Bio::SearchIO hmmer3
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Summary
Bio::SearchIO::hmmer3 - DESCRIPTION of Object
Package variables
Globals (from "use vars" definitions)
%MAPPING
%MODEMAP
Included modules
Bio::Factory::ObjectFactory
Data::Dumper
Inherit
Bio::SearchIO::hmmer
Synopsis
Give standard usage here
Description
Describe the object here
Methods
BEGIN Code
next_resultDescriptionCode
start_elementDescriptionCode
end_elementDescriptionCode
elementDescriptionCode
charactersDescriptionCode
within_elementDescriptionCode
in_elementDescriptionCode
start_documentDescriptionCode
end_documentDescriptionCode
result_countDescriptionCode
Methods description
next_resultcode    nextTop
 Title   : next_result
Usage : my $hit = $searchio->next_result;
Function: Returns the next Result from a search
Returns : Bio::Search::Result::ResultI object
Args : none
start_elementcodeprevnextTop
 Title   : start_element
Usage : $eventgenerator->start_element
Function: Handles a start event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name'
end_elementcodeprevnextTop
 Title   : end_element
Usage : $eventgeneartor->end_element
Function: Handles and end element event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name'
elementcodeprevnextTop
 Title   : element
Usage : $eventhandler->element({'Name' => $name, 'Data' => $str});
Function: Convienence method that calls start_element, characters, end_element
Returns : none
Args : Hash ref with the keys 'Name' and 'Data'
characterscodeprevnextTop
 Title   : characters
Usage : $eventgenerator->characters($str)
Function: Send a character events
Returns : none
Args : string
within_elementcodeprevnextTop
 Title   : within_element
Usage : if( $eventgenerator->within_element( $element ) ) {}
Function: Test if we are within a particular element
This is different than 'in' because within can be tested for
a whole block
Returns : boolean
Args : string element name
in_elementcodeprevnextTop
 Title   : in_element
Usage : if( $eventgenerator->in_element( $element ) ) {}
Function: Test if we are in a particular element
This is different than 'within' because 'in' only
tests its immediate parent
Returns : boolean
Args : string element name
start_documentcodeprevnextTop
 Title   : start_document
Usage : $eventgenerator->start_document
Function: Handle a start document event
Returns : none
Args : none
end_documentcodeprevnextTop
 Title   : end_document
Usage : $eventgenerator->end_document
Function: Handles an end document event
Returns : Bio::Search::Result::ResultI object
Args : none
result_countcodeprevnextTop
 Title   : result_count
Usage : my $count = $searchio->result_count
Function: Returns the number of results processed
Returns : interger
Args : none
Methods code
BEGINTop
BEGIN {
    # mapping of HMMER items to Bioperl hash keys
%MODEMAP = ( 'HMMER_Output' => 'result', 'Hit' => 'hit', 'Hsp' => 'hsp' ); %MAPPING = ( 'Hsp_bit-score' => 'HSP-bits', 'Hsp_score' => 'HSP-score', 'Hsp_evalue' => 'HSP-evalue', 'Hsp_query-from' => 'HSP-query_start', 'Hsp_query-to' => 'HSP-query_end', 'Hsp_hit-from' => 'HSP-hit_start', 'Hsp_hit-to' => 'HSP-hit_end', 'Hsp_positive' => 'HSP-conserved', 'Hsp_identity' => 'HSP-identical', 'Hsp_gaps' => 'HSP-hsp_gaps', 'Hsp_hitgaps' => 'HSP-hit_gaps', 'Hsp_querygaps' => 'HSP-query_gaps', 'Hsp_qseq' => 'HSP-query_seq', 'Hsp_hseq' => 'HSP-hit_seq', 'Hsp_midline' => 'HSP-homology_seq', 'Hsp_align-len' => 'HSP-hsp_length', 'Hsp_query-frame' => 'HSP-query_frame', 'Hsp_hit-frame' => 'HSP-hit_frame', 'Hit_id' => 'HIT-name', 'Hit_len' => 'HIT-length', 'Hit_accession' => 'HIT-accession', 'Hit_desc' => 'HIT-description', 'Hit_signif' => 'HIT-significance', 'Hit_score' => 'HIT-score', 'HMMER_program' => 'RESULT-algorithm_name', 'HMMER_version' => 'RESULT-algorithm_version', 'HMMER_query-def' => 'RESULT-query_name', 'HMMER_query-len' => 'RESULT-query_length', 'HMMER_query-acc' => 'RESULT-query_accession', 'HMMER_querydesc' => 'RESULT-query_description', 'HMMER_hmm' => 'RESULT-hmm_name', 'HMMER_seqfile' => 'RESULT-sequence_file', 'HMMER_db' => 'RESULT-database_name', );
}
next_resultdescriptionprevnextTop
sub next_result {
   my ($self)  = @_;
   my $seentop = 0; #Placeholder for when we deal with multi-query reports
my $reporttype; my ( $last, @hit_list, @hsp_list, %hspinfo, %hitinfo, %domaincounter ); local $/ = "\n"; local $_; my $verbose = $self->verbose; # cache for speed? JES's idea in hmmer.pm
$self->start_document(); local ($_); #This is here to ensure that next_result doesn't produce infinite loop
if(!defined( $_ = $self->_readline) ) { return undef; } else{ $self->_pushback($_); } #Regex goes here for HMMER3
#Start with hmmsearch processing
while ( defined( $_ = $self->_readline ) ) { my $lineorig = $_; chomp; #Grab the program name.
if ( $_ =~ m/^\#\s(\S+)\s\:\:\s/ ){
my
$prog = $1;
#TO DO LATER: customize the above regex to adapt to other
#program types!!! (hmmscan, etc)
$self->start_element( { 'Name' => 'HMMER_Output' } ); $self->{'_result_count'}++; #Might need to move to another block
$self->element( { 'Name' => 'HMMER_program', 'Data' => uc($prog) } ); } #Get the HMMER package version and release date
elsif ( $_ =~ m/^\#\sHMMER\s+(\S+)\s+\((.+)\)/ ) {
my
$version = $1;
my $versiondate = $2; $self->{'_hmmidline'} = $_; $self->element( { 'Name' => 'HMMER_version', 'Data' => $version } ); } #Get the query info
elsif( $_ =~ /^\#\squery \w+ file\:\s+(\S+)/ ){ if( $self->{'_reporttype'} eq 'HMMSEARCH') { $self->{'_hmmfileline'} = $lineorig; $self->element( { 'Name' => 'HMMER_hmm', 'Data' => $1 } ); } elsif( $self->{'_reporttype'} eq 'HMMSCAN' ) { $self->{'_hmmseqline'} = $lineorig; $self->element( { 'Name' => 'HMMER_seqfile', 'Data' => $1 } ); } } #If this is a report without alignments
elsif( $_ =~ m/^\#\sshow\salignments\sin\soutput/ ){
$self->{'_alnreport'} = 0;
} #Get the database info
elsif( $_ =~ m/^\#\starget\s\S+\sdatabase\:\s+(\S+)/ ){
#
$self->{'_hmmseqline'} = $lineorig;
# $self->element(
# {
# 'Name' => 'HMMER_seqfile',
# 'Data' => $1
# }
# );
if( $self->{'_reporttype'} eq 'HMMSEARCH') { $self->{'_hmmseqline'} = $lineorig; $self->element( { 'Name' => 'HMMER_seqfile', 'Data' => $1 } ); } elsif( $self->{'_reporttype'} eq 'HMMSCAN' ) { $self->{'_hmmfileline'} = $lineorig; $self->element( { 'Name' => 'HMMER_hmm', 'Data' => $1 } ); } } #Get query data
elsif( $_ =~ s/^Query:\s+// ) { #TODO Code to deal with multi query report
unless( s/\s+\[[L|M]\=(\d+)\]$// ){ warn "Error parsing length for query, offending line $_\n"; exit(0); } my $querylen = $1; $self->element( { 'Name' => 'HMMER_query-len', 'Data' => $querylen } ); $self->element( { 'Name' => 'HMMER_query-def', 'Data' => $_ } ); } #Get Accession data
elsif( $_ =~ s/^Accession:\s+// ){ s/\s+$//; $self->element( { 'Name' => 'HMMER_query-acc', 'Data' => $_ } ); } #Get description data
elsif( $_ =~ s/^Description:\s+// ){ s/\s+$//; $self->element( { 'Name' => 'HMMER_querydesc', 'Data' => $_ } ); } #PROCESS HMMSEARCH AND HMMSCAN RESULTS SPECIFIC FORMATTING HERE
elsif( defined $self->{'_reporttype'} && ( $self->{'_reporttype'} eq 'HMMSEARCH' || $self->{'_reporttype'} eq 'HMMSCAN' ) ){ #Complete sequence table data above inclusion threshold
if( $_ =~ m/Scores for complete sequence/){
while (defined(
$_ = $self->_readline ) ) {
if (
$_ =~ m/inclusion threshold/ || m/Domain( and alignment)? annotation for each/ ||
m/\[No hits detected/ || m!^//! ){
$self->_pushback($_);
last; } #grab table data
next if ( m/\-\-\-/ || m/^\s+E\-value\s+score/ || m/^$/);
my ( $eval_full, $score_full, $bias_full, $eval_best, $score_best, $bias_best, $exp, $n, $hitid, $desc, @hitline ); @hitline = split(" ", $_); $eval_full = shift @hitline; $score_full = shift @hitline; $bias_full = shift @hitline; $eval_best = shift @hitline; $score_best = shift @hitline; $bias_best = shift @hitline; $exp = shift @hitline; $n = shift @hitline; $hitid = shift @hitline; $desc = join " ", @hitline; if( !defined( $desc ) ){ $desc = ""; } push @hit_list, [ $hitid, $desc, $eval_full, $score_full ]; $hitinfo{$hitid}= $#hit_list; } } #Complete sequence table data below inclusion threshold
#not currently fully implemented
elsif( $_ =~ m/inclusion threshold/ ){
while( defined(
$_ = $self->_readline ) ) {
if(
$_ =~ m/Domain( and alignment)? annotation for each/ ||
m/Internal pipeline statistics summary/ ){
$self->_pushback($_);
last; } next if( $_ =~ m/^$/ );
my ( $eval_full, $score_full, $bias_full, $eval_best, $score_best, $bias_best, $exp, $n, $hitid, $desc, @hitline ); @hitline = split(" ", $_); $eval_full = shift @hitline; $score_full = shift @hitline; $bias_full = shift @hitline; $eval_best = shift @hitline; $score_best = shift @hitline; $bias_best = shift @hitline; $exp = shift @hitline; $n = shift @hitline; $hitid = shift @hitline; $desc = join " ", @hitline; $hitinfo{$hitid} = "below_inclusion"; } } #Domain annotation for each sequence table data
elsif( $_ =~ m/Domain( and alignment)? annotation for each/){
@hsp_list = (); #here for multi-query reports
my
$name;
while( defined( $_ = $self->_readline ) ) { if ($_ =~ m/Internal pipeline statistics/ || m/\[No targets detected/ ){
$self->_pushback($_);
last; } if( $_ =~ m/^\>\>\s(.*?)\s+/ ) {
$name = $1;
#skip hits below inclusion threshold
next if( $hitinfo{$name} eq "below_inclusion"); $domaincounter{$name} = 0; while( defined( $_ = $self->_readline ) ) { #grab table data for sequence
if ($_ =~ m/Internal pipeline statistics/ ||
$_ =~ m/^\>\>/ ){
$self->_pushback($_);
last; } if ( $_ =~ m/Alignments for each domain/ ) {
$self->_pushback($_);
last; } if ( $_ =~ m/^\s+\#\s+score/ ||
$_ =~ m/^\s\-\-\-\s+/ ||
#
$_ =~ m/^\>\>/ ||
$_ =~ m/^$/ ){
next;
} # grab hsp data from table, push into @hsp;
if( my ($domain_num, $score, $bias, $ceval, $ieval, $hmmstart, $hmmstop, $qalistart, $qalistop, $envstart, $envstop, $envbound, $acc) = m!^\s+(\d+)\s\!*\?*\s+ #domain num
(\S+)\s+(\S+)\s+ #score, bias
(\S+)\s+(\S+)\s+ #c-eval, i-eval
(\d+)\s+(\d+).+? #hmm start, stop
(\d+)\s+(\d+).+? #query start, stop
(\d+)\s+(\d+).+? #env start, stop
(\S+) #acc
\s*$!ox
){
#keeping simple for now. let's customize later
my @vals = ($hmmstart, $hmmstop, $qalistart, $qalistop, $score, $ceval, '', '', '');
my $info = $hit_list[ $hitinfo{$name} ]; if( !defined $info ){ $self->warn( "Incomplete sequence information; can't find $name, hitinfo says $hitinfo{$name}\n" ); next; } $domaincounter{$name}++; my $hsp_key = $name . "_" . $domaincounter{$name}; push @hsp_list, [ $name, @vals ]; $hspinfo{$hsp_key} = $#hsp_list; } else{ print "missed this line: $_\n"; } } } elsif ($_ =~ m/Alignments for each domain/ ) {
my
$domain_count = 0;
#line counter
my $count = 0; # There's an optional block, so we sometimes need to
# count to 3, and sometimes to 4.
my $max_count = 3; my $lastdomain; my $hsp; my ($hline, $midline, $qline); while( defined( $_ = $self->_readline ) ) { if( $_ =~ m/^\>\>/ ||
$_ =~ m/Internal pipeline statistics/){
$self->_pushback($_);
last; } elsif( $hitinfo{$name} eq "below_inclusion" || $_ =~ m/^$/ ) {
next;
} elsif( $_ =~ /\s\s\=\=\sdomain\s(\d+)\s+/){ my $domainnum = $1; $count = 0; my $key = $name . "_" . $domainnum; $hsp = $hsp_list[ $hspinfo{$key} ]; $hline = $$hsp[-3]; $midline = $$hsp[-2]; $qline = $$hsp[-1]; $lastdomain = $name; } # model data track, some reports don't have
elsif( $_ =~ m/\s+\S+\sCS$/ ){
my $modeltrack = $_;
$max_count++; $count++; next; } elsif( $count == $max_count - 3 ){ #hit sequence
my @data = split(" ", $_); my $seq = $data[-2]; $hline .= $seq; $count++; next; } elsif( $count == $max_count - 2 ){ #conservation track
#storage isn't quite right - need to remove
#leading/lagging whitespace while preserving
#gap data (latter isn't done, former is)
$_ =~ s/^\s+//; $_ =~ s/\s+$//; $midline .= $_; $count++; next; } elsif( $count == $max_count - 1 ){ #query track
my @data = split(" ", $_); my $seq = $data[-2]; $qline .= $seq; $count++; next; } elsif( $count == $max_count ){ #pval track
my $pvals = $_; $count = 0; $max_count = 3; $$hsp[-3] = $hline; $$hsp[-2] = $midline; $$hsp[-1] = $qline; next; } else{ print "missed $_\n"; } } } } } elsif( m/Internal pipeline statistics/ || m!^//! ){
# if within hit, hsp close;
if ( $self->within_element('hit') ) { if ( $self->within_element('hsp') ) { $self->end_element( { 'Name' => 'Hsp' } ); } $self->end_element( { 'Name' => 'Hit' } ); } #grab summary statistics of run
while( defined( $_ = $self->_readline ) ) { last if ( $_ =~ m/^\/\/$/ );
} #Jason does a lot of processing of hits/hsps here;
while( my $hit = shift @hit_list ) { my $hit_name = shift @$hit; my $hit_desc = shift @$hit; my $hit_signif = shift @$hit; my $hit_score = shift @$hit; my $num_domains = $domaincounter{$hit_name} || 0; $self->start_element( { 'Name' => 'Hit' } ); $self->element( { 'Name' => 'Hit_id', 'Data' => $hit_name } ); $self->element( { 'Name' => 'Hit_desc', 'Data' => $hit_desc } ); $self->element( { 'Name' => 'Hit_signif', 'Data' => $hit_signif } ); $self->element( { 'Name' => 'Hit_score', 'Data' => $hit_score } ); for my $i (1..$num_domains) { my $key = $hit_name . "_" . $i; my $hsp = $hsp_list[ $hspinfo{$key} ]; if(defined $hsp) { my $hsp_name = shift @$hsp; $self->start_element( { 'Name' => 'Hsp' } ); $self->element( { 'Name' => 'Hsp_identity', 'Data' => 0 } ); $self->element( { 'Name' => 'Hsp_positive', 'Data' => 0 } ); $self->element( { 'Name' => 'Hsp_hit-from', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_hit-to', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_query-from', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_query-to', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_score', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_evalue', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_hseq', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_midline', 'Data' => shift @$hsp } ); $self->element( { 'Name' => 'Hsp_qseq', 'Data' => shift @$hsp } ); $self->end_element( { 'Name' => 'Hsp' } ); } } $self->end_element( { 'Name' => 'Hit' } ); } @hit_list = (); %hitinfo = (); last; } } else{ print "missed: $_\n"; $self->debug($_); } $last = $_; } $self->end_element( { 'Name' => 'HMMER_Output' } ); my $result = $self->end_document(); return $result;
}
start_elementdescriptionprevnextTop
sub start_element {
    my ( $self, $data ) = @_;

    # we currently don't care about attributes
my $nm = $data->{'Name'}; my $type = $MODEMAP{$nm}; if ($type) { if ( $self->_eventHandler->will_handle($type) ) { my $func = sprintf( "start_%s", lc $type ); $self->_eventHandler->$func( $data->{'Attributes'} ); } unshift @{ $self->{'_elements'} }, $type; } if ( defined $type && $type eq 'result' ) { $self->{'_values'} = {}; $self->{'_result'} = undef; }
}
end_elementdescriptionprevnextTop
sub end_element {
    my ( $self, $data ) = @_;
    my $nm   = $data->{'Name'};
    my $type = $MODEMAP{$nm};
    my $rc;

    if ( $nm eq 'HMMER_program' ) {
        if ( $self->{'_last_data'} =~ /(HMM\S+)/i ) {
            $self->{'_reporttype'} = uc $1;
        }
    }

    # Hsp are sort of weird, in that they end when another
# object begins so have to detect this in end_element for now
if ( $nm eq 'Hsp' ) { foreach (qw(Hsp_qseq Hsp_midline Hsp_hseq)) { my $data = $self->{'_last_hspdata'}->{$_}; if ($data && $_ eq 'Hsp_hseq') { # replace hmm '.' gap symbol by '-'
$data =~ s/\./-/g; } $self->element( { 'Name' => $_, 'Data' => $data } ); } $self->{'_last_hspdata'} = {}; } if ($type) { if ( $self->_eventHandler->will_handle($type) ) { my $func = sprintf( "end_%s", lc $type ); $rc = $self->_eventHandler->$func( $self->{'_reporttype'}, $self->{'_values'} ); } my $lastelem = shift @{ $self->{'_elements'} }; } elsif ( $MAPPING{$nm} ) { if ( ref( $MAPPING{$nm} ) =~ /hash/i ) { my $key = ( keys %{ $MAPPING{$nm} } )[0]; $self->{'_values'}->{$key}->{ $MAPPING{$nm}->{$key} } = $self->{'_last_data'}; } else { $self->{'_values'}->{ $MAPPING{$nm} } = $self->{'_last_data'}; # print "lastdata is " . $self->{'_last_data'} . "\n";
} } else { $self->debug("unknown nm $nm, ignoring\n"); } $self->{'_last_data'} = ''; # remove read data if we are at
# end of an element
$self->{'_result'} = $rc if ( defined $type && $type eq 'result' ); return $rc;
}
elementdescriptionprevnextTop
sub element {
    my ( $self, $data ) = @_;
    $self->start_element($data);
    $self->characters($data);
    $self->end_element($data);
}
charactersdescriptionprevnextTop
sub characters {
    my ( $self, $data ) = @_;

    if (   $self->in_element('hsp')
        && $data->{'Name'} =~ /Hsp\_(qseq|hseq|midline)/o
        && defined $data->{'Data'} )
    {
        $self->{'_last_hspdata'}->{ $data->{'Name'} } .= $data->{'Data'};
    }
    return unless ( defined $data->{'Data'} && $data->{'Data'} !~ /^\s+$/o );

    $self->{'_last_data'} = $data->{'Data'};
}
within_elementdescriptionprevnextTop
sub within_element {
    my ( $self, $name ) = @_;
    return 0
      if ( !defined $name
        || !defined $self->{'_elements'}
        || scalar @{ $self->{'_elements'} } == 0 );
    foreach ( @{ $self->{'_elements'} } ) {
        return 1 if ( $_ eq $name );
    }
    return 0;
}
in_elementdescriptionprevnextTop
sub in_element {
    my ( $self, $name ) = @_;
    return 0 if !defined $self->{'_elements'}->[0];
    return ( $self->{'_elements'}->[0] eq $name );
}
start_documentdescriptionprevnextTop
sub start_document {
   my ($self) = @_;
   $self->{'_lasttype'} = '';
   $self->{'_values'}   = {};
   $self->{'_result'}   = undef;
   $self->{'_elements'} = [];
}
end_documentdescriptionprevnextTop
sub end_document {
   my ($self) = @_;
   return $self->{'_result'};
}
result_countdescriptionprevnextTop
sub result_count {
   my $self = shift;
   return $self->{'_result_count'};
}


1;
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
  bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
SupportTop
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
  https://redmine.open-bio.org/projects/bioperl/
AUTHOR - Thomas SharptonTop
Email thomas.sharpton@gmail.com
Describe contact details here
CONTRIBUTORSTop
Additional contributors names and emails here
APPENDIXTop
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _