Bio::Search::Hit GenericHit
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Summary
Bio::Search::Hit::GenericHit - A generic implementation of the Bio::Search::Hit::HitI interface
Package variables
No package variables defined.
Included modules
Bio::Root::Root
Bio::Search::Hit::HitI
Inherit
Bio::Root::Root Bio::Search::Hit::HitI
Synopsis
    use Bio::Search::Hit::GenericHit;
    my $hit = new Bio::Search::Hit::GenericHit(-algorithm => 'blastp');

    # typically one gets HitI objects from a SearchIO stream via a ResultI
    use Bio::SearchIO;
    my $parser = new Bio::SearchIO(-format => 'blast', -file => 'result.bls');

    my $result = $parser->next_result;
    my $hit    = $result->next_hit;
# TODO: Describe how to configure a SearchIO stream so that it generates
# GenericHit objects.
Description
This object handles the hit data from a Database Sequence Search such
as FASTA or BLAST.
Unless you're writing a parser, you won't ever need to create a
GenericHit or any other HitI-implementing object. If you use
the SearchIO system, HitI objects are created automatically from
a SearchIO stream which returns Bio::Search::Hit::HitI objects.
For documentation on what you can do with GenericHit (and other HitI
objects), please see the API documentation in
Bio::Search::Hit::HitI.
Methods
newDescriptionCode
add_hspDescriptionCode
nameDescriptionCode
accessionDescriptionCode
descriptionDescriptionCode
lengthDescriptionCode
algorithmDescriptionCode
raw_scoreDescriptionCode
scoreDescriptionCode
significanceDescriptionCode
bitsDescriptionCode
next_hspDescriptionCode
hspsDescriptionCode
num_hspsDescriptionCode
rewindDescriptionCode
ambiguous_alnDescriptionCode
overlapDescriptionCode
nDescriptionCode
pDescriptionCode
hspDescriptionCode
logical_lengthDescriptionCode
length_alnDescriptionCode
gapsDescriptionCode
matchesDescriptionCode
startDescriptionCode
endDescriptionCode
rangeDescriptionCode
frac_identicalDescriptionCode
frac_conservedDescriptionCode
frac_aligned_queryDescriptionCode
frac_aligned_hitDescriptionCode
num_unaligned_hitDescriptionCode
num_unaligned_queryDescriptionCode
seq_indsDescriptionCode
strandDescriptionCode
frameDescriptionCode
rankDescriptionCode
locusDescriptionCode
each_accession_numberDescriptionCode
tiled_hspsDescriptionCode
query_lengthDescriptionCode
Methods description
newcode    nextTop
 Title   : new
 Usage   : my $obj = new Bio::Search::Hit::GenericHit();
 Function: Builds a new Bio::Search::Hit::GenericHit object 
 Returns : Bio::Search::Hit::GenericHit
 Args    : -name         => Name of Hit (required)
           -description  => Description (optional)
           -accession    => Accession number (optional)
           -length       => Length of the Hit (optional)
           -score        => Raw Score for the Hit (optional)
           -bits         => Bit Score for the Hit (optional)
           -significance => Significance value for the Hit (optional)
           -algorithm    => Algorithm used (BLASTP, FASTX, etc...)
           -hsps         => Array ref of HSPs for this Hit. 
           -found_again  => boolean, true if hit appears in a 
                            "previously found" section of a PSI-Blast report.
add_hspcodeprevnextTop
 Title   : add_hsp
 Usage   : $hit->add_hsp($hsp)
 Function: Add a HSP to the collection of HSPs for a Hit
 Returns : number of HSPs in the Hit
 Args    : Bio::Search::HSP::HSPI object
namecodeprevnextTop
 Title   : name
 Usage   : $hit_name = $hit->name();
 Function: returns the name of the Hit sequence
 Returns : a scalar string
 Args    : [optional] scalar string to set the name
accessioncodeprevnextTop
 Title   : accession
 Usage   : $acc = $hit->accession();
 Function: Retrieve the accession (if available) for the hit
 Returns : a scalar string (empty string if not set)
 Args    : none
descriptioncodeprevnextTop
 Title   : description
 Usage   : $desc = $hit->description();
 Function: Retrieve the description for the hit
 Returns : a scalar string
 Args    : [optional] scalar string to set the descrition
lengthcodeprevnextTop
 Title   : length
 Usage   : my $len = $hit->length
 Function: Returns the length of the hit 
 Returns : integer
 Args    : [optional] integer to set the length
algorithmcodeprevnextTop
 Title   : algorithm
 Usage   : $alg = $hit->algorithm();
 Function: Gets the algorithm specification that was used to obtain the hit
           For BLAST, the algorithm denotes what type of sequence was aligned 
           against what (BLASTN: dna-dna, BLASTP prt-prt, BLASTX translated 
           dna-prt, TBLASTN prt-translated dna, TBLASTX translated 
           dna-translated dna).
 Returns : a scalar string 
 Args    : [optional] scalar string to set the algorithm
raw_scorecodeprevnextTop
 Title   : raw_score
 Usage   : $score = $hit->raw_score();
 Function: Gets the "raw score" generated by the algorithm.  What
           this score is exactly will vary from algorithm to algorithm,
           returning undef if unavailable.
 Returns : a scalar value
 Args    : [optional] scalar value to set the raw score
scorecodeprevnextTop
Equivalent to raw_score()
significancecodeprevnextTop
 Title   : significance
 Usage   : $significance = $hit->significance();
 Function: Used to obtain the E or P value of a hit, i.e. the probability that
           this particular hit was obtained purely by random chance.  If
           information is not available (nor calculatable from other
           information sources), return undef.
 Returns : a scalar value or undef if unavailable
 Args    : [optional] scalar value to set the significance
bitscodeprevnextTop
 Usage     : $hit_object->bits();
 Purpose   : Gets the bit score of the best HSP for the current hit.
 Example   : $bits = $hit_object->bits();
 Returns   : Integer or undef if bit score is not set
 Argument  : n/a
 Comments  : For BLAST1, the non-bit score is listed in the summary line.
See Also : score()
next_hspcodeprevnextTop
 Title    : next_hsp
 Usage    : while( $hsp = $obj->next_hsp()) { ... }
 Function : Returns the next available High Scoring Pair
 Example  : 
 Returns  : Bio::Search::HSP::HSPI object or null if finished
 Args     : none
hspscodeprevnextTop
 Usage     : $hit_object->hsps();
 Purpose   : Get a list containing all HSP objects.
           : Get the numbers of HSPs for the current hit.
 Example   : @hsps = $hit_object->hsps();
           : $num  = $hit_object->hsps();  # alternatively, use num_hsps()
 Returns   : Array context : list of Bio::Search::HSP::BlastHSP.pm objects.
           : Scalar context: integer (number of HSPs).
           :                 (Equivalent to num_hsps()).
 Argument  : n/a. Relies on wantarray
 Throws    : Exception if the HSPs have not been collected.
See Also : hsp(), num_hsps()
num_hspscodeprevnextTop
 Usage     : $hit_object->num_hsps();
 Purpose   : Get the number of HSPs for the present Blast hit.
 Example   : $nhsps = $hit_object->num_hsps();
 Returns   : Integer
 Argument  : n/a
 Throws    : Exception if the HSPs have not been collected.
See Also : hsps()
rewindcodeprevnextTop
 Title   : rewind
 Usage   : $hit->rewind;
 Function: Allow one to reset the HSP iterator to the beginning
           Since this is an in-memory implementation
 Returns : none
 Args    : none
ambiguous_alncodeprevnextTop
 Usage     : $ambig_code = $hit_object->ambiguous_aln();
 Purpose   : Sets/Gets ambiguity code data member.
 Example   : (see usage)
 Returns   : String = 'q', 's', 'qs', '-'
           :   'q'  = query sequence contains overlapping sub-sequences 
           :          while sbjct does not.
           :   's'  = sbjct sequence contains overlapping sub-sequences 
           :          while query does not.
           :   'qs' = query and sbjct sequence contains overlapping sub-sequences
           :          relative to each other.
           :   '-'  = query and sbjct sequence do not contains multiple domains 
           :          relative to each other OR both contain the same distribution
           :          of similar domains.
 Argument  : n/a
 Throws    : n/a
 Comment   : Note: "sbjct" is synonymous with "hit"
overlapcodeprevnextTop
See documentation in Bio::Search::Hit::HitI::overlap()
ncodeprevnextTop
 Usage     : $hit_object->n();
 Purpose   : Gets the N number for the current hit.
           : This is the number of HSPs in the set which was ascribed
           : the lowest P-value (listed on the description line).
           : This number is not the same as the total number of HSPs.
           : To get the total number of HSPs, use num_hsps().
 Example   : $n = $hit_object->n();
 Returns   : Integer
 Argument  : n/a
 Throws    : Exception if HSPs have not been set (BLAST2 reports).
 Comments  : Note that the N parameter is not reported in gapped BLAST2.
           : Calling n() on such reports will result in a call to num_hsps().
           : The num_hsps() method will count the actual number of
           : HSPs in the alignment listing, which may exceed N in
           : some cases.
See Also : num_hsps()
pcodeprevnextTop
 Usage     : $hit_object->p( [format] );
 Purpose   : Get the P-value for the best HSP of the given BLAST hit.
           : (Note that P-values are not provided with NCBI Blast2 reports).
 Example   : $p =  $sbjct->p;
           : $p =  $sbjct->p('exp');  # get exponent only.
           : ($num, $exp) =  $sbjct->p('parts');  # split sci notation into parts
 Returns   : Float or scientific notation number (the raw P-value, DEFAULT).
           : Integer if format == 'exp' (the magnitude of the base 10 exponent).
           : 2-element list (float, int) if format == 'parts' and P-value
           :                is in scientific notation (See Comments).
 Argument  : format: string of 'raw' | 'exp' | 'parts'
           :    'raw' returns value given in report. Default. (1.2e-34)
           :    'exp' returns exponent value only (34)
           :    'parts' returns the decimal and exponent as a 
           :            2-element list (1.2, -34) (See Comments).
 Throws    : Warns if no P-value is defined. Uses expect instead.
 Comments  : Using the 'parts' argument is not recommended since it will not
           : work as expected if the P-value is not in scientific notation.
           : That is, floats are not converted into sci notation before
           : splitting into parts.
See Also : expect(), signif(), Bio::Search::SearchUtils::get_exponent()
hspcodeprevnextTop
 Usage     : $hit_object->hsp( [string] );
 Purpose   : Get a single HSPI object for the present HitI object.
 Example   : $hspObj  = $hit_object->hsp;  # same as 'best'
           : $hspObj  = $hit_object->hsp('best');
           : $hspObj  = $hit_object->hsp('worst');
 Returns   : Object reference for a Bio::Search::HSP::BlastHSP.pm object.
 Argument  : String (or no argument).
           :   No argument (default) = highest scoring HSP (same as 'best').
           :   'best' or 'first' = highest scoring HSP.
           :   'worst' or 'last' = lowest scoring HSP.
 Throws    : Exception if the HSPs have not been collected.
           : Exception if an unrecognized argument is used.
See Also : hsps(), num_hsps()
logical_lengthcodeprevnextTop
 Usage     : $hit_object->logical_length( [seq_type] );
           : (mostly intended for internal use).
 Purpose   : Get the logical length of the hit sequence.
           : If the Blast is a TBLASTN or TBLASTX, the returned length 
           : is the length of the would-be amino acid sequence (length/3).
           : For all other BLAST flavors, this function is the same as length().
 Example   : $len    = $hit_object->logical_length();
 Returns   : Integer 
 Argument  : seq_type = 'query' or 'hit' or 'sbjct' (default = 'query')
             ('sbjct' is synonymous with 'hit')
 Throws    : n/a
 Comments  : This is important for functions like frac_aligned_query()
           : which need to operate in amino acid coordinate space when dealing
           : with [T]BLAST[NX] type reports.
See Also : length(), frac_aligned_query(), frac_aligned_hit()
length_alncodeprevnextTop
 Usage     : $hit_object->length_aln( [seq_type] );
 Purpose   : Get the total length of the aligned region for query or sbjct seq.
           : This number will include all HSPs
 Example   : $len    = $hit_object->length_aln(); # default = query
           : $lenAln = $hit_object->length_aln('query');
 Returns   : Integer 
 Argument  : seq_Type = 'query' or 'hit' or 'sbjct' (Default = 'query')
             ('sbjct' is synonymous with 'hit')
 Throws    : Exception if the argument is not recognized.
 Comments  : This method will report the logical length of the alignment,
           : meaning that for TBLAST[NX] reports, the length is reported
           : using amino acid coordinate space (i.e., nucleotides / 3).
           : 
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically..
           : If you don't want the tiled data, iterate through each HSP
           : calling length() on each (use hsps() to get all HSPs).
See Also : length(), frac_aligned_query(), frac_aligned_hit(), gaps(), Bio::Search::SearchUtils::tile_hsps(), Bio::Search::HSP::BlastHSP::length()
gapscodeprevnextTop
 Usage     : $hit_object->gaps( [seq_type] );
 Purpose   : Get the number of gaps in the aligned query, hit, or both sequences.
           : Data is summed across all HSPs.
 Example   : $qgaps = $hit_object->gaps('query');
           : $hgaps = $hit_object->gaps('hit');
           : $tgaps = $hit_object->gaps();    # default = total (query + hit)
 Returns   : scalar context: integer
           : array context without args: two-element list of integers  
           :    (queryGaps, hitGaps)
           : Array context can be forced by providing an argument of 'list' or 'array'.
           :
           : CAUTION: Calling this method within printf or sprintf is arrray context.
           : So this function may not give you what you expect. For example:
           :          printf "Total gaps: %d", $hit->gaps();
           : Actually returns a two-element array, so what gets printed 
           : is the number of gaps in the query, not the total
           :
 Argument  : seq_type: 'query' | 'hit' or 'sbjct' | 'total' | 'list'  (default = 'total')
             ('sbjct' is synonymous with 'hit')
 Throws    : n/a
 Comments  : If you need data for each HSP, use hsps() and then interate
           : through each HSP object.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically..
           : Not relying on wantarray since that will fail in situations 
           : such as printf "%d", $hit->gaps() in which you might expect to 
           : be printing the total gaps, but evaluates to array context.
See Also : length_aln()
matchescodeprevnextTop
See documentation in Bio::Search::Hit::HitI::matches()
startcodeprevnextTop
 Usage     : $sbjct->start( [seq_type] );
 Purpose   : Gets the start coordinate for the query, sbjct, or both sequences
           : in the BlastHit object. If there is more than one HSP, the lowest start
           : value of all HSPs is returned.
 Example   : $qbeg = $sbjct->start('query');
           : $sbeg = $sbjct->start('hit');
           : ($qbeg, $sbeg) = $sbjct->start();
 Returns   : scalar context: integer 
           : array context without args: list of two integers (queryStart, sbjctStart)
           : Array context can be "induced" by providing an argument of 'list' or 'array'.
 Argument  : In scalar context: seq_type = 'query' or 'hit' or 'sbjct' (default = 'query')
             ('sbjct' is synonymous with 'hit')
 Throws    : n/a
 Comments  : This method requires that all HSPs be tiled. If there is more than one
           : HSP and they have not already been tiled, they will be tiled first automatically..
           : Remember that the start and end coordinates of all HSPs are 
           : normalized so that start < end. Strand information can be
           : obtained by calling $hit->strand().
See Also : end(), range(), strand(),
Bio::Search::HSP::BlastHSP::start
endcodeprevnextTop
 Usage     : $sbjct->end( [seq_type] );
 Purpose   : Gets the end coordinate for the query, sbjct, or both sequences
           : in the BlastHit object. If there is more than one HSP, 
             the largest end
           : value of all HSPs is returned.
 Example   : $qend = $sbjct->end('query');
           : $send = $sbjct->end('hit');
           : ($qend, $send) = $sbjct->end();
 Returns   : scalar context: integer
           : array context without args: list of two integers 
           : (queryEnd, sbjctEnd)
           : Array context can be "induced" by providing an argument 
           : of 'list' or 'array'.
 Argument  : In scalar context: seq_type = 'query' or 'sbjct'
           :  (case insensitive). If not supplied, 'query' is used.
 Throws    : n/a
 Comments  : This method requires that all HSPs be tiled. If there is 
           : more than one HSP and they have not already been tiled, 
           : they will be tiled first automatically..
           : Remember that the start and end coordinates of all HSPs are 
           : normalized so that start < end. Strand information can be
           : obtained by calling $hit->strand().
See Also : start(), range(), strand()
rangecodeprevnextTop
 Usage     : $sbjct->range( [seq_type] );
 Purpose   : Gets the (start, end) coordinates for the query or sbjct sequence
           : in the HSP alignment.
 Example   : ($qbeg, $qend) = $sbjct->range('query');
           : ($sbeg, $send) = $sbjct->range('hit');
 Returns   : Two-element array of integers 
 Argument  : seq_type = string, 'query' or 'hit' or 'sbjct'  (default = 'query')
             ('sbjct' is synonymous with 'hit')
 Throws    : n/a
See Also : start(), end()
frac_identicalcodeprevnextTop
 Usage     : $hit_object->frac_identical( [seq_type] );
 Purpose   : Get the overall fraction of identical positions across all HSPs.
           : The number refers to only the aligned regions and does not
           : account for unaligned regions in between the HSPs, if any.
 Example   : $frac_iden = $hit_object->frac_identical('query');
 Returns   : Float (2-decimal precision, e.g., 0.75).
 Argument  : seq_type: 'query' | 'hit' or 'sbjct' | 'total'
           : default = 'query' (but see comments below).
           : ('sbjct' is synonymous with 'hit')
 Throws    : n/a
 Comments  : Different versions of Blast report different values for the total
           : length of the alignment. This is the number reported in the
           : denominators in the stats section:
           : "Identical = 34/120 Positives = 67/120".
           : NCBI BLAST uses the total length of the alignment (with gaps)
           : WU-BLAST uses the length of the query sequence (without gaps).
           :
           : Therefore, when called with an argument of 'total',
           : this method will report different values depending on the
           : version of BLAST used. Total does NOT take into account HSP
           : tiling, so it should not be used.
           :
           : To get the fraction identical among only the aligned residues,
           : ignoring the gaps, call this method without an argument or 
           : with an argument of 'query' or 'hit'.
           :
           : If you need data for each HSP, use hsps() and then iterate
           : through the HSP objects.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically.
See Also : frac_conserved(), frac_aligned_query(), matches(), Bio::Search::SearchUtils::tile_hsps()
frac_conservedcodeprevnextTop
 Usage     : $hit_object->frac_conserved( [seq_type] );
 Purpose   : Get the overall fraction of conserved positions across all HSPs.
           : The number refers to only the aligned regions and does not
           : account for unaligned regions in between the HSPs, if any.
 Example   : $frac_cons = $hit_object->frac_conserved('hit');
 Returns   : Float (2-decimal precision, e.g., 0.75).
 Argument  : seq_type: 'query' | 'hit' or 'sbjct' | 'total'
           : default = 'query' (but see comments below).
           : ('sbjct' is synonymous with 'hit')
 Throws    : n/a
 Comments  : Different versions of Blast report different values for the total
           : length of the alignment. This is the number reported in the
           : denominators in the stats section:
           : "Positives = 34/120 Positives = 67/120".
           : NCBI BLAST uses the total length of the alignment (with gaps)
           : WU-BLAST uses the length of the query sequence (without gaps).
           :
           : Therefore, when called with an argument of 'total',
           : this method will report different values depending on the
           : version of BLAST used. Total does NOT take into account HSP
           : tiling, so it should not be used.
           :
           : To get the fraction conserved among only the aligned residues,
           : ignoring the gaps, call this method without an argument or 
           : with an argument of 'query' or 'hit'.
           :
           : If you need data for each HSP, use hsps() and then interate
           : through the HSP objects.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically.
See Also : frac_identical(), matches(), Bio::Search::SearchUtils::tile_hsps()
frac_aligned_querycodeprevnextTop
 Usage     : $hit_object->frac_aligned_query();
 Purpose   : Get the fraction of the query sequence which has been aligned
           : across all HSPs (not including intervals between non-overlapping
           : HSPs).
 Example   : $frac_alnq = $hit_object->frac_aligned_query();
 Returns   : Float (2-decimal precision, e.g., 0.75).
 Argument  : n/a
 Throws    : n/a
 Comments  : If you need data for each HSP, use hsps() and then interate
           : through the HSP objects.
           : To compute the fraction aligned, the logical length of the query
           : sequence is used, meaning that for [T]BLASTX reports, the 
           : full length of the query sequence is converted into amino acids
           : by dividing by 3. This is necessary because of the way 
           : the lengths of aligned sequences are computed.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically.
See Also : frac_aligned_hit(), logical_length(), length_aln(), Bio::Search::SearchUtils::tile_hsps()
frac_aligned_hitcodeprevnextTop
 Usage     : $hit_object->frac_aligned_hit();
 Purpose   : Get the fraction of the hit (sbjct) sequence which has been aligned
           : across all HSPs (not including intervals between non-overlapping
           : HSPs).
 Example   : $frac_alnq = $hit_object->frac_aligned_hit();
 Returns   : Float (2-decimal precision, e.g., 0.75).
 Argument  : n/a
 Throws    : n/a
 Comments  : If you need data for each HSP, use hsps() and then interate
           : through the HSP objects.
           : To compute the fraction aligned, the logical length of the sbjct
           : sequence is used, meaning that for TBLAST[NX] reports, the 
           : full length of the sbjct sequence is converted into amino acids
           : by dividing by 3. This is necessary because of the way 
           : the lengths of aligned sequences are computed.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically.
See Also : frac_aligned_query(), matches(), , logical_length(), length_aln(), Bio::Search::SearchUtils::tile_hsps()
num_unaligned_hitcodeprevnextTop
 Usage     : $hit_object->num_unaligned_hit();
 Purpose   : Get the number of the unaligned residues in the hit sequence.
           : Sums across all all HSPs.
 Example   : $num_unaln = $hit_object->num_unaligned_hit();
 Returns   : Integer
 Argument  : n/a
 Throws    : n/a
 Comments  : See notes regarding logical lengths in the comments for frac_aligned_hit().
           : They apply here as well.
           : If you need data for each HSP, use hsps() and then interate
           : through the HSP objects.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically..
See Also : num_unaligned_query(), Bio::Search::SearchUtils::tile_hsps(), frac_aligned_hit()
num_unaligned_querycodeprevnextTop
 Usage     : $hit_object->num_unaligned_query();
 Purpose   : Get the number of the unaligned residues in the query sequence.
           : Sums across all all HSPs.
 Example   : $num_unaln = $hit_object->num_unaligned_query();
 Returns   : Integer
 Argument  : n/a
 Throws    : n/a
 Comments  : See notes regarding logical lengths in the comments for frac_aligned_query().
           : They apply here as well.
           : If you need data for each HSP, use hsps() and then interate
           : through the HSP objects.
           : This method requires that all HSPs be tiled. If they have not
           : already been tiled, they will be tiled first automatically..
See Also : num_unaligned_hit(), frac_aligned_query(), Bio::Search::SearchUtils::tile_hsps()
seq_indscodeprevnextTop
 Usage     : $hit->seq_inds( seq_type, class, collapse );
 Purpose   : Get a list of residue positions (indices) across all HSPs
           : for identical or conserved residues in the query or sbjct sequence.
 Example   : @s_ind = $hit->seq_inds('query', 'identical');
           : @h_ind = $hit->seq_inds('hit', 'conserved');
           : @h_ind = $hit->seq_inds('hit', 'conserved', 1);
 Returns   : Array of integers 
           : May include ranges if collapse is non-zero.
 Argument  : [0] seq_type  = 'query' or 'hit' or 'sbjct'  (default = 'query')
           :                 ('sbjct' is synonymous with 'hit')
           : [1] class     = 'identical' or 'conserved' (default = 'identical')
           :              (can be shortened to 'id' or 'cons')
           :              (actually, anything not 'id' will evaluate to 'conserved').
           : [2] collapse  = boolean, if non-zero, consecutive positions are merged
           :             using a range notation, e.g., "1 2 3 4 5 7 9 10 11" 
           :             collapses to "1-5 7 9-11". This is useful for 
           :             consolidating long lists. Default = no collapse.
 Throws    : n/a.
See Also : Bio::Search::HSP::BlastHSP::seq_inds()
strandcodeprevnextTop
See documentation in Bio::Search::Hit::HitI::strand()
framecodeprevnextTop
See documentation in Bio::Search::Hit::HitI::frame()
rankcodeprevnextTop
 Title   : rank
 Usage   : $obj->rank($newval)
 Function: Get/Set the rank of this Hit in the Query search list
           i.e. this is the Nth hit for a specific query
 Returns : value of rank
 Args    : newvalue (optional)
locuscodeprevnextTop
 Title   : locus
 Usage   : $locus = $hit->locus();
 Function: Retrieve the locus (if available) for the hit
 Returns : a scalar string (empty string if not set)
 Args    : none
each_accession_numbercodeprevnextTop
 Title   : each_accession_number
 Usage   : @each_accession_number = $hit->each_accession_number();
 Function: Get each accession number listed in the description of the hit.
           If there are no alternatives, then only the primary accession will 
           be given
 Returns : list of all accession numbers in the description
 Args    : none
tiled_hspscodeprevnextTop
See documentation in Bio::Search::SearchUtils::tile_hsps()
query_lengthcodeprevnextTop
 Title   : query_length
 Usage   : $obj->query_length($newval)
 Function: Get/Set the query_length
 Returns : value of query_length (a scalar)
 Args    : on set, new value (a scalar or undef, optional)
Methods code
newdescriptionprevnextTop
sub new {
  my($class,@args) = @_;

  my $self = $class->SUPER::new(@args);
  my ($hsps, $name,$query_len,$desc, $acc, $locus, $length,
      $score,$algo,$signif,$bits,
      $rank) = $self->_rearrange([qw(HSPS 
                                     NAME 
                                     QUERY_LEN
                                     DESCRIPTION
                                     ACCESSION
                                     LOCUS
                                     LENGTH SCORE ALGORITHM 
                                     SIGNIFICANCE BITS
                                     RANK )], @args);
  
  defined $query_len && $self->query_length($query_len);

  if( ! defined $name ) { 
      $self->throw("Must have defined a valid name for Hit");
  } else { 
      $self->name($name);
  }  

  defined $acc    && $self->accession($acc);
  defined $locus  && $self->locus($locus);
  defined $desc   && $self->description($desc);
  defined $length && $self->length($length);
  defined $algo   && $self->algorithm($algo);
  defined $signif && $self->significance($signif);
  defined $score  && $self->raw_score($score);
  defined $bits   && $self->bits($bits);
  defined $rank   && $self->rank($rank);

  $self->{'_iterator'} = 0;
  $self->{'_hsps'} = [];
  if( defined $hsps  ) {
      if( ref($hsps) !~ /array/i ) {
          $self->warn("Did not specify a valid array ref for the param HSPS ($hsps)");
      } else {
          while( @$hsps ) { 
              $self->add_hsp(shift @$hsps );
          }
      }
  } 
  return $self;
}
add_hspdescriptionprevnextTop
sub add_hsp {
   my ($self,$hsp) = @_;
   if( !defined $hsp || ! $hsp->isa('Bio::Search::HSP::HSPI') ) { 
       $self->throw("Must provide a valid Bio::Search::HSP::HSPI object to object: $self method: add_hsp value: $hsp");
       return undef;
   }
#   print STDERR "GenericHit::add_hsp()\n";
push @{$self->{'_hsps'}}, $hsp; return scalar @{$self->{'_hsps'}};
}
namedescriptionprevnextTop
sub name {
    my ($self,$value) = @_;
    my $previous = $self->{'_name'};
    if( defined $value || ! defined $previous ) {
        $value = $previous = '' unless defined $value;
        $self->{'_name'} = $value;
    } 
    return $previous;
}
accessiondescriptionprevnextTop
sub accession {
    my ($self,$value) = @_;
    my $previous = $self->{'_accession'};
    if( defined $value || ! defined $previous ) { 
        $value = $previous = '' unless defined $value;
        $self->{'_accession'} = $value;
    } 
        return $previous;
}
descriptiondescriptionprevnextTop
sub description {
    my ($self,$value) = @_;
    my $previous = $self->{'_description'};
    if( defined $value || ! defined $previous ) { 
        $value = $previous = '' unless defined $value;
        $self->{'_description'} = $value;
    } 
    return $previous;
}
lengthdescriptionprevnextTop
sub length {
    my ($self,$value) = @_;
    my $previous = $self->{'_length'};
    if( defined $value || ! defined $previous ) { 
        $value = $previous = 0 unless defined $value;
        $self->{'_length'} = $value;
    } 
    return $previous;
}
algorithmdescriptionprevnextTop
sub algorithm {
    my ($self,$value) = @_;
    my $previous = $self->{'_algorithm'};
    if( defined $value || ! defined $previous ) { 
        $value = $previous = '' unless defined $value;
        $self->{'_algorithm'} = $value;
    } 
    return $previous;
}
raw_scoredescriptionprevnextTop
sub raw_score {
    my ($self,$value) = @_;
    my $previous = $self->{'_score'};
    if( defined $value || ! defined $previous ) { 
        $value = $previous = '' unless defined $value;
        $self->{'_score'} = $value;
    } 
    return $previous;
}
scoredescriptionprevnextTop
sub score {
 shift->raw_score(@_);
}
significancedescriptionprevnextTop
sub significance {
    my ($self,$value) = @_;
    my $previous = $self->{'_significance'};
    if( defined $value ) { 
        $self->{'_significance'} = $value;
    } elsif ( ! defined $previous ) {
	unless( defined $self->{'_hsps'}->[0] ) {
	    $self->warn("No HSPs for this Hit (".$self->name.")");
	    return undef;
	}
        # Set the significance of the Hit to that of the top HSP.
$previous = $self->{'_significance'} = ($self->hsps)[0]->significance; } return $previous;
}
bitsdescriptionprevnextTop
sub bits {
 #---------
my ($self,$value) = @_; my $previous = $self->{'_bits'}; if( defined $value ) { $self->{'_bits'} = $value; } elsif ( ! defined $previous ) { # Set the significance of the Hit to that of the top HSP.
unless( defined $self->{'_hsps'}->[0] ) { $self->warn("No HSPs for this Hit (".$self->name.")"); return undef; } $previous = $self->{'_bits'} = ($self->hsps)[0]->bits; } return $previous;
}
next_hspdescriptionprevnextTop
sub next_hsp {
    my ($self) = @_;
    $self->{'_iterator'} = 0 unless defined $self->{'_iterator'};
    return undef if $self->{'_iterator'} > scalar @{$self->{'_hsps'}};
    return $self->{'_hsps'}->[$self->{'_iterator'}++];
}
hspsdescriptionprevnextTop
sub hsps {
#---------
my $self = shift; if (not ref $self->{'_hsps'}) { $self->throw("Can't get HSPs: data not collected."); } return wantarray # returning list containing all HSPs.
? @{$self->{'_hsps'}} # returning number of HSPs.
: scalar(@{$self->{'_hsps'}});
}
num_hspsdescriptionprevnextTop
sub num_hsps {
    my $self = shift;
    
    if (not defined $self->{'_hsps'}) {
        $self->throw("Can't get HSPs: data not collected.");
    }

    return scalar(@{$self->{'_hsps'}});
}
rewinddescriptionprevnextTop
sub rewind {
   my ($self) = @_;
   $self->{'_iterator'} = 0;
}
ambiguous_alndescriptionprevnextTop
sub ambiguous_aln {
 #--------------------
my $self = shift; if(@_) { $self->{'_ambiguous_aln'} = shift; } $self->{'_ambiguous_aln'} || '-';
}
overlapdescriptionprevnextTop
sub overlap {
 #-------------
my $self = shift; if(@_) { $self->{'_overlap'} = shift; } defined $self->{'_overlap'} ? $self->{'_overlap'} : 0;
}
ndescriptionprevnextTop
sub n {
 #-----
my $self = shift; # The check for $self->{'_n'} is a remnant from the 'query' mode days
# in which the sbjct object would collect data from the description
# line only.
my ($n); if(not defined($self->{'_n'})) { if( $self->hsp ) { $n = $self->hsp->n; } } else { $n = $self->{'_n'}; } $n ||= $self->num_hsps; return $n;
}
pdescriptionprevnextTop
sub p {
 #--------
# Some duplication of logic for p(), expect() and signif() for the sake of performance.
my ($self, $fmt) = @_; my $val = $self->{'_p'}; # $val can be zero.
if(not defined $val) { # P-value not defined, must be a NCBI Blast2 report.
# Use expect instead.
$self->warn( "P-value not defined. Using expect() instead."); $val = $self->{'_expect'}; } return $val if not $fmt or $fmt =~ /^raw/i; ## Special formats: exponent-only or as list.
return &Bio::Search::SearchUtils::get_exponent($val) if $fmt =~ /^exp/i; return (split (/eE/, $val)) if $fmt =~ /^parts/i; ## Default: return the raw P-value.
return $val;
}
hspdescriptionprevnextTop
sub hsp {
#----------
my( $self, $option ) = @_; $option ||= 'best'; if (not ref $self->{'_hsps'}) { $self->throw("Can't get HSPs: data not collected."); } my @hsps = @{$self->{'_hsps'}}; return $hsps[0] if $option =~ /best|first|1/i; return $hsps[$#hsps] if $option =~ /worst|last/i; $self->throw("Can't get HSP for: $option\n" . "Valid arguments: 'best', 'worst'");
}
logical_lengthdescriptionprevnextTop
sub logical_length {
#--------------------
my $self = shift; my $seqType = shift || 'query'; $seqType = 'sbjct' if $seqType eq 'hit'; my $length; # For the sbjct, return logical sbjct length
if( $seqType eq 'sbjct' ) { $length = $self->length; # Adjust length based on BLAST flavor.
if($self->algorithm =~ /TBLAST[NX]/ ) { $length /= 3;
} } else { # Otherwise, return logical query length
$length = $self->query_length(); $self->throw("Must have defined query_len") unless ( $length ); # Adjust length based on BLAST flavor.
if($self->algorithm =~ /T?BLASTX/ ) { $length /= 3;
} } return int($length);
}
length_alndescriptionprevnextTop
sub length_aln {
#---------------
my( $self, $seqType, $num ) = @_; $seqType ||= 'query'; $seqType = 'sbjct' if $seqType eq 'hit'; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; if( defined $num) { return $self->{'_length_aln_'.$seqType} = $num; } my $data = $self->{'_length_aln_'.$seqType}; ## If we don't have data, figure out what went wrong.
if(!$data) { $self->throw("Can't get length aln for sequence type\" $seqType\". " . "Valid types are 'query', 'hit', 'sbjct' ('sbjct' = 'hit')"); } return $data;
}
gapsdescriptionprevnextTop
sub gaps {
#----------
my( $self, $seqType, $num ) = @_; $seqType ||= (wantarray ? 'list' : 'total'); $seqType = 'sbjct' if $seqType eq 'hit'; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; $seqType = lc($seqType); if( defined $num ) { $self->throw("Can't set gaps for seqType '$seqType'. Must be 'query' or 'hit'\n") unless ($seqType eq 'sbjct' or $seqType eq 'query'); return $self->{'_gaps_'.$seqType} = $num; } elsif($seqType =~ /list|array/i) { return ($self->{'_gaps_query'}, $self->{'_gaps_sbjct'}); } elsif($seqType eq 'total') { return ($self->{'_gaps_query'} + $self->{'_gaps_sbjct'}) || 0; } else { return $self->{'_gaps_'.$seqType} || 0; }
}
matchesdescriptionprevnextTop
sub matches {
#---------------
my( $self, $arg1, $arg2) = @_; my(@data,$data); Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; unless( $arg1 ) { @data = ($self->{'_totalIdentical'}, $self->{'_totalConserved'}); return @data if @data; } else { if( defined $arg2 ) { $self->{'_totalIdentical'} = $arg1; $self->{'_totalConserved'} = $arg2; return ( $arg1, $arg2 ); } elsif($arg1 =~ /^id/i) { $data = $self->{'_totalIdentical'}; } else { $data = $self->{'_totalConserved'}; } return $data if $data; } ## Something went wrong if we make it to here.
$self->throw("Can't get identical or conserved data: no data.");
}
startdescriptionprevnextTop
sub start {
#----------
my ($self, $seqType, $num) = @_; $seqType ||= (wantarray ? 'list' : 'query'); $seqType = 'sbjct' if $seqType eq 'hit'; if( defined $num ) { $seqType = "_\L$seqType\E"; return $self->{$seqType.'Start'} = $num; } # If there is only one HSP, defer this call to the solitary HSP.
if($self->num_hsps == 1) { return $self->hsp->start($seqType); } else { &Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; if($seqType =~ /list|array/i) { return ($self->{'_queryStart'}, $self->{'_sbjctStart'}); } else { ## Sensitive to member name changes.
$seqType = "_\L$seqType\E"; return $self->{$seqType.'Start'}; } }
}
enddescriptionprevnextTop
sub end {
#----------
my ($self, $seqType, $num) = @_; $seqType ||= (wantarray ? 'list' : 'query'); $seqType = 'sbjct' if $seqType eq 'hit'; if( defined $num ) { $seqType = "_\L$seqType\E"; return $self->{$seqType.'Stop'} = $num; } # If there is only one HSP, defer this call to the solitary HSP.
if($self->num_hsps == 1) { return $self->hsp->end($seqType); } else { Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; if($seqType =~ /list|array/i) { return ($self->{'_queryStop'}, $self->{'_sbjctStop'}); } else { ## Sensitive to member name changes.
$seqType = "_\L$seqType\E"; return $self->{$seqType.'Stop'}; } }
}
rangedescriptionprevnextTop
sub range {
#----------
my ($self, $seqType) = @_; $seqType ||= 'query'; $seqType = 'sbjct' if $seqType eq 'hit'; return ($self->start($seqType), $self->end($seqType));
}
frac_identicaldescriptionprevnextTop
sub frac_identical {
#------------------
my ($self, $seqType) = @_; $seqType ||= 'query'; $seqType = 'sbjct' if $seqType eq 'hit'; ## Sensitive to member name format.
$seqType = lc($seqType); Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; my $ident = $self->matches('id'); my $total = $self->length_aln($seqType); my $ratio = $ident / $total;
my $ratio_rounded = sprintf( "%.3f", $ratio); # Round down iff normal rounding yields 1 (just like blast)
$ratio_rounded = 0.999 if (($ratio_rounded == 1) && ($ratio < 1)); return $ratio_rounded;
}
frac_conserveddescriptionprevnextTop
sub frac_conserved {
#--------------------
my ($self, $seqType) = @_; $seqType ||= 'query'; $seqType = 'sbjct' if $seqType eq 'hit'; ## Sensitive to member name format.
$seqType = lc($seqType); Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; my $consv = $self->matches('cons'); my $total = $self->length_aln($seqType); my $ratio = $consv / $total;
my $ratio_rounded = sprintf( "%.3f", $ratio); # Round down iff normal rounding yields 1 (just like blast)
$ratio_rounded = 0.999 if (($ratio_rounded == 1) && ($ratio < 1)); return $ratio_rounded;
}
frac_aligned_querydescriptionprevnextTop
sub frac_aligned_query {
#----------------------
my $self = shift; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; sprintf( "%.2f", $self->length_aln('query') /
$self->logical_length('query'));
}
frac_aligned_hitdescriptionprevnextTop
sub frac_aligned_hit {
#--------------------
my $self = shift; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; sprintf( "%.2f", $self->length_aln('sbjct') / $self->logical_length('sbjct'));
}
num_unaligned_hitdescriptionprevnextTop
sub num_unaligned_hit {
#---------------------
my $self = shift; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; my $num = $self->logical_length('sbjct') - $self->length_aln('sbjct'); ($num < 0 ? 0 : $num );
}
num_unaligned_querydescriptionprevnextTop
sub num_unaligned_query {
#-----------------------
my $self = shift; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; my $num = $self->logical_length('query') - $self->length_aln('query'); ($num < 0 ? 0 : $num );
}
seq_indsdescriptionprevnextTop
sub seq_inds {
#-------------
my ($self, $seqType, $class, $collapse) = @_; $seqType ||= 'query'; $class ||= 'identical'; $collapse ||= 0; $seqType = 'sbjct' if $seqType eq 'hit'; my (@inds, $hsp); foreach $hsp ($self->hsps) { # This will merge data for all HSPs together.
push @inds, $hsp->seq_inds($seqType, $class); } # Need to remove duplicates and sort the merged positions.
if(@inds) { my %tmp = map { $_, 1 } @inds; @inds = sort {$a <=> $b} keys %tmp; } $collapse ? &Bio::Search::SearchUtils::collapse_nums(@inds) : @inds;
}
stranddescriptionprevnextTop
sub strand {
#----------
my ($self, $seqType, $strnd) = @_; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; $seqType ||= (wantarray ? 'list' : 'query'); $seqType = 'sbjct' if $seqType eq 'hit'; $seqType = lc($seqType); if( defined $strnd ) { $self->throw("Can't set strand for seqType '$seqType'. Must be 'query' or 'hit'\n") unless ($seqType eq 'sbjct' or $seqType eq 'query'); return $self->{'_strand_'.$seqType} = $strnd; } my ($qstr, $hstr); # If there is only one HSP, defer this call to the solitary HSP.
if($self->num_hsps == 1) { return $self->hsp->strand($seqType); } elsif( defined $self->{'_strand_query'}) { # Get the data computed during hsp tiling.
$qstr = $self->{'_strand_query'}; $hstr = $self->{'_strand_sbjct'} } else { # otherwise, iterate through all HSPs collecting strand info.
# This will return the string "-1/1" if there are HSPs on different strands.
# NOTE: This was the pre-10/21/02 procedure which will no longer be used,
# (unless the above elsif{} is commented out).
my (%qstr, %hstr); foreach my $hsp( $self->hsps ) { my ( $q, $h ) = $hsp->strand(); $qstr{ $q }++; $hstr{ $h }++; } $qstr = join( '/', sort keys %qstr); $hstr = join( '/', sort keys %hstr); } if($seqType =~ /list|array/i) { return ($qstr, $hstr); } elsif( $seqType eq 'query' ) { return $qstr; } else { return $hstr; }
}
framedescriptionprevnextTop
sub frame {
 #----------
my( $self, $frm ) = @_; Bio::Search::SearchUtils::tile_hsps($self) unless $self->tiled_hsps; if( defined $frm ) { return $self->{'_frame'} = $frm; } # The check for $self->{'_frame'} is a remnant from the 'query' mode days
# in which the sbjct object would collect data from the description line only.
my ($frame); if(not defined($self->{'_frame'})) { $frame = $self->hsp->frame; } else { $frame = $self->{'_frame'}; } return $frame;
}
rankdescriptionprevnextTop
sub rank {
    my $self = shift;
    return $self->{'_rank'} = shift if @_;
    return $self->{'_rank'} || 1;
}
locusdescriptionprevnextTop
sub locus {
    my ($self,$value) = @_;
    my $previous = $self->{'_locus'};
    if( defined $value || ! defined $previous ) { 
      unless (defined $value) {
        if ($self->{'_name'} =~/(gb|emb|dbj|ref)\|(.*)\|(.*)/) {
                  $value = $previous = $3;
                } else {
          $value = $previous = '';
        }
      }
          $self->{'_locus'} = $value;
    } 
        return $previous;
}
each_accession_numberdescriptionprevnextTop
sub each_accession_number {
    my ($self,$value) = @_;
    my $desc = $self->{'_description'};
    #put primary accnum on the list
my @accnums; push (@accnums,$self->{'_accession'}); if( defined $desc ) { while ($desc =~ /(\b\S+\|\S*\|\S*\s?)/g) { my $id = $1; my ($acc, $version); if ($id =~ /(gb|emb|dbj|sp|pdb|bbs|ref|tp[gde])\|(.*)\|(.*)/) { ($acc, $version) = split /\./, $2; } elsif ($id =~ /(pir|prf|pat|gnl)\|(.*)\|(.*)/) { ($acc, $version) = split /\./, $3; } elsif( $id =~ /(gim|gi|bbm|bbs|lcl)\|(\d*)/) { $acc = $id; } elsif( $id =~ /(oth)\|(.*)\|(.*)\|(.*)/ ) { # discontinued...
($acc,$version) = ($2); } else { #punt, not matching the db's at ftp://ftp.ncbi.nih.gov/blast/db/README
#Database Name Identifier Syntax
#============================ ========================
#GenBank gb|accession|locus
#EMBL Data Library emb|accession|locus
#DDBJ, DNA Database of Japan dbj|accession|locus
#NBRF PIR pir||entry
#Protein Research Foundation prf||name
#SWISS-PROT sp|accession|entry name
#Brookhaven Protein Data Bank pdb|entry|chain
#Patents pat|country|number
#GenInfo Backbone Id bbs|number
#General database identifier gnl|database|identifier
#NCBI Reference Sequence ref|accession|locus
#Local Sequence identifier lcl|identifier
$acc=$id; } push(@accnums, $acc); } } return @accnums;
}
tiled_hspsdescriptionprevnextTop
sub tiled_hsps {
     my $self = shift;
    return $self->{'_tiled_hsps'} = shift if @_;
    return $self->{'_tiled_hsps'};
}
query_lengthdescriptionprevnextTop
sub query_length {
    my $self = shift;

    return $self->{'_query_length'} = shift if @_;
    return $self->{'_query_length'};
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
  bioperl-l@bioperl.org              - General discussion
  http://bioperl.org/MailList.shtml  - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
email or the web:
  bioperl-bugs@bioperl.org
  http://bugzilla.bioperl.org/
AUTHOR - Jason Stajich and Steve ChervitzTop
Email jason@bioperl.org
Email sac@bioperl.org
CONTRIBUTORSTop
Additional contributors names and emails here
APPENDIXTop
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Bio::Search::Hit::HitI methodsTop
Implementation of Bio::Search::Hit::HitI methods
frac_aligned_sbjctTop
Same as frac_aligned_hit()
num_unaligned_sbjctTop
Same as num_unaligned_hit()