Bio::DB::BioFetch->new(-db=>$db); $biofetch->retrieval_type('tempfile'); $biofetch->proxy(@{$self->{_proxy}}) if $self->{_proxy}; } my $seq = eval {$biofetch->get_Seq_by_id($acc)} or return; $self->_load_embl($acc,$seq); 1;}
| load_from_file | description | prev | next | Top |
my $self = shift; my $file = shift; my $format = $file =~ /\.(gb|genbank|gbk)$/i ? 'genbank' : 'embl'; my $seqio = Bio::SeqIO->new( '-format' => $format, -file => $file); my $seq = $seqio->next_seq; $self->_load_embl($seq->accession,$seq); 1;}
| _load_embl | description | prev | next | Top |
my $self = shift; my $acc = shift; my $seq = shift; my $refclass = $self->refclass; my $locus = $seq->id; # begin loading}
$self->setup_load(); # first synthesize the entry for the top-level feature
my @aliases; foreach ($seq->accession,$seq->get_secondary_accessions) { next if lc($_) eq lc($acc); push @aliases,[Alias => $_]; } $self->load_gff_line( { ref => $acc, class => $refclass, source => 'EMBL', method => 'origin', start => 1, stop => $seq->length, score => undef, strand => '.', phase => '.', gclass => $self->refclass, gname => $acc, tstart => undef, tstop => undef, attributes => [[Note => $seq->desc],@aliases], } ); # now load each feature in turn
for my $feat ($seq->all_SeqFeatures) { my $attributes = $self->get_attributes($feat); my $name = $self->guess_name($attributes); my $location = $feat->location; my @segments = map {[$_->start,$_->end,$_->seq_id]} $location->can('sub_Location') ? $location->sub_Location : $location; my $type = $feat->primary_tag eq 'CDS' ? 'mRNA' : $feat->primary_tag; my $parttype = $feat->primary_tag eq 'gene' ? 'exon' : $feat->primary_tag; if ($feat->primary_tag =~ /^(gene|CDS)$/) { $self->load_gff_line( { ref => $acc, class => $refclass, source => 'EMBL', method => $type, start => $location->start, stop => $location->end, score => $feat->score || undef, strand => $feat->strand > 0 ? '+' : ($feat->strand < 0 ? '-' : '.'), phase => $feat->frame || '.', gclass => $name->[0], gname => $name->[1], tstart => undef, tstop => undef, attributes => $attributes, } ); @$attributes = (); } for my $segment (@segments) { $self->load_gff_line( { ref => $segment->[2] eq $locus ? $acc : $segment->[2], class => $refclass, source => 'EMBL', method => $parttype, start => $segment->[0], stop => $segment->[1], score => $feat->score || undef, strand => $feat->strand > 0 ? '+' : ($feat->strand < 0 ? '-' : '.'), phase => $feat->frame || '.', gclass => $name->[0], gname => $name->[1], tstart => undef, tstop => undef, attributes => $attributes, } ); } } # finish loading
$self->finish_load(); # now load the DNA
$self->load_sequence_string($acc,$seq->seq); 1;
| get_attributes | description | prev | next | Top |
my $self = shift; my $seq = shift; my @tags = $seq->all_tags or return; my @result; foreach my $tag (@tags) { foreach my $value ($seq->each_tag_value($tag)) { push @result,[$tag=>$value]; } }\@ result;}
| guess_name | description | prev | next | Top |
my $self = shift; my $attributes = shift; # remove this fix when Lincoln fixes it properly}
return ["Misc" => "Misc"] unless ($attributes); # these are arbitrary, and possibly destructive defaults
my @ordered_attributes = sort {($self->_preferred_tags->{$a->[0]} || 0) <=> ($self->_preferred_tags->{$b->[0]} || 0)} @$attributes; my $best = pop @ordered_attributes; @$attributes = @ordered_attributes; return $best; } 1;
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