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Summary

Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease

Package variables

Included modules

Inherit

Bio::Restriction::Enzyme

Synopsis

  # set up a single restriction enzyme. This contains lots of
  # information about the enzyme that is generally parsed from a
  # rebase file and can then be read back

  use Bio::Restriction::Enzyme;

Description

This module defines a restriction endonuclease class where one object
represents one of the distinct recognition sites for that enzyme. The
method others stores references to other objects with
alternative sites.
In this schema each object within an EnzymeCollection can be checked
for matching a sequence.
REBASE report notation Bsp24I (8/13)GACNNNNNNTGG(12/7) means:

   Bsp24I
             5'      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3'
             3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5'

Methods

Methods description

 Title     : new
 Function
 Function  : Initializes the enzyme object
 Returns   : The Restriction::Enzyme::MultiCut object
 Argument  :

 Title     : others
 Usage     : $re->others(@enz_obj_array);
 Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
 Arguments : optional array of Enzyme::MultiCut objects
 Returns   : array of Enzyme objects

 Title     : purge_others
 Usage     : $re->purge_references();
 Function  : Purges the set of references for this enzyme
 Arguments : 
 Returns   :

Methods code

sub new {

    my($class, @args) = @_;
    my $self = $class->SUPER::new(@args);

    my ($others) =
            $self->_rearrange([qw(
                                  OTHERS
                                 )], @args);

    $others && $self->others($others);
    return $self;

}

sub others {

    my $self = shift;
    push @{$self->{_others}}, @_ if @_;
    return unless $self->{_others};
    return @{$self->{'_others'}};

}

sub purge_others {

    my ($self) = shift;
    $self->{_others} = [];

}


1;

}

General documentation

User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.

Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:

  https://redmine.open-bio.org/projects/bioperl/

Heikki Lehvaslaiho, heikki-at-bioperl-dot-org

Rob Edwards, redwards@utmem.edu

Copyright (c) 2003 Rob Edwards.
Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All
Rights Reserved. This module is free software; you can redistribute
it and/or modify it under the same terms as Perl itself.

Bio::Restriction::Enzyme, Bio::Restriction::Analysis,
Bio::Restriction::EnzymeCollection

Methods beginning with a leading underscore are considered private and
are intended for internal use by this module. They are not considered
part of the public interface and are described here for documentation
purposes only.