Bio::Search::Hit
ModelHit
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Summary
Bio::Search::Hit::ModelHit - A model-based implementation of the Bio::Search::Hit::HitI interface
Package variables
No package variables defined.
Inherit
Synopsis
use Bio::Search::Hit::ModelHit;
my $hit = Bio::Search::Hit::ModelHit->new(-algorithm => 'rnamotif');
# typically one gets HitI objects from a SearchIO stream via a ResultI
use Bio::SearchIO;
my $parser = Bio::SearchIO->new(-format => 'infernal', -file => 'trap.inf');
my $result = $parser->next_result;
my $hit = $result->next_hit;
Description
This object handles the hit data from a database search using models or
descriptors instead of sequences, such as Infernal, HMMER, RNAMotif, etc.
Unless you're writing a parser, you won't ever need to create a ModelHit or
any other HitI-implementing object. If you use the SearchIO system, HitI objects
are created automatically from a SearchIO stream which returns
Bio::Search::Hit::HitI objects.
Note that several HitI-based methods have been overridden from ModelHit due to
their unreliability when dealing with queries that aren't sequence-based. It may
be possible to reimplement these at a later point, but for the time being they
will throw warnings and return w/o results.
For documentation on what you can do with ModelHit (and other HitI objects),
please see the API documentation in
Bio::Search::Hit::HitI.
Methods
Methods description
Usage : $hit_object->n(); Purpose : Gets the N number for the current hit. : This is the number of HSPs in the set which was ascribed : the lowest P-value (listed on the description line). : This number is not the same as the total number of HSPs. : To get the total number of HSPs, use num_hsps(). Example : $n = $hit_object->n(); Returns : Integer Argument : n/a Throws : Exception if HSPs have not been set. Comments : Calling n() on such reports will result in a call to num_hsps(). : The num_hsps() method will count the actual number of : HSPs in the alignment listing, which may exceed N in : some cases.
See Also : num_hsps() |
Usage : $hit_object->hsp( [string] ); Purpose : Get a single HSPI object for the present HitI object. Example : $hspObj = $hit_object->hsp; # same as 'best' : $hspObj = $hit_object->hsp('best'); : $hspObj = $hit_object->hsp('worst'); Returns : Object reference for a Bio::Search::HSP::BlastHSP.pm object. Argument : String (or no argument). : No argument (default) = highest scoring HSP (same as 'best'). : 'best' or 'first' = highest scoring HSP. : 'worst' or 'last' = lowest scoring HSP. Throws : Exception if the HSPs have not been collected. : Exception if an unrecognized argument is used.
See Also : hsps(), num_hsps() |
Title : rank Usage : $obj->rank($newval) Function: Get/Set the rank of this Hit in the Query search list i.e. this is the Nth hit for a specific query Returns : value of rank Args : newvalue (optional) |
Title : locus Usage : $locus = $hit->locus(); Function: Retrieve the locus (if available) for the hit Returns : a scalar string (empty string if not set) Args : none |
Title : each_accession_number Usage : @each_accession_number = $hit->each_accession_number(); Function: Get each accession number listed in the description of the hit. If there are no alternatives, then only the primary accession will be given Returns : list of all accession numbers in the description Args : none |
Title : query_length Usage : $obj->query_length($newval) Function: Get/Set the query_length Returns : value of query_length (a scalar) Args : on set, new value (a scalar or undef, optional) |
Title : ncbi_gi Usage : $acc = $hit->ncbi_gi(); Function: Retrieve the NCBI Unique ID (aka the GI #), if available, for the hit Returns : a scalar string (empty string if not set) Args : none |
Methods code
sub overlap
{ my $self = shift;
if(@_) { $self->{'_overlap'} = shift; }
defined $self->{'_overlap'} ? $self->{'_overlap'} : 0;} |
sub n
{ my $self = shift;
if (defined @_) {
$self->{'_n'} = shift;
}
if (defined $self->{'_n'}) {
return $self->{'_n'}
} else {
return $self->num_hsps;
}} |
sub hsp
{ my( $self, $option ) = @_;
$option ||= 'best';
if (not ref $self->{'_hsps'}) {
$self->throw("Can't get HSPs: data not collected.");
}
my @hsps = $self->hsps;
return $hsps[0] if $option =~ /best|first|1/i;
return $hsps[$#hsps] if $option =~ /worst|last/i;
$self->throw("Can't get HSP for: $option\n" .
"Valid arguments: 'best', 'worst'");} |
sub rank
{ my $self = shift;
return $self->{'_rank'} = shift if @_;
return $self->{'_rank'} || 1;} |
sub locus
{ my ($self,$value) = @_;
my $previous = $self->{'_locus'};
if( defined $value || ! defined $previous ) {
unless (defined $value) {
if ($self->{'_name'} =~/(gb|emb|dbj|ref)\|(.*)\|(.*)/) {
$value = $previous = $3;
} else {
$value = $previous = '';
}
}
$self->{'_locus'} = $value;
}
return $previous;} |
sub each_accession_number
{ my ($self,$value) = @_;
my $desc = $self->{'_description'};
my @accnums;
push (@accnums,$self->{'_accession'});
if( defined $desc ) {
while ($desc =~ /(\b\S+\|\S*\|\S*\s?)/g) {
my $id = $1;
my ($acc, $version);
if ($id =~ /(gb|emb|dbj|sp|pdb|bbs|ref|tp[gde])\|(.*)\|(.*)/) {
($acc, $version) = split /\./, $2;
} elsif ($id =~ /(pir|prf|pat|gnl)\|(.*)\|(.*)/) {
($acc, $version) = split /\./, $3;
} elsif( $id =~ /(gim|gi|bbm|bbs|lcl)\|(\d*)/) {
$acc = $id;
} elsif( $id =~ /(oth)\|(.*)\|(.*)\|(.*)/ ) { ($acc,$version) = ($2);
} else {
$acc=$id;
}
push(@accnums, $acc);
}
}
return @accnums;} |
sub query_length
{ my $self = shift;
return $self->{'_query_length'} = shift if @_;
return $self->{'_query_length'};} |
sub ncbi_gi
{ my ($self,$value) = @_;
my $previous = $self->{'_ncbi_gi'};
if( defined $value || ! defined $previous ) {
$value = $previous = '' unless defined $value;
$self->{'_ncbi_gi'} = $value;
}
return $previous;} |
sub length_aln
{ my $self = shift;
$self->warn('$hit->length_aln not implemented for Model-based searches');
return;} |
sub gaps
{ my $self = shift;
$self->warn('$hit->gaps not implemented for Model-based searches');
return;} |
sub matches
{ my $self = shift;
$self->warn('$hit->matches not implemented for Model-based searches');
return;} |
sub start
{ my $self = shift;
$self->warn('$hit->start not implemented for Model-based searches');
return;} |
sub end
{ my $self = shift;
$self->warn('$hit->end not implemented for Model-based searches');
return;} |
sub range
{ my $self = shift;
$self->warn('$hit->range not implemented for Model-based searches');
return;} |
sub frac_identical
{ my $self = shift;
$self->warn('$hit->frac_identical not implemented for Model-based searches');
return;} |
sub frac_conserved
{ my $self = shift;
$self->warn('$hit->frac_conserved not implemented for Model-based searches');
return;} |
sub frac_aligned_query
{ my $self = shift;
$self->warn('$hit->frac_aligned_query not implemented for Model-based searches');
return;} |
sub frac_aligned_hit
{ my $self = shift;
$self->warn('$hit->frac_aligned_hit not implemented for Model-based searches');
return;} |
sub num_unaligned_hit
{ my $self = shift;
$self->warn('$hit->num_unaligned_hit/num_unaligned_sbjct not implemented for Model-based searches');
return;} |
sub num_unaligned_query
{ my $self = shift;
$self->warn('$hit->num_unaligned_query not implemented for Model-based searches');
return;} |
sub seq_inds
{ my $self = shift;
$self->warn('$hit->seq_inds not implemented for Model-based searches');
return;} |
sub strand
{ my $self = shift;
$self->warn('$hit->strand not implemented for Model-based searches');
return;} |
sub frame
{ my $self = shift;
$self->warn('$hit->frame not implemented for Model-based searches');
return;} |
sub logical_length
{ my $self = shift;
$self->warn('$hit->logical_length not implemented for Model-based searches');
return;
}
1;} |
General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
https://redmine.open-bio.org/projects/bioperl/
Email cjfields at bioperl dot org
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
| HitI methods implemented in parent class Bio::Search::Hit::ModelHit | Top |
Title : new
Usage : my $obj = Bio::Search::Hit::ModelHit->new();
Function: Builds a new Bio::Search::Hit::ModelHit object
Returns : Bio::Search::Hit::ModelHit
Args : -name => Name of Hit (required)
-description => Description (optional)
-accession => Accession number (optional)
-ncbi_gi => NCBI GI UID (optional)
-length => Length of the Hit (optional)
-score => Raw Score for the Hit (optional)
-bits => Bit Score for the Hit (optional)
-significance => Significance value for the Hit (optional)
-algorithm => Algorithm used (BLASTP, FASTX, etc...)
-hsps => Array ref of HSPs for this Hit.
-found_again => boolean, true if hit appears in a
"previously found" section of a PSI-Blast report.
-hsp_factory => Bio::Factory::ObjectFactoryI able to create HSPI
objects.
Title : add_hsp
Usage : $hit->add_hsp($hsp)
Function: Add a HSP to the collection of HSPs for a Hit
Returns : number of HSPs in the Hit
Args : Bio::Search::HSP::HSPI object, OR hash ref containing data suitable
for creating a HSPI object (&hsp_factory must be set to get it back)
Title : hsp_factory
Usage : $hit->hsp_factory($hsp_factory)
Function: Get/set the factory used to build HSPI objects if necessary.
Returns : Bio::Factory::ObjectFactoryI
Args : Bio::Factory::ObjectFactoryI
| Bio::Search::Hit::HitI methods | Top |
Implementation of Bio::Search::Hit::HitI methods
Title : name
Usage : $hit_name = $hit->name();
Function: returns the name of the Hit sequence
Returns : a scalar string
Args : [optional] scalar string to set the name
Title : accession
Usage : $acc = $hit->accession();
Function: Retrieve the accession (if available) for the hit
Returns : a scalar string (empty string if not set)
Args : none
Title : description
Usage : $desc = $hit->description();
Function: Retrieve the description for the hit
Returns : a scalar string
Args : [optional] scalar string to set the descrition
Title : length
Usage : my $len = $hit->length
Function: Returns the length of the hit
Returns : integer
Args : [optional] integer to set the length
Title : algorithm
Usage : $alg = $hit->algorithm();
Function: Gets the algorithm specification that was used to obtain the hit
For BLAST, the algorithm denotes what type of sequence was aligned
against what (BLASTN: dna-dna, BLASTP prt-prt, BLASTX translated
dna-prt, TBLASTN prt-translated dna, TBLASTX translated
dna-translated dna).
Returns : a scalar string
Args : [optional] scalar string to set the algorithm
Title : raw_score
Usage : $score = $hit->raw_score();
Function: Gets the "raw score" generated by the algorithm. What
this score is exactly will vary from algorithm to algorithm,
returning undef if unavailable.
Returns : a scalar value
Args : [optional] scalar value to set the raw score
Title : significance
Usage : $significance = $hit->significance();
Function: Used to obtain the E or P value of a hit, i.e. the probability that
this particular hit was obtained purely by random chance. If
information is not available (nor calculatable from other
information sources), return undef.
Returns : a scalar value or undef if unavailable
Args : [optional] scalar value to set the significance
Usage : $hit_object->bits();
Purpose : Gets the bit score of the best HSP for the current hit.
Example : $bits = $hit_object->bits();
Returns : Integer or undef if bit score is not set
Argument : n/a
Comments : For BLAST1, the non-bit score is listed in the summary line.
See Also :
score()
Title : next_hsp
Usage : while( $hsp = $obj->next_hsp()) { ... }
Function : Returns the next available High Scoring Pair
Example :
Returns : Bio::Search::HSP::HSPI object or null if finished
Args : none
Usage : $hit_object->hsps();
Purpose : Get a list containing all HSP objects.
: Get the numbers of HSPs for the current hit.
Example : @hsps = $hit_object->hsps();
: $num = $hit_object->hsps(); # alternatively, use num_hsps()
Returns : Array context : list of Bio::Search::HSP::BlastHSP.pm objects.
: Scalar context: integer (number of HSPs).
: (Equivalent to num_hsps()).
Argument : n/a. Relies on wantarray
Throws : Exception if the HSPs have not been collected.
See Also :
hsp(),
num_hsps()
Usage : $hit_object->num_hsps();
Purpose : Get the number of HSPs for the present hit.
Example : $nhsps = $hit_object->num_hsps();
Returns : Integer or '-' if HSPs have not been callected
Argument : n/a
See Also :
hsps()
Title : rewind
Usage : $hit->rewind;
Function: Allow one to reset the HSP iterator to the beginning
Since this is an in-memory implementation
Returns : none
Args : none
Usage : $ambig_code = $hit_object->ambiguous_aln();
Purpose : Sets/Gets ambiguity code data member.
Example : (see usage)
Returns : String = 'q', 's', 'qs', '-'
: 'q' = query sequence contains overlapping sub-sequences
: while sbjct does not.
: 's' = sbjct sequence contains overlapping sub-sequences
: while query does not.
: 'qs' = query and sbjct sequence contains overlapping sub-sequences
: relative to each other.
: '-' = query and sbjct sequence do not contains multiple domains
: relative to each other OR both contain the same distribution
: of similar domains.
Argument : n/a
Throws : n/a
Comment : Note: "sbjct" is synonymous with "hit"
Usage : $hit_object->p( [format] );
Purpose : Get the P-value for the best HSP
Example : $p = $sbjct->p;
: $p = $sbjct->p('exp'); # get exponent only.
: ($num, $exp) = $sbjct->p('parts'); # split sci notation into parts
Returns : Float or scientific notation number (the raw P-value, DEFAULT).
: Integer if format == 'exp' (the magnitude of the base 10 exponent).
: 2-element list (float, int) if format == 'parts' and P-value
: is in scientific notation (See Comments).
Argument : format: string of 'raw' | 'exp' | 'parts'
: 'raw' returns value given in report. Default. (1.2e-34)
: 'exp' returns exponent value only (34)
: 'parts' returns the decimal and exponent as a
: 2-element list (1.2, -34) (See Comments).
Throws : Warns if no P-value is defined. Uses expect instead.
Comments : Using the 'parts' argument is not recommended since it will not
: work as expected if the P-value is not in scientific notation.
: That is, floats are not converted into sci notation before
: splitting into parts.
See Also :
expect(),
signif(),
Bio::Search::SearchUtils::get_exponent()
| ModelHit methods overridden in ModelHit | Top |
The following methods have been overridden due to their current reliance on
sequence-based queries. They may be implemented in future versions of this class.