Bio::Map
GeneRelative
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Summary
Bio::Map::GeneRelative - Represents being relative to named sub-regions of a
gene.
Package variables
No package variables defined.
Included modules
Scalar::Util qw ( looks_like_number )
Inherit
Synopsis
use Bio::Map::GeneRelative;
# say that a somthing will have a position relative to the start of the
# gene on map
my $rel = Bio::Map::GeneRelative->new(-gene => 0);
# or that something will be relative to the third transcript of a gene
# on a map
$rel = Bio::Map::GeneRelative->new(-transcript => 3);
# or to the 5th intron of the default transcript
$rel = Bio::Map::GeneRelative->new(-intron => [0, 5]);
# use the $rel as normal; see Bio::Map::Relative
Description
Be able to say that a given position is relative to some standard part of a
gene.
Methods
Methods description
Title : new Usage : my $relative = Bio::Map::Relative->new(); Function: Build a new Bio::Map::Relative object. Returns : Bio::Map::Relative object Args : -gene => int : coordinates are relative to the int'th base downstream of the Position's map's gene [default is gene => 0, ie. relative to the start of the gene], -transcript => int : or relative to the start of the int'th transcript of the Position's map's gene, -exon => [i, n] : or relative to the start of the n'th transcript's i'th exon, -intron => [i, n] : or intron, -coding => int : or the start of the int'th transcript's coding region.
-description => string : Free text description of what this relative
describes
(To say a Position is relative to something and upstream of it,
the Position's start() co-ordinate should be set negative)
In all cases, a transcript number of 0 means the active transcript. |
Title : absolute_conversion Usage : my $absolute_coord = $relative->absolute_conversion($pos); Function: Convert the start co-ordinate of the supplied position into a number relative to the start of its map. Returns : scalar number Args : Bio::Map::PositionI object |
Title : gene Usage : my $int = $relative->gene(); $relative->gene($int); Function: Get/set the distance from the start of the gene that the Position's co-ordiantes are relative to. Returns : int Args : none to get, OR int to set; a value of 0 means relative to the start of the gene. |
Title : transcript Usage : my $int = $relative->transcript(); $relative->transcript($int); Function: Get/set which transcript of the Position's map's gene the Position's co-ordinates are relative to. Returns : int Args : none to get, OR int to set; a value of 0 means the active (default) transcript. |
Title : exon Usage : my ($exon_number, $transcript_number) = @{$relative->exon()}; $relative->exon($exon_number, $transcript_number); Function: Get/set which exon of which transcript of the Position's map's gene the Position's co-ordinates are relative to. Returns : reference to list with two ints, exon number and transcript number Args : none to get, OR int (exon number) AND int (transcript number) to set. The second int is optional and defaults to 0 (meaning default/active transcript). |
Title : intron Usage : my ($intron_number, $transcript_number) = @{$relative->intron()}; $relative->intron($intron_number, $transcript_number); Function: Get/set which intron of which transcript of the Position's map's gene the Position's co-ordinates are relative to. Returns : reference to list with two ints, intron number and transcript number Args : none to get, OR int (intron number) AND int (transcript number) to set. The second int is optional and defaults to 0 (meaning default/active transcript). |
Title : coding Usage : my $transcript_number = $relative->coding; $relative->coding($transcript_number); Function: Get/set which transcript's coding region of the Position's map's gene the Position's co-ordinates are relative to. Returns : int Args : none to get, OR int to set (the transcript number, see transcript()) |
Methods code
sub new
{ my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($gene, $transcript, $exon, $intron, $coding) =
$self->_rearrange([qw( GENE TRANSCRIPT EXON INTRON CODING )], @args);
my $set = (defined $gene) + (defined $transcript) + (defined $exon) + (defined $intron) + (defined $coding);
if ($set > 1) {
$self->throw("-gene, -transcript, -exon, -intron and -coding are mutually exclusive");
}
if ($exon && (! ref($exon) || ref($exon) ne 'ARRAY')) {
$self->throw("-exon takes an array ref");
}
if ($intron && (! ref($intron) || ref($intron) ne 'ARRAY')) {
$self->throw("-intron takes an array ref");
}
if ($set == 0) {
if ($self->type) {
$self->warn("You set a type of relative not supported by GeneRelative; resetting to type 'gene'");
}
$gene = 0;
}
$self->gene($gene) if defined $gene;
$self->transcript($transcript) if defined $transcript;
$self->exon(@{$exon}) if defined $exon;
$self->intron(@{$intron}) if defined $intron;
$self->coding($coding) if defined $coding;
return $self;} |
sub absolute_conversion
{ my ($self, $pos) = @_;
$self->throw("Must supply an object") unless ref($pos);
$self->throw("This is [$pos], not a Bio::Map::PositionI") unless $pos->isa('Bio::Map::PositionI');
my $raw = $pos->start($pos->relative);
$self->throw("Can't convert co-ordinates when start isn't set") unless defined($raw);
my $type = $self->type;
my $value = $self->$type;
$self->throw("Details not yet set for this Relative, cannot convert") unless defined($value);
if ($type =~ /gene|transcript|exon|intron|coding/) {
my $map = $pos->map;
my $throw_desc = $type eq 'gene' ? 'gene' : "gene's transcript";
$self->throw("Relative to a map's $throw_desc, but the Position has no map") unless $map;
$self->throw("Relative to a map's $throw_desc, but the Position's map isn't a Bio::Map::GeneMap") unless $map->isa('Bio::Map::GeneMap');
my $gene = $map->gene;
if ($type eq 'gene') {
my $gene_pos = $gene->position($map);
my $rel = $gene_pos->relative;
my $start = $rel->absolute_conversion($gene_pos);
$value += $start;
}
else {
my @values = ref($value) ? @{$value} : ($value);
my $trans = ref($value) ? $values[1] : $value;
my $throw_txt = $trans == 0 ? 'default/active transcript' : "transcript $trans";
my $throw_txt2 = ref($value) ? ", or no $type $values[0]" : '';
my $method = $type eq 'coding' ? 'coding_position' : "get_${type}_position";
$value = $gene->$method($map, @values) || $self->throw("Relative to $throw_txt of the map's gene, but there is no such transcript$throw_txt2");
}
}
else {
return $self->SUPER::absolute_conversion($pos);
}
if (ref($value)) {
my $rel = $value->relative;
$value = $rel->absolute_conversion($value);
}
if (defined($value)) {
return $value + $raw;
}
return;} |
sub gene
{ my ($self, $num) = @_;
if (defined($num)) {
$self->throw("This is [$num], not a number") unless looks_like_number($num);
$self->{_use} = 'gene';
$self->{_gene} = $num;
}
return defined($self->{_gene}) ? $self->{_gene} : return;} |
sub transcript
{ my ($self, $num) = @_;
if (defined($num)) {
$self->throw("This is [$num], not a number") unless looks_like_number($num);
$self->{_use} = 'transcript';
$self->{_transcript} = $num;
}
return defined($self->{_transcript}) ? $self->{_transcript} : return;} |
sub exon
{ my ($self, $num, $t_num) = @_;
if (defined($num)) {
if (defined($t_num)) {
$self->throw("This is [$t_num], not a number") unless looks_like_number($t_num);
}
$t_num ||= 0;
$self->throw("This is [$num], not a number") unless looks_like_number($num);
$self->{_use} = 'exon';
$self->{_exon} = [$num, $t_num];
}
return $self->{_exon} || return;} |
sub intron
{ my ($self, $num, $t_num) = @_;
if (defined($num)) {
if (defined($t_num)) {
$self->throw("This is [$t_num], not a number") unless looks_like_number($t_num);
}
$t_num ||= 0;
$self->throw("This is [$num], not a number") unless looks_like_number($num);
$self->{_use} = 'intron';
$self->{_intron} = [$num, $t_num];
}
return $self->{_intron} || return;} |
sub coding
{ my ($self, $num) = @_;
if (defined($num)) {
$self->throw("This is [$num], not a number") unless looks_like_number($num);
$self->{_use} = 'coding';
$self->{_coding} = $num;
}
return defined($self->{_coding}) ? $self->{_coding} : return;
}
1;} |
General documentation
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The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Title : type
Usage : my $type = $relative->type();
Function: Get the type of thing we are relative to. The types correspond
to a method name, so the value of what we are relative to can
subsequently be found by $value = $relative->$type;
Note that type is set by the last method that was set, or during
new().
Returns : 'gene', 'transcript', 'exon', 'intron' or 'coding'
Args : none