Summary

Bio::DB::GFF::RelSegment -- Sequence segment with relative coordinate support

Package variables

Included modules

Inherit

Bio::DB::GFF::Segment

Synopsis

See Bio::DB::GFF.

Description

Bio::DB::GFF::RelSegment is a stretch of sequence that can handle
relative coordinate addressing. It inherits from
Bio::DB::GFF::Segment, and is the base class for
Bio::DB::GFF::Feature.
In addition to the source sequence, a relative segment has a
"reference sequence", which is used as the basis for its coordinate
system. The reference sequence can be changed at will, allowing you
freedom to change the "frame of reference" for features contained
within the segment. For example, by setting a segment's reference
sequence to the beginning of a gene, you can view all other features
in gene-relative coordinates.
The reference sequence and the source sequence must be on the same
physical stretch of DNA, naturally. However, they do not have to be
on the same strand. The strandedness of the reference sequence
determines whether coordinates increase to the right or the left.
Generally, you will not create or manipulate Bio::DB::GFF::RelSeg0ment
objects directly, but use those that are returned by the Bio::DB::GFF
module. To understand how relative coordinates work, consider the following
example from the C. elegans database. First we create the appropriate
GFF accessor object (the factory):

   my $db = Bio::DB::GFF->new(-dsn => 'dbi:mysql:elegans',
                              -adaptor=>'dbi:mysqlopt');

Now we fetch out a segment based on cosmid clone ZK909:

  my $seg = $db->segment('ZK909');

If we call the segment's refseq() method, we see that the base of the
coordinate system is the sequence "ZK154", and that its start and
stop positions are 1 and the length of the cosmid:

  print $seg->refseq;
  => ZK909

  print $seg->start,' - ',$seg->stop;
  => 1 - 33782

As a convenience, the "" operator is overloaded in this class, to give
the reference sequence, and start and stop positions:

  print $seg;
  => ZK909:1,33782

Internally, Bio::DB::GFF::RelSegment has looked up the absolute
coordinates of this segment and maintains the source sequence and the
absolute coordinates relative to the source sequence. We can see this
information using sourceseq() (inherited from Bio::DB::GFF::Segment)
and the abs_start() and abs_end() methods:

  print $seg->sourceseq;
  => CHROMOSOME_I

  print $seg->abs_start,' - ',$seg->abs_end;
  => 14839545 - 14873326

We can also put the segment into absolute mode, so that it behaves
like Bio::DB::Segment, and always represents coordinates on the source
sequence. This is done by passing a true value to the absolute()
method:

  $seq->absolute(1);
  print $seg;
  => CHROMOSOME_I:14839545,14873326

We can change the reference sequence at any time. One way is to call
the segment's ref() method, giving it the ID (and optionally the
class) of another landmark on the genome. For example, if we know
that cosmid ZK337 is adjacent to ZK909, then we can view ZK909 in
ZK337-relative coordinates:

  $seg->refseq('ZK337');
  print $seg;
  => ZK337:-33670,111

We can call the segment's features() method in order to get the list
of contigs that overlap this segment (in the C. elegans database,
contigs have feature type "Sequence:Link"):

  @links = $seg->features('Sequence:Link');

We can now set the reference sequence to the first of these contigs like so:

  $seg->refseq($links[0]);
  print $seg;
  => Sequence:Link(LINK_Y95D11A):3997326,4031107

Methods

Methods description

 Title   : new
 Usage   : $s = Bio::DB::GFF::RelSegment->new(@args)
 Function: create a new relative segment
 Returns : a new Bio::DB::GFF::RelSegment object
 Args    : see below
 Status  : Public

 -factory      a Bio::DB::GFF::Adaptor to use for database access
 -seq          ID of the source sequence
 -class        class of the source sequence
 -start        start of the desired segment relative to source sequence
 -stop         stop of the desired segment relative to source sequence
 -ref          ID of the reference sequence
 -refclass     class of the reference sequence
 -offset       0-based offset from source sequence to start of segment
 -length       length of desired segment
 -absolute, -force_absolute
               use absolute coordinates, rather than coordinates relative
               to the start of self or the reference sequence

 Title   : refseq
 Usage   : $ref = $s->refseq([$newseq] [,$newseqclass])
 Function: get/set reference sequence
 Returns : current reference sequence
 Args    : new reference sequence and class (optional)
 Status  : Public

 Title   : abs_low
 Usage   : $s->abs_low
 Function: the absolute lowest coordinate of the segment
 Returns : an integer
 Args    : none
 Status  : Public

 Title   : abs_high
 Usage   : $s->abs_high
 Function: the absolute highest coordinate of the segment
 Returns : an integer
 Args    : none
 Status  : Public

 Title   : asString
 Usage   : $s->asString
 Function: human-readable representation of the segment
 Returns : a string
 Args    : none
 Status  : Public

  refseq:start,stop

 Title   : absolute
 Usage   : $abs = $s->absolute([$abs])
 Function: get/set absolute coordinates
 Returns : a boolean flag
 Args    : new setting for flag (optional)
 Status  : Public

 Title   : features
 Usage   : @features = $s->features(@args)
 Function: get features that overlap this segment
 Returns : a list of Bio::DB::GFF::Feature objects
 Args    : see below
 Status  : Public

 @f = $s->features('exon:curated');           # all curated exons
 @f = $s->features('exon:curated','intron');  # curated exons and all introns
 @f = $s->features('similarity:.*EST.*');     # all similarities
                                              # having something to do
                                              # with ESTs

  -types      an array reference to type names in the format
	      "method:source"

  -merge     Whether to apply aggregators to the generated features (default yes)

  -rare      Turn on an optimization suitable for a relatively rare feature type,
             where it will be faster to filter by feature type first
             and then by position, rather than vice versa.

  -attributes a hashref containing a set of attributes to match

  -range_type One of 'overlapping', 'contains', or 'contained_in'

  -iterator  Whether to return an iterator across the features.

  -binsize   A true value will create a set of artificial features whose
             start and stop positions indicate bins of the given size, and
             whose scores are the number of features in the bin.  The
             class and method of the feature will be set to "bin",
             its source to "method:source", and its group to "bin:method:source".
             This is a handy way of generating histograms of feature density.

  -attributes => { Gene => 'abc-1',
                   Note => 'confirmed' }

 Title   : feature_count
 Usage   : $seq->feature_count()
 Function: Return the number of SeqFeatures attached to a sequence
 Returns : integer representing the number of SeqFeatures
 Args    : none

 Title   : features
 Usage   : $stream = $s->get_feature_stream(@args)
 Function: get a stream of features that overlap this segment
 Returns : a Bio::SeqIO::Stream-compliant stream
 Args    : see below
 Status  : Public

 $stream = $s->get_feature_stream('exon');
 while (my $exon = $stream->next_seq) {
    print $exon->start,"\n";
 }

 Title   : contained_features
 Usage   : @features = $s->contained_features(@args)
 Function: get features that are contained by this segment
 Returns : a list of Bio::DB::GFF::Feature objects
 Args    : see features()
 Status  : Public

 Title   : contained_in
 Usage   : @features = $s->contained_in(@args)
 Function: get features that contain this segment
 Returns : a list of Bio::DB::GFF::Feature objects
 Args    : see features()
 Status  : Public

 Title   : delete
 Usage   : $db->delete(@args)
 Function: delete features
 Returns : count of features deleted -- if available
 Args    : numerous, see below
 Status  : public

 -type,-types  Either a single scalar type to be deleted, or an
               reference to an array of types.

 -range_type   Control the range type of the deletion.  One of "overlaps" (default)
               "contains" or "contained_in"

  $segment->delete(-type=>['intron','repeat:repeatMasker']);  # remove all introns & repeats
  $segment->delete(-type=>['intron','repeat:repeatMasker']
		   -range_type => 'contains');                # remove all introns & repeats
                                                              # strictly contained in segment

 Title   : _process_feature_args
 Usage   : @args = $s->_process_feature_args(@args)
 Function: preprocess arguments passed to features, 
           contained_features, and overlapping_features
 Returns : a list of parsed arguents
 Args    : see feature()
 Status  : Internal

 Title   : types
 Usage   : @types = $s->types([-enumerate=>1])
 Function: list feature types that overlap this segment
 Returns : a list of Bio::DB::GFF::Typename objects or a hash
 Args    : see below
 Status  : Public

  %count = $s->types(-enumerate=>1);

 Title   : new_from_segment
 Usage   : $s = $segment->new_from_segment(@args)
 Function: create a new relative segment
 Returns : a new Bio::DB::GFF::RelSegment object
 Args    : see below
 Status  : Internal

 Title   : _abs2rel
 Usage   : @coords = $s->_abs2rel(@coords)
 Function: convert absolute coordinates into relative coordinates
 Returns : a list of relative coordinates
 Args    : a list of absolute coordinates
 Status  : Internal

 Title   : rel2abs
 Usage   : @coords = $s->rel2abs(@coords)
 Function: convert relative coordinates into absolute coordinates
 Returns : a list of absolute coordinates
 Args    : a list of relative coordinates
 Status  : Public

 Title   : abs2rel
 Usage   : @rel_coords = $s-abs2rel(@abs_coords)
 Function: convert absolute coordinates into relative coordinates
 Returns : a list of relative coordinates
 Args    : a list of absolute coordinates
 Status  : Public

Methods code

sub new {

  my $package = shift;
  my ($factory,$name,$start,$stop,$refseq,$class,$refclass,$offset,$length,$force_absolute,$nocheck) =
    rearrange([
	       'FACTORY',
	       [qw(NAME SEQ SEQUENCE SOURCESEQ)],
	       [qw(START BEGIN)],
	       [qw(STOP END)],
	       [qw(REFSEQ REF REFNAME)],
	       [qw(CLASS SEQCLASS)],
	       qw(REFCLASS),
	       [qw(OFFSET OFF)],
	       [qw(LENGTH LEN)],
	       [qw(ABSOLUTE)],
	       [qw(NOCHECK FORCE)],
	     ],@_);

  $package = ref $package if ref $package;
  $factory or $package->throw("new(): provide a -factory argument");

  # to allow people to use segments as sources
  if (ref($name) && $name->isa('Bio::DB::GFF::Segment')) {
    $start = 1              unless defined $start;
    $stop  = $name->length  unless defined $stop;
    return $name->subseq($start,$stop);
  }

  my @object_results;

  # support for Featname objects
  if (ref($name) && $name->can('class')) {
    $class = $name->class;
    $name  = $name->name;
  }
  # if the class of the landmark is not specified then default to 'Sequence'
  $class ||= eval{$factory->default_class} || 'Sequence';

  # confirm that indicated sequence is actually in the database!
  my @abscoords;

  # abscoords() will now return an array ref, each element of which is
  # ($absref,$absclass,$absstart,$absstop,$absstrand)

  if ($nocheck) {
    $force_absolute++;
    $start = 1;
  }

  if ($force_absolute && defined($start)) { # absolute position is given to us
    @abscoords = ([$name,$class,$start,$stop,'+']);
  } else {
    my $result = $factory->abscoords($name,$class,$force_absolute ? $name : ()) or return;
    @abscoords = @$result;
  }

  foreach (@abscoords) {
    my ($absref,$absclass,$absstart,$absstop,$absstrand,$sname) = @$_;
    $sname = $name unless defined $sname;
    my ($this_start,$this_stop,$this_length) = ($start,$stop,$length);

    # partially fill in object
    my $self = bless { factory => $factory },$package;

    $absstrand ||= '+';

    if ($absstart > $absstop) { # AAARGH!  DATA FORMAT ERROR!  FIX.
	($absstart,$absstop) = ($absstop,$absstart);
	$absstrand = $absstrand eq '+' ? '-' : '+';
    }

    # an explicit length overrides start and stop
    if (defined $offset) {
      warn "new(): bad idea to call new() with both a start and an offset"
	if defined $this_start;
      $this_start = $offset+1;
    }
    if (defined $this_length) {
      warn "new(): bad idea to call new() with both a stop and a length"
	if defined $this_stop;
      $this_stop = $this_start + $length - 1;
    }

    # this allows a SQL optimization way down deep
    $self->{whole}++ if $absref eq $sname and !defined($this_start) and !defined($this_stop);

    $this_start     = 1                    if !defined $this_start;
    $this_stop      = $absstop-$absstart+1 if !defined $this_stop;
    $this_length = $this_stop - $this_start + 1;

    # now offset to correct subsegment based on desired start and stop
    if ($force_absolute) {
      ($this_start,$this_stop) = ($absstart,$absstop);
      $self->absolute(1);
    } elsif ($absstrand eq '+') {
      $this_start =  $absstart   + $this_start - 1;
      $this_stop  =  $this_start + $this_length - 1;
    } else {
      $this_start =  $absstop - ($this_start - 1);
      $this_stop  =  $absstop - ($this_stop - 1);
    }

    # handle truncation in either direction
    # This only happens if the segment runs off the end of
    # the reference sequence
    if ($factory->strict_bounds_checking &&
	(($this_start < $absstart) || ($this_stop > $absstop))) {
      # return empty if we are completely off the end of the ref se
      next unless $this_start<=$absstop && $this_stop>=$absstart;
      if (my $a = $factory->abscoords($absref,'Sequence')) {
	my $refstart = $a->[0][2];
	my $refstop  = $a->[0][3];
	if ($this_start < $refstart) {
	  $this_start = $refstart;
	  $self->{truncated}{start}++;
	}
	if ($this_stop > $refstop) {
	  $this_stop = $absstop;
	  $self->{truncated}{stop}++;
	}
      }
    }

    @{$self}{qw(sourceseq start stop strand class)}
      = ($absref,$this_start,$this_stop,$absstrand,$absclass);

    # handle reference sequence
    if (defined $refseq) {
      $refclass = $refseq->class if $refseq->can('class');
      $refclass ||= 'Sequence';
      my ($refref,$refstart,$refstop,$refstrand) = $factory->abscoords($refseq,$refclass);
      unless ($refref eq $absref) {
	$self->error("reference sequence is on $refref but source sequence is on $absref");
	return;
      }
      $refstart = $refstop if $refstrand eq '-';
      @{$self}{qw(ref refstart refstrand)} = ($refseq,$refstart,$refstrand);
    } else {
      $absstart = $absstop if $absstrand eq '-';
      @{$self}{qw(ref refstart refstrand)} = ($sname,$absstart,$absstrand);
    }
    push @object_results,$self;
  }

  return wantarray ? @object_results : $object_results[0];

}

sub start {

  my $self = shift;
  return $self->strand < 0 ? $self->{stop} : $self->{start} if $self->absolute;
  $self->_abs2rel($self->{start});

}

sub end {

  my $self = shift;
  return $self->strand < 0 ? $self->{start} : $self->{stop} if $self->absolute;
  $self->_abs2rel($self->{stop});

}

sub length {

  my $self = shift;
  return unless defined $self->abs_end;
  abs($self->abs_end - $self->abs_start) + 1;

}

sub abs_start {

  my $self = shift;
  if ($self->absolute) {
    my ($a,$b) = ($self->SUPER::abs_start,$self->SUPER::abs_end);
    return ($a<$b) ? $a : $b;
  }
  else {
    return $self->SUPER::abs_start(@_);
  }

}

sub abs_end {

  my $self = shift;
  if ($self->absolute) {
    my ($a,$b) = ($self->SUPER::abs_start,$self->SUPER::abs_end);
    return ($a>$b) ? $a : $b;
  }

  else {
    return $self->SUPER::abs_end(@_);
  }

}

sub refseq {

  my $self = shift;
  my $g    = $self->{ref};
  if (@_) {
    my ($newref,$newclass);
    if (@_ == 2) {
      $newclass = shift;
      $newref   = shift;
    } else {
      $newref   = shift;
      $newclass = 'Sequence';
    }

    defined $newref or $self->throw('refseq() called with an undef reference sequence');

    # support for Featname objects
    $newclass = $newref->class if ref($newref) && $newref->can('class');

    # $self->throw("Cannot define a segment's reference sequence in terms of itself!")
    # if ref($newref) and overload::StrVal($newref) eq overload::StrVal($self);

    my ($refsource,undef,$refstart,$refstop,$refstrand);
    if ($newref->isa('Bio::DB::GFF::RelSegment')) {
      ($refsource,undef,$refstart,$refstop,$refstrand) =
	($newref->sourceseq,undef,$newref->abs_start,$newref->abs_end,$newref->abs_strand >= 0 ? '+' : '-');
    } else {
      my $coords = $self->factory->abscoords($newref,$newclass);
      foreach (@$coords) { # find the appropriate one
	($refsource,undef,$refstart,$refstop,$refstrand) = @$_;
	last if $refsource eq $self->{sourceseq};
      }
	
    }
    $self->throw("can't set reference sequence: $newref and $self are on different sequence segments")
      unless $refsource eq $self->{sourceseq};

    @{$self}{qw(ref refstart refstrand)} = ($newref,$refstart,$refstrand);
    $self->absolute(0);
  }
  return $self->absolute ? $self->sourceseq : $g;

}

sub abs_low {

  my $self = shift;
  my ($a,$b) = ($self->abs_start,$self->abs_end);
  return ($a<$b) ? $a : $b;

}

sub abs_high {

  my $self = shift;
  my ($a,$b) = ($self->abs_start,$self->abs_end);
  return ($a>$b) ? $a : $b;

}

sub asString {

  my $self = shift;
  return $self->SUPER::asString if $self->absolute;
  my $label = $self->{ref};
  my $start = $self->start || '';
  my $stop  = $self->stop  || '';
  if (ref($label) && overload::StrVal($self) eq overload::StrVal($label->ref)) {
    $label = $self->abs_ref;
    $start = $self->abs_start;
    $stop  = $self->abs_end;
  }
  return "$label:$start,$stop";

}

sub name {

 shift->asString

}

sub absolute {

  my $self = shift;
  my $g = $self->{absolute};
  $self->{absolute} = shift if @_;
  $g;

}

sub features {

  my $self = shift;
  my @args = $self->_process_feature_args(@_);
  return $self->factory->overlapping_features(@args);

}

sub feature_count {

     my $self = shift;
    my $ct = 0;
    my %type_counts = $self->types(-enumerate=>1);
    map { $ct += $_ } values %type_counts;
    $ct;

}

sub get_feature_stream {

  my $self = shift;
  my @args = defined($_[0]) && $_[0] =~ /^-/ ? (@_,-iterator=>1) : (-types=>\@_,-iterator=>1);
  $self->features(@args);

}

sub contained_features {

  my $self = shift;
  local $self->{whole} = 0;
  my @args = $self->_process_feature_args(@_);
  return $self->factory->contained_features(@args);

}

sub contained_in {

  my $self = shift;
  local $self->{whole} = 0;
  my @args = $self->_process_feature_args(@_);
  return $self->factory->contained_in(@args);

}

sub delete {

  my $self = shift;
  my ($type,$range_type) =
    rearrange([[qw(TYPE TYPES)],'RANGE_TYPE'],@_);
  my $types = $self->factory->parse_types($type);  # parse out list of types
  $range_type ||= 'overlaps';
  return $self->factory->_delete({
                                  segments   => [$self],
                                  types      => $types,
                                  range_type => $range_type
                                  });

}

sub _process_feature_args {

  my $self       = shift;

  my ($ref,$class,$start,$stop,$strand,$whole)
    = @{$self}{qw(sourceseq class start stop strand whole)};

  ($start,$stop) = ($stop,$start) if $strand eq '-';

  my @args = (-ref=>$ref,-class=>$class);

  # indicating that we are fetching the whole segment allows certain
  # SQL optimizations.
  push @args,(-start=>$start,-stop=>$stop) unless $whole;

  if (@_) {
    if ($_[0] =~ /^-/) {
      push @args,@_;
    } else {
      my @types = @_;
      push @args,-types=>\@types;
    }
  }
  push @args,-parent=>$self;
  @args;

}

sub types {

  my $self = shift;
  my ($ref,$class,$start,$stop,$strand) = @{$self}{qw(sourceseq class start stop strand)};
  ($start,$stop) = ($stop,$start) if $strand eq '-';

  my @args;
  if (@_ && $_[0] !~ /^-/) {
    @args = (-type =>\@ _)
  } else {
    @args = @_;
  }
  $self->factory->types(-ref  => $ref,
			-class => $class,
			-start=> $start,
			-stop => $stop,
			@args);

}

sub new_from_segment {

  my $package   = shift;
  $package      = ref $package if ref $package;
  my $segment   = shift;
  my $new = {};
  @{$new}{qw(factory sourceseq start stop strand class ref refstart refstrand)}
    = @{$segment}{qw(factory sourceseq start stop strand class ref refstart refstrand)};
  return bless $new,__PACKAGE__;

}

sub _abs2rel {

  my $self = shift;
  my @result;
  return unless defined $_[0];

  if ($self->absolute) {
    @result = @_;
  } else {
    my ($refstart,$refstrand) = @{$self}{qw(refstart refstrand)};
    @result = defined($refstrand) && $refstrand eq '-' ? map { $refstart - $_ + 1 } @_
                                                       : map { $_ - $refstart + 1 } @_;
  }
  # if called with a single argument, caller will expect a single scalar reply
  # not the size of the returned array!
  return $result[0] if @result == 1 and !wantarray;
  @result;

}

sub rel2abs {

  my $self = shift;
  my @result;

  if ($self->absolute) {
    @result = @_;
  } else {
    my ($abs_start,$abs_strand) = ($self->abs_start,$self->abs_strand);
    @result = $abs_strand < 0 ? map { $abs_start - $_ + 1 } @_
                              : map { $_ + $abs_start - 1 } @_;
  }
  # if called with a single argument, caller will expect a single scalar reply
  # not the size of the returned array!
  return $result[0] if @result == 1 and !wantarray;
  @result;

}

sub abs2rel {

  my $self = shift;
  my @result;

  if ($self->absolute) {
    @result = @_;
  } else {
    my ($abs_start,$abs_strand) = ($self->abs_start,$self->abs_strand);
    @result = $abs_strand < 0 ? map { $abs_start - $_ + 1 } @_
                              : map { $_ - $abs_start + 1 } @_;
  }
  # if called with a single argument, caller will expect a single scalar reply
  # not the size of the returned array!
  return $result[0] if @result == 1 and !wantarray;
  @result;

}

sub subseq {

  my $self = shift;
  my $obj  = $self->SUPER::subseq(@_);
  bless $obj,__PACKAGE__;    # always bless into the generic RelSegment package

}

sub strand {

  my $self = shift;
  if ($self->absolute) {
    return _to_strand($self->{strand});
  }
  return $self->stop <=> $self->start;

}

sub _to_strand {

  my $s = shift;
  return -1 if $s eq '-';
  return +1 if $s eq '+';
  return 0;

}

sub intersection {

  my $self     = shift;
  my (@ranges) = @_;
  unshift @ranges,$self if ref $self;
  $ranges[0]->isa('Bio::DB::GFF::RelSegment')
    or return $self->SUPER::intersection(@_);

  my $ref = $ranges[0]->abs_ref;
  my ($low,$high);
  foreach (@ranges) {
    return unless $_->can('abs_ref');
    $ref eq $_->abs_ref or return;
    $low  = $_->abs_low   if !defined($low)  or $low  < $_->abs_low;
    $high = $_->abs_high  if !defined($high) or $high > $_->abs_high;
  }
  return unless $low < $high;
  $self->new(-factory=> $self->factory,
	     -seq    => $ref,
	     -start  => $low,
	     -stop   => $high);

}

sub overlaps {

  my $self = shift;
  my($other,$so) = @_;
  return $self->SUPER::overlaps(@_) unless $other->isa('Bio::DB::GFF::RelSegment');
  return if $self->abs_ref ne $other->abs_ref;
  return if $self->abs_low  > $other->abs_high;
  return if $self->abs_high < $other->abs_low;
  1;

}

sub contains {

  my $self = shift;
  my($other,$so) = @_;
  return $self->SUPER::overlaps(@_) unless $other->isa('Bio::DB::GFF::RelSegment');
  return if $self->abs_ref ne $other->abs_ref;
  return unless $self->abs_low <= $other->abs_low;
  return unless $self->abs_high >= $other->abs_high;
  1;

}

sub union {

  my $self     = shift;
  my (@ranges) = @_;
  unshift @ranges,$self if ref $self;
  $ranges[0]->isa('Bio::DB::GFF::RelSegment')
    or return $self->SUPER::union(@_);

  my $ref = $ranges[0]->abs_ref;
  my ($low,$high);
  foreach (@ranges) {
    return unless $_->can('abs_ref');
    $ref eq $_->abs_ref or return;
    $low  = $_->abs_low  if !defined($low)  or $low  > $_->abs_low;
    $high = $_->abs_high if !defined($high) or $high < $_->abs_high;
  }
  $self->new(-factory=> $self->factory,
	     -seq    => $ref,
	     -start  => $low,
	     -stop   => $high);

}

General documentation

The remainder of this document describes the API for
Bio::DB::GFF::Segment.

 Title   : get_SeqFeatures
 Usage   :
 Function: returns the top level sequence features
 Returns : Bio::SeqFeatureI objects
 Args    : none

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : get_all_SeqFeatures
 Usage   :
 Function: returns all the sequence features
 Returns : Bio::SeqFeatureI objects
 Args    :

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : sub_SeqFeatures
 Usage   :
 Function:
 Example :
 Returns :
 Args    :

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : top_SeqFeatures
 Usage   :
 Function:
 Example :
 Returns :
 Args    :

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : all_SeqFeatures
 Usage   :
 Function:
 Example :
 Returns :
 Args    :

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : sub_SeqFeatures
 Usage   :
 Function:
 Example :
 Returns :
 Args    :

Alias for features(). Provided for Bio::SeqI compatibility.

 Title   : get_seq_stream
 Usage   : $stream = $s->get_seq_stream(@args)
 Function: get a stream of features that overlap this segment
 Returns : a Bio::SeqIO::Stream-compliant stream
 Args    : see below
 Status  : Public

This is the same as feature_stream(), and is provided for Bioperl
compatibility. Use like this:

 $stream = $s->get_seq_stream('exon');
 while (my $exon = $stream->next_seq) {
    print $exon->start,"\n";
 }

 Title   : overlapping_features
 Usage   : @features = $s->overlapping_features(@args)
 Function: get features that overlap this segment
 Returns : a list of Bio::DB::GFF::Feature objects
 Args    : see features()
 Status  : Public

This is an alias for the features() method, and takes the same
arguments.

The following are internal methods and should not be called directly.

The following Bio::RangeI methods are supported:
overlaps(), contains(), equals(),intersection(),union(),overlap_extent()

Schemas need some work.

Lincoln Stein <lstein@cshl.org>.
Copyright (c) 2001 Cold Spring Harbor Laboratory.
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.