Bio::Tools::Run::Alignment Kalign
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Summary
Bio::Tools::Run::Alignment::Kalign - Object for the calculation of an
iterative multiple sequence alignment from a set of unaligned
sequences or alignments using the KALIGN program
Package variables
No package variables defined.
Included modules
Bio::AlignIO
Bio::Factory::ApplicationFactoryI
Bio::Root::IO
Bio::Root::Root
Bio::Seq
Bio::SeqIO
Bio::SimpleAlign
Bio::Tools::Run::WrapperBase
Inherit
Bio::Factory::ApplicationFactoryI Bio::Root::Root Bio::Tools::Run::WrapperBase
Synopsis
  # Build a kalign alignment factory
  $factory = Bio::Tools::Run::Alignment::Kalign->new(@params);

  # Pass the factory a list of sequences to be aligned.
  $inputfilename = 't/cysprot.fa';
  # $aln is a SimpleAlign object.
  $aln = $factory->align($inputfilename);

  # or where @seq_array is an array of Bio::Seq objects
  $seq_array_ref = \@seq_array;
  $aln = $factory->align($seq_array_ref);

  # Or one can pass the factory a pair of (sub)alignments
  #to be aligned against each other, e.g.:

  #There are various additional options and input formats available.
  #See the DESCRIPTION section that follows for additional details.
Description
Please cite:
        Timo Lassmann and Erik L.L. Sonnhammer (2005)
        Kalign - an accurate and fast multiple sequence alignment algorithm.
        BMC Bioinformatics 6:298
http://msa.cgb.ki.se/downloads/kalign/current.tar.gz You will need to enable Kalign to find the kalign program. This can be
done in (at least) three ways:
  1. Make sure the kalign executable is in your path (i.e. 
     'which kalign' returns a valid program
  2. define an environmental variable KALIGNDIR which points to a 
     directory containing the 'kalign' app:
   In bash 
	export KALIGNDIR=/home/progs/kalign   or
   In csh/tcsh
        setenv KALIGNDIR /home/progs/kalign

  3. include a definition of an environmental variable KALIGNDIR 
      in every script that will
     BEGIN {$ENV{KALIGNDIR} = '/home/progs/kalign'; }
     use Bio::Tools::Run::Alignment::Kalign;
Methods
BEGIN Code
program_nameDescriptionCode
program_dirDescriptionCode
newDescriptionCode
AUTOLOAD
No description
Code
error_stringDescriptionCode
versionDescriptionCode
runDescriptionCode
alignDescriptionCode
_runDescriptionCode
_setinputDescriptionCode
_setparamsDescriptionCode
aformatDescriptionCode
Methods description
program_namecode    nextTop
 Title   : program_name
 Usage   : $factory->program_name()
 Function: holds the program name
 Returns:  string
 Args    : None
program_dircodeprevnextTop
 Title   : program_dir
 Usage   : $factory->program_dir(@params)
 Function: returns the program directory, obtained from ENV variable.
 Returns:  string
 Args    :
newcodeprevnextTop
 Title   : new
 Usage   : my $kalign = Bio::Tools::Run::Alignment::Kalign->new();
 Function: Constructor
 Returns : Bio::Tools::Run::Alignment::Kalign
 Args    : -outfile_name => $outname
error_stringcodeprevnextTop
 Title   : error_string
 Usage   : $obj->error_string($newval)
 Function: Where the output from the last analysus run is stored.
 Returns : value of error_string
 Args    : newvalue (optional)
versioncodeprevnextTop
 Title   : version
 Usage   : exit if $prog->version() < 2
 Function: Determine the version number of the program
 Example :
 Returns : float or undef
 Args    : none
runcodeprevnextTop
 Title   : run
 Usage   : my $output = $application->run(\@seqs);
 Function: Generic run of an application
 Returns : Bio::SimpleAlign object
 Args    : Arrayref of Bio::PrimarySeqI objects or
           a filename to run on
aligncodeprevnextTop
 Title   : align
 Usage   :
	$inputfilename = 't/data/cysprot.fa';
	$aln = $factory->align($inputfilename);
or
	$seq_array_ref = \@seq_array; 
        # @seq_array is array of Seq objs
	$aln = $factory->align($seq_array_ref);
 Function: Perform a multiple sequence alignment
 Returns : Reference to a SimpleAlign object containing the
           sequence alignment.
 Args    : Name of a file containing a set of unaligned fasta sequences
           or else an array of references to Bio::Seq objects.

 Throws an exception if argument is not either a string (eg a
 filename) or a reference to an array of Bio::Seq objects.  If
 argument is string, throws exception if file corresponding to string
 name can not be found. If argument is Bio::Seq array, throws
 exception if less than two sequence objects are in array.
_runcodeprevnextTop
 Title   :  _run
 Usage   :  Internal function, not to be called directly	
 Function:  makes actual system call to kalign program
 Example :
 Returns : nothing; kalign output is written to a
           temporary file OR specified output file
 Args    : Name of a file containing a set of unaligned fasta sequences
           and hash of parameters to be passed to kalign
_setinputcodeprevnextTop
 Title   :  _setinput
 Usage   :  Internal function, not to be called directly	
 Function:  Create input file for kalign program
 Example :
 Returns : name of file containing kalign data input AND
 Args    : Arrayref of Seqs or input file name
_setparamscodeprevnextTop
 Title   :  _setparams
 Usage   :  Internal function, not to be called directly	
 Function:  Create parameter inputs for kalign program
 Example :
 Returns : parameter string to be passed to kalign
           during align or profile_align
 Args    : name of calling object
aformatcodeprevnextTop
 Title   : aformat
 Usage   : my $alignmentformat = $self->aformat();
 Function: Get/Set alignment format
 Returns : string
 Args    : string
Methods code
BEGINTop
BEGIN {
    %DEFAULTS = ( 'AFORMAT' => 'fasta' );
    @KALIGN_PARAMS = qw(IN OUT GAPOPEN GAPEXTENSION TERMINAL_GAP_EXTENSION_PENALTY MATRIX_BONUS 
                        SORT FEATURE DISTANCE TREE ZCUTOFF FORMAT 
			MAXMB MAXHOURS MAXITERS);
    @KALIGN_SWITCHES = qw(QUIET);

# Authorize attribute fields
    foreach my $attr ( @KALIGN_PARAMS, @KALIGN_SWITCHES ) {
	$OK_FIELD{$attr}++;
}
program_namedescriptionprevnextTop
sub program_name {
        return 'kalign';
}
program_dirdescriptionprevnextTop
sub program_dir {
        return Bio::Root::IO->catfile($ENV{KALIGNDIR}) if $ENV{KALIGNDIR};
}
newdescriptionprevnextTop
sub new {
    my ($class,@args) = @_;
    my( @kalign_args, @obj_args);
    while( my $arg = shift @args ) {
	if( $arg =~ /^-/ ) {
	    push @obj_args, $arg, shift @args;
	} else {
	    push @kalign_args,$arg, shift @args;
	}
    }
    my $self = $class->SUPER::new(@obj_args);
    
    my ($on) = $self->_rearrange([qw(OUTFILE_NAME)],@obj_args);
    
    $self->outfile_name($on || '');
    my ($attr, $value);    
    # FIXME: only tested with fasta output format right now...
    $self->aformat($DEFAULTS{'AFORMAT'});

    while ( @kalign_args)  {
	$attr =   shift @kalign_args;
	$value =  shift @kalign_args;
	next if( $attr =~ /^-/); # don't want named parameters
	$self->$attr($value);
    }
    
    if( defined $self->out ) {
	$self->outfile_name($self->out);
    }
    return $self;
}
AUTOLOADdescriptionprevnextTop
sub AUTOLOAD {
    my $self = shift;
    my $attr = $AUTOLOAD;
    $attr =~ s/.*:://;
    $attr = uc $attr;
    # aliasing
    $self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};

    $self->{$attr} = shift if @_;
    return $self->{$attr};
}
error_stringdescriptionprevnextTop
sub error_string {
   my ($self,$value) = @_;
   if( defined $value) {
      $self->{'error_string'} = $value;
    }
    return $self->{'error_string'};
}
versiondescriptionprevnextTop
sub version {
    my ($self) = @_;
    my $exe;
    # Kalign version 2.01, Copyright (C) 2004, 2005, 2006 Timo Lassmann
    return undef unless $exe = $self->executable;
    my $string = `$exe 2>&1` ;
    $string =~ /Kalign\s+version\s+(\d+\.\d+)/m;
    return $1 || undef;
}
rundescriptionprevnextTop
sub run {
    my $self = shift;
    return $self->align(shift);
}
aligndescriptionprevnextTop
sub align {
    my ($self,$input) = @_;
    # Create input file pointer
    $self->io->_io_cleanup();
    my $infilename;
    if( defined $input ) {
	$infilename = $self->_setinput($input);
    } elsif( defined $self->in ) {
	$infilename = $self->_setinput($self->in);
    } else {
	$self->throw("No inputdata provided\n");
    }
    if (! $infilename) {
	$self->throw("Bad input data or less than 2 sequences in $input !");
    }

    my $param_string = $self->_setparams();

    # run kalign
    return &_run($self, $infilename, $param_string);
}
_rundescriptionprevnextTop
sub _run {
    my ($self,$infilename,$params) = @_;
    my $commandstring = $self->executable." -in $infilename $params";
    
    $self->debug( "kalign command = $commandstring\n ");

    my $status = system($commandstring);
    my $outfile = $self->outfile_name(); 
    if( !-e $outfile || -z $outfile ) {
	$self->warn( "Kalign call crashed: $? [command $commandstring]\n");
	return undef;
    }

    my $in  = Bio::AlignIO->new('-file'   => $outfile, 
				'-format' => $self->aformat);
    my $aln = $in->next_aln();
    return $aln;
}
_setinputdescriptionprevnextTop
sub _setinput {
    my ($self,$input) = @_;
    my ($infilename, $seq, $temp, $tfh);
    if (! ref $input) {
	# check that file exists or throw
	$infilename = $input;
	unless (-e $input) {return 0;}
	# let's peek and guess
	open(IN,$infilename) || $self->throw("Cannot open $infilename");
	my $header;
	while( defined ($header = <IN>) ) {
	    last if $header !~ /^\s+$/;
	}
	close(IN);
	if ( $header !~ /^>\s*\S+/ ){
	    $self->throw("Need to provide a FASTA format file to kalign!");
	} 
	return ($infilename);
    } elsif (ref($input) =~ /ARRAY/i ) { #  $input may be an
	#  array of BioSeq objects...
        #  Open temporary file for both reading & writing of array
	($tfh,$infilename) = $self->io->tempfile();
	if( ! ref($input->[0]) ) {
	    $self->warn("passed an array ref which did not contain objects to _setinput");
	    return undef;
	} elsif( $input->[0]->isa('Bio::PrimarySeqI') ) {		
	    $temp =  Bio::SeqIO->new('-fh' => $tfh,
				     '-format' => 'fasta');
	    my $ct = 1;
	    foreach $seq (@$input) {
		return 0 unless ( ref($seq) && 
				  $seq->isa("Bio::PrimarySeqI") );
		if( ! defined $seq->display_id ||
		    $seq->display_id =~ /^\s+$/) {
		    $seq->display_id( "Seq".$ct++);
		} 
		$temp->write_seq($seq);
	    }
	    $temp->close();
	    undef $temp;
	    close($tfh);
	    $tfh = undef;
	} else { 
	    $self->warn( "got an array ref with 1st entry ".
			 $input->[0].
			 " and don't know what to do with it\n");
	}
	return ($infilename);
    } else { 
	$self->warn("Got $input and don't know what to do with it\n");
    }
    return 0;
}
_setparamsdescriptionprevnextTop
sub _setparams {
    my ($self) = @_;
    my ($attr, $value,$param_string);
    $param_string = '';
    my $laststr;
    for  $attr ( @KALIGN_PARAMS ) {
	$value = $self->$attr();
	next unless (defined $value);	
	my $attr_key = lc $attr;
        $attr_key = ' -'.$attr_key;
        $param_string .= $attr_key .' '.$value;

    }
    for  $attr ( @KALIGN_SWITCHES) {
 	$value = $self->$attr();
 	next unless ($value);
 	my $attr_key = lc $attr; #put switches in format expected by tcoffee
 	$attr_key = ' -'.$attr_key;
 	$param_string .= $attr_key ;
    }

    # Set default output file if no explicit output file selected
    unless ($self->outfile_name ) {	
	my ($tfh, $outfile) = $self->io->tempfile(-dir=>$self->tempdir());
	close($tfh);
	undef $tfh;
	$self->outfile_name($outfile);
    }
    $param_string .= " -out ".$self->outfile_name;
    
    if ($self->quiet() || $self->verbose < 0) { 
	$param_string .= ' 2> /dev/null';
    }
    return $param_string;
}
aformatdescriptionprevnextTop
sub aformat {
    my $self = shift;
    $self->{'_aformat'} = shift if @_;
    return $self->{'_aformat'};
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists
Support Top
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the web:
 http://redmine.open-bio.org/projects/bioperl/
AUTHOR - Albert VilellaTop
Email idontlikespam@hotmail.com
APPENDIXTop
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
Bio::Tools::Run::BaseWrapper methodsTop
no_param_checksTop
 Title   : no_param_checks
 Usage   : $obj->no_param_checks($newval)
 Function: Boolean flag as to whether or not we should
           trust the sanity checks for parameter values  
 Returns : value of no_param_checks
 Args    : newvalue (optional)
save_tempfilesTop
 Title   : save_tempfiles
 Usage   : $obj->save_tempfiles($newval)
 Function: 
 Returns : value of save_tempfiles
 Args    : newvalue (optional)
outfile_nameTop
 Title   : outfile_name
 Usage   : my $outfile = $kalign->outfile_name();
 Function: Get/Set the name of the output file for this run
           (if you wanted to do something special)
 Returns : string
 Args    : [optional] string to set value to
tempdirTop
 Title   : tempdir
 Usage   : my $tmpdir = $self->tempdir();
 Function: Retrieve a temporary directory name (which is created)
 Returns : string which is the name of the temporary directory
 Args    : none
cleanupTop
 Title   : cleanup
 Usage   : $kalign->cleanup();
 Function: Will cleanup the tempdir directory
 Returns : none
 Args    : none
ioTop
 Title   : io
 Usage   : $obj->io($newval)
 Function:  Gets a Bio::Root::IO object
 Returns : Bio::Root::IO
 Args    : none