Bio::Tools Genewise
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Summary
Bio::Tools::Genewise - Results of one Genewise run
Package variables
No package variables defined.
Included modules
Bio::Root::IO
Bio::Root::Root
Bio::SeqFeature::FeaturePair
Bio::SeqFeature::Gene::Exon
Bio::SeqFeature::Gene::GeneStructure
Bio::SeqFeature::Gene::Transcript
Bio::SeqFeature::Generic
Bio::Tools::AnalysisResult
Symbol
Inherit
Bio::Root::IO Bio::Root::Root
Synopsis
  use Bio::Tools::Genewise;
  my $gw = Bio::Tools::Genewise(-file=>"genewise.out");

  while (my $gene = $gw->next_prediction){
    my @transcripts = $gene->transcripts;
      foreach my $t(@transcripts){
        my @exons =  $t->exons;
        foreach my $e(@exons){
            print $e->start." ".$e->end."\n";
        }
      }
  }
Description
This is the parser for the output of Genewise. It takes either a file handle or
a file name and returns a Bio::SeqFeature::Gene::GeneStructure object.
Methods
newDescriptionCode
_get_strandDescriptionCode
_score
No description
Code
_prot_idDescriptionCode
_target_idDescriptionCode
next_predictionDescriptionCode
Methods description
newcode    nextTop
 Title   : new
 Usage   : $obj->new(-file=>"genewise.out");
           $obj->new(-fh=>\*GW);
 Function: Constructor for genewise wrapper. Takes either a file or filehandle
 Example :
 Returns : Bio::Tools::Genewise
_get_strandcodeprevnextTop
 Title   : _get_strand
 Usage   : $obj->_get_strand
 Function: takes start and end values, swap them if start>end and returns end
 Example :
 Returns :$start,$end,$strand
_prot_idcodeprevnextTop
 Title   : _prot_id
 Usage   : $obj->_prot_id
 Function: get/set for protein id 
 Example :
 Returns :a protein id
_target_idcodeprevnextTop
 Title   : _target_id
 Usage   : $obj->_target_id
 Function: get/set for genomic sequence id
 Example :
 Returns :a target id
next_predictioncodeprevnextTop
 Title   : next_prediction
 Usage   : while($gene = $genewise->next_prediction()) {
                  # do something
           }
 Function: Returns the gene structure prediction of the Genewise result
           file. Call this method repeatedly until FALSE is returned.

 Example :
 Returns : a Bio::SeqFeature::Gene::GeneStructure object
 Args    :
Methods code
newdescriptionprevnextTop
sub new {
  my($class,@args) = @_;
  my $self = $class->SUPER::new(@args);
  $self->_initialize_io(@args);
  return $self;
}
_get_stranddescriptionprevnextTop
sub _get_strand {
  my ($self,$start,$end) = @_;
  $start || $self->throw("Need a start");
  $end   || $self->throw("Need an end");
  my $strand;
  if ($start > $end) {
    my $tmp = $start;
    $start = $end;
    $end = $tmp;
    $strand = -1;
  }
  else {
    $strand = 1;
  }
  return ($start,$end,$strand);
}
_scoredescriptionprevnextTop
sub _score {
  my ($self,$val) = @_;
  if($val){
    $self->{'_score'} = $val;
  }
  return $self->{'_score'};
}
_prot_iddescriptionprevnextTop
sub _prot_id {
  my ($self,$val) = @_;
  if($val){
    $self->{'_prot_id'} = $val;
  }
  return $self->{'_prot_id'};
}
_target_iddescriptionprevnextTop
sub _target_id {
  my ($self,$val) = @_;
  if($val){
    $self->{'_target_id'} = $val;
  }
  return $self->{'_target_id'};
}
next_predictiondescriptionprevnextTop
sub next_prediction {
    my ($self) = @_;

    my $genes = new Bio::SeqFeature::Gene::GeneStructure(-source => $Srctag);
    my $transcript = new Bio::SeqFeature::Gene::Transcript(-source => $Srctag);

    local ($/) = "//";
    my $score;
    my $prot_id;
    my $target_id;
    while ( defined($_ = $self->_readline) ) {
	$self->debug( $_ ) if( $self->verbose > 0);
        ($score) = $_=~m/Score\s+(\d+[\.][\d]+)/;
        $self->_score($score) unless defined $self->_score;
        ($prot_id) = $_=~m/Query protein:\s+(\S+)/;
        $self->_prot_id($prot_id) unless defined $self->_prot_id;
        ($target_id) = $_=~m/Target Sequence\s+(\S+)/;	
        $self->_target_id($target_id) unless defined $self->_target_id;
        next unless /Gene\s+\d+\n/;

        #grab exon + supporting feature info
my @exons; unless ( @exons = $_ =~ m/(Exon .+\s+Supporting .+)/g ) { @exons = $_ =~ m/(Exon .+\s+)/g; } my $nbr = 1; #loop through each exon-supporting feature pair
foreach my $e (@exons){ my ($e_start,$e_end,$phase) = $e =~ m/Exon\s+(\d+)\s+(\d+)\s+phase\s+(\d+)/; my $e_strand; ($e_start,$e_end,$e_strand) = $self->_get_strand($e_start,$e_end); $transcript->strand($e_strand) unless $transcript->strand != 0; my $exon = new Bio::SeqFeature::Gene::Exon (-seq_id=>$self->_target_id, -source => $Srctag, -start=>$e_start, -end=>$e_end, #-frame => $phase,
-strand=>$e_strand); $exon->add_tag_value('phase',$phase); if( $self->_prot_id ) { $exon->add_tag_value('Sequence',"Protein:".$self->_prot_id); } $exon->add_tag_value("Exon",$nbr++); if( $e =~ m/Supporting\s+(\d+)\s+(\d+)\s+(\d+)\s+(\d+)/) { my ($geno_start,$geno_end, $prot_start, $prot_end) = ($1,$2,$3,$4); my $prot_strand; ($prot_start,$prot_end, $prot_strand) = $self->_get_strand($prot_start,$prot_end); my $pf = new Bio::SeqFeature::Generic ( -start => $prot_start, -end => $prot_end, -seq_id => $self->_prot_id, -score => $self->_score, -strand => $prot_strand, -source => $Srctag, -primary=> 'supporting_protein_feature', ); my $geno_strand; ($geno_start,$geno_end, $geno_strand) = $self->_get_strand($geno_start,$geno_end); my $gf = new Bio::SeqFeature::Generic ( -start => $geno_start, -end => $geno_end, -seq_id => $self->_target_id, -score => $self->_score, -strand => $geno_strand, -source => $Srctag, -primary => 'supporting_genomic_feature', ); my $fp = new Bio::SeqFeature::FeaturePair(-feature1=>$gf, -feature2=>$pf); $exon->add_tag_value( 'supporting_feature' => $fp ); } $transcript->add_exon($exon); } $transcript->seq_id($self->_target_id); $genes->add_transcript($transcript); $genes->seq_id($self->_target_id); return $genes; }
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
  bioperl-l@bioperl.org          - General discussion
  http://bio.perl.org/MailList.html             - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
  bioperl-bugs@bio.perl.org
  http://bugzilla.bioperl.org/
AUTHOR - Fugu Team Top
 Email: fugui@worf.fugu-sg.org
APPENDIXTop
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
scoreTop
 Title   : score
 Usage   : $obj->score
 Function: get/set for score info
 Example :
 Returns : a score value