Toolbar

Summary

Bio::Tools::Run::Phylo::QuickTree - Wrapper for rapid reconstruction of
phylogenies using QuickTree

Package variables

Included modules

Inherit

Bio::Tools::Run::WrapperBase

Synopsis

  use Bio::Tools::Run::Phylo::QuickTree;

  #  Make a QuickTree factory
  @params = ();
  $factory = Bio::Tools::Run::Phylo::QuickTree->new(@params);

  #  Pass the factory an alignment	
  $inputfilename = 't/data/cysprot.stockholm';
  $tree = $factory->run($inputfilename); # $tree is a Bio::Tree::Tree object.
  # or get a Bio::Align::AlignI (SimpleAlign) object from somewhere
  $tree = $factory->run($aln);

Description

This is a wrapper for running the QuickTree application by Kevin Howe. You
can download it here: http://www.sanger.ac.uk/Software/analysis/quicktree/
Currently only input with alignments and output of trees is supported. (Ie.
no support for distance matrix in/out.)
You will need to enable this QuickTree wrapper to find the quicktree program.
This can be done in (at least) three ways:

 1. Make sure the QuickTree executable is in your path.
 2. Define an environmental variable QUICKTREEDIR which is a 
    directory which contains the 'quicktree' application:
    In bash:

    export QUICKTREEDIR=/home/username/quicktree_1.1/bin

    In csh/tcsh:

    setenv QUICKTREEDIR /home/username/quicktree_1.1/bin

 3. Include a definition of an environmental variable QUICKTREEDIR in
    every script that will use this QuickTree wrapper module, e.g.:

    BEGIN { $ENV{QUICKTREEDIR} = '/home/username/quicktree_1.1/bin' }
    use Bio::Tools::Run::Phylo::QuickTree;

Methods

Methods description

 Title   : program_name
 Usage   : $factory>program_name()
 Function: holds the program name
 Returns : string
 Args    : None

 Title   : program_dir
 Usage   : $factory->program_dir(@params)
 Function: returns the program directory, obtained from ENV variable.
 Returns : string
 Args    : None

 Title   : new
 Usage   : $factory = Bio::Tools::Run::Phylo::QuickTree->new(@params)
 Function: creates a new QuickTree factory
 Returns : Bio::Tools::Run::Phylo::QuickTree
 Args    : Optionally, provide any of the following (default in []):
           -upgma  => boolean # Use the UPGMA method to construct the tree [0]
           -kimura => boolean # Use the kimura translation for pairwise
                              # distances [0]
           -boot   => int     # Calculate bootstrap values with n iterations [0]

 Title   : upgma
 Usage   : $factory->upgma(1);
 Function: Choose to use the UPGMA method to construct the tree.
 Returns : boolean (default 0)
 Args    : None to get, boolean to set.

 Title   : kimura
 Usage   : $factory->kimura(1);
 Function: Choose to use the kimura translation for pairwise distances.
 Returns : boolean (default 0)
 Args    : None to get, boolean to set.

 Title   : boot
 Usage   : $factory->boot(100);
 Function: Choose to calculate bootstrap values with the supplied number of
           iterations.
 Returns : int (default 0)
 Args    : None to get, int to set.

 Title   : run
 Usage   : $factory->run($stockholm_file);
           $factory->run($align_object);
 Function: Runs QuickTree to generate a tree 
 Returns : Bio::Tree::Tree object
 Args    : file name for your input alignment in stockholm format, OR
           Bio::Align::AlignI compliant object (eg. Bio::SimpleAlign).

 Title   : _setparams
 Usage   : Internal function, not to be called directly
 Function: Creates a string of params to be used in the command string
 Returns : string of params
 Args    : none

 Title   : _writeAlignFile
 Usage   : obj->_writeAlignFile($seq)
 Function: Internal(not to be used directly)
 Returns : filename
 Args    : Bio::Align::AlignI

Methods code

sub program_name {

    return $PROGRAM_NAME;

}

sub program_dir {

    return $PROGRAM_DIR;

}

sub new {

    my ($class, @args) = @_;
    my $self = $class->SUPER::new(@args);
    
    # for consistency with other run modules, allow params to be dashless
    my %args = @args;
    while (my ($key, $val) = each %args) {
        if ($key !~ /^-/) {
            delete $args{$key};
            $args{'-'.$key} = $val;
        }
    }
    
    my ($upgma, $kimura, $boot) = $self->_rearrange([qw(UPGMA
                                                        KIMURA
                                                        BOOT)], %args);
    
    $self->upgma(1) if $upgma;
    $self->kimura(1) if $kimura;
    $self->boot($boot) if $boot;
    
    return $self;

}

sub upgma {

    my ($self, $bool) = @_;
    if (defined ($bool)) {
        $self->{upgma} = $bool;
    }
    return $self->{upgma} || 0;

}

sub kimura {

    my ($self, $bool) = @_;
    if (defined ($bool)) {
        $self->{kimura} = $bool;
    }
    return $self->{kimura} || 0;

}

sub boot {

    my ($self, $int) = @_;
    if (defined ($int)) {
        $self->{boot} = $int;
    }
    return $self->{boot} || 0;

}

sub run {

    my ($self, $in) = @_;

    if (ref $in && $in->isa("Bio::Align::AlignI")) {
        $in = $self->_writeAlignFile($in);
    }
    elsif (! -e $in) {
        $self->throw("When not supplying a Bio::Align::AlignI object, you must supply a readable filename");
    }
    
    return $self->_run($in);

}

sub _run {

    my ($self, $file)= @_;
    
    my $exe = $self->executable || return;
    my $param_str = $self->arguments." ".$self->_setparams;
    my $command = $exe." $param_str ".$file;
    
    $self->debug("QuickTree command = $command");
    
    open(my $result, "$command |") || $self->throw("QuickTree call ($command) crashed: $?");
    my $treeio = Bio::TreeIO->new(-format => 'nhx', -fh => $result);
    my $tree = $treeio->next_tree;
    close($result);
    
    # if bootstraps were enabled, the bootstraps are the ids; convert to
    # bootstrap and no id
    if ($self->boot) {
        my @nodes = $tree->get_nodes;
        my %non_internal = map { $_ => 1 } ($tree->get_leaf_nodes, $tree->get_root_node);
        foreach my $node (@nodes) {
            next if exists $non_internal{$node};
            $node->bootstrap && next; # protect ourselves incase the parser improves
            $node->bootstrap($node->id);
            $node->id('');
        }
    }
    
    return $tree;

}

sub _setparams {

    my $self = shift;
    my $param_string = '-in a -out t';
    
    $param_string .= ' -upgma' if $self->upgma;
    $param_string .= ' -kimura' if $self->kimura;
    $param_string .= ' -boot '.$self->boot if $self->boot;
    
    return $param_string;

}

sub _writeAlignFile {

    my ($self, $align) = @_;
    
    my ($tfh, $tempfile) = $self->io->tempfile(-dir=>$self->tempdir);
    
    my $out = Bio::AlignIO->new('-fh'     => $tfh, 
				'-format' => 'stockholm');
    $out->write_aln($align);
    $out->close();
    $out = undef;
    close($tfh);
    undef $tfh;
    return $tempfile;
}

1;

}

General documentation

User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.

Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:

  http://redmine.open-bio.org/projects/bioperl/

Email bix@sendu.me.uk

The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _