Bio::SearchIO
wise
Summary
Bio::SearchIO::wise - Parsing of wise output as alignments
Package variables
No package variables defined.
Included modules
Inherit
Synopsis
use Bio::SearchIO;
my $parser = new Bio::SearchIO(-file => 'file.genewise',
-format => 'wise',
-wisetype=> 'genewise');
while( my $result = $parser->next_result ) {}
Description
This object parsers Wise output using Bio::Tools::Genewise or
Bio::Tools::Genomewise as a helper.
Methods
Methods description
Title : next_result
Usage : my $hit = $searchio->next_result;
Function: Returns the next Result from a search
Returns : Bio::Search::Result::ResultI object
Args : none |
Title : start_element
Usage : $eventgenerator->start_element
Function: Handles a start element event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name' |
Title : start_element
Usage : $eventgenerator->end_element
Function: Handles an end element event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name' |
Title : element
Usage : $eventhandler->element({'Name' => $name, 'Data' => $str});
Function: Convience method that calls start_element, characters, end_element
Returns : none
Args : Hash ref with the keys 'Name' and 'Data' |
Title : characters
Usage : $eventgenerator->characters($str)
Function: Send a character events
Returns : none
Args : string |
Title : within_element
Usage : if( $eventgenerator->within_element($element) ) {}
Function: Test if we are within a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name |
Title : in_element
Usage : if( $eventgenerator->in_element($element) ) {}
Function: Test if we are in a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name |
Title : start_document
Usage : $eventgenerator->start_document
Function: Handle a start document event
Returns : none
Args : none |
Title : end_document
Usage : $eventgenerator->end_document
Function: Handles an end document event
Returns : Bio::Search::Result::ResultI object
Args : none |
Title : wise
Usage : $obj->wise($newval)
Function: Get/Set the Wise object parser
Returns : value of wise (a scalar)
Args : on set, new value (a scalar or undef, optional) |
Title : wisetype
Usage : $obj->wisetype($newval)
Function: Wise program type
Returns : value of wisetype (a scalar)
Args : on set, new value (a scalar or undef, optional) |
Methods code
sub _initialize
{ my ($self,@args) = @_;
my ( $wisetype, $file,$fh ) =
$self->_rearrange([qw(WISETYPE FILE FH)], @args);
my @newargs;
while( @args ) {
my $a = shift @args;
if( $a =~ /FILE|FH/i ) {
shift @args;
next;
}
push @newargs, $a, shift @args;
}
$self->SUPER::_initialize(@newargs);
$self->{'_handler_cache'} = $self->_eventHandler;
$self->wisetype($wisetype);
my @ioargs;
if( $fh ) {
push @ioargs, ('-fh' => $fh);
} elsif( $file ) {
push @ioargs, ('-file' => $file);
}
if( $wisetype =~ /genewise/i ) {
$self->wise(new Bio::Tools::Genewise(@ioargs));
} elsif( $wisetype =~ /genomewise/i ) {
$self->wise(new Bio::Tools::Genomewise(@ioargs));
} else {
$self->throw("Must supply a -wisetype to ".ref($self)." which is one of 'genomewise' 'genewise'\n");
}
return $self;} |
sub next_result
{ my ($self) = @_;
return undef unless $self->wise;
my $prediction = $self->wise->next_prediction;
return undef unless $prediction;
$self->{'_reporttype'} = uc $self->wisetype;
$self->start_element({'Name' => 'WiseOutput'});
$self->element({'Name' => 'WiseOutput_program',
'Data' => $self->wisetype});
$self->element({'Name' => 'WiseOutput_query-def',
'Data' => $self->wise->_target_id});
my @transcripts = $prediction->transcripts;
foreach my $transcript ( @transcripts ) {
my @exons = $transcript->exons;
my $protid;
$self->start_element({'Name' => 'Hit'});
if( $exons[0]->has_tag('supporting_feature') ) {
my ($supporting_feature) = $exons[0]->get_tag_values('supporting_feature');
$protid = $supporting_feature->feature2->seq_id;
$self->element({'Name' => 'Hit_id',
'Data' => $protid});
}
$self->element({'Name' => 'Hit_score',
'Data' => $exons[0]->score});
foreach my $exon ( @exons ) {
$self->start_element({'Name' => 'Hsp'});
if( $exon->strand < 0 ) {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->end});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->start});
} else {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->start});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->end});
}
$self->element({'Name' => 'Hsp_score',
'Data' => $exon->score});
if( $exon->has_tag('supporting_feature') ) {
my ($sf) = $exon->get_tag_values('supporting_feature');
my $protein = $sf->feature2;
if( $protein->strand < 0 ) {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->end});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->start});
} else {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->start});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->end});
}
}
$self->element({'Name' => 'Hsp_identity',
'Data' => 0});
$self->element({'Name' => 'Hsp_positive',
'Data' => 0});
$self->end_element({'Name' => 'Hsp'});
}
$self->end_element({'Name' => 'Hit'});
}
$self->end_element({'Name' => 'WiseOutput'});
return $self->end_document();} |
sub start_element
{ my ($self,$data) = @_;
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
if( $type ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("start_%s",lc $type);
$self->_eventHandler->$func($data->{'Attributes'});
}
unshift @{$self->{'_elements'}}, $type;
if($type eq 'result') {
$self->{'_values'} = {};
$self->{'_result'}= undef;
}
} } |
sub end_element
{ my ($self,$data) = @_;
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
my $rc;
if( $type = $MODEMAP{$nm} ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("end_%s",lc $type);
$rc = $self->_eventHandler->$func($self->{'_reporttype'},
$self->{'_values'});
}
shift @{$self->{'_elements'}};
} elsif( $MAPPING{$nm} ) {
if ( ref($MAPPING{$nm}) =~ /hash/i ) {
my $key = (keys %{$MAPPING{$nm}})[0];
$self->{'_values'}->{$key}->{$MAPPING{$nm}->{$key}} = $self->{'_last_data'};
} else {
$self->{'_values'}->{$MAPPING{$nm}} = $self->{'_last_data'};
}
} else {
$self->debug( "unknown nm $nm, ignoring\n");
}
$self->{'_last_data'} = ''; $self->{'_result'} = $rc if( defined $type && $type eq 'result' );
return $rc;} |
sub element
{ my ($self,$data) = @_;
$self->start_element($data);
$self->characters($data);
$self->end_element($data); } |
sub characters
{ my ($self,$data) = @_;
return unless ( defined $data->{'Data'} && $data->{'Data'} !~ /^\s+$/ );
$self->{'_last_data'} = $data->{'Data'};} |
sub within_element
{ my ($self,$name) = @_;
return 0 if ( ! defined $name &&
! defined $self->{'_elements'} ||
scalar @{$self->{'_elements'}} == 0) ;
foreach ( @{$self->{'_elements'}} ) {
if( $_ eq $name ) {
return 1;
}
}
return 0;} |
sub in_element
{ my ($self,$name) = @_;
return 0 if ! defined $self->{'_elements'}->[0];
return ( $self->{'_elements'}->[0] eq $name)} |
sub start_document
{ my ($self) = @_;
$self->{'_lasttype'} = '';
$self->{'_values'} = {};
$self->{'_result'}= undef;
$self->{'_elements'} = [];
$self->{'_reporttype'} = 'exonerate';} |
sub end_document
{ my ($self,@args) = @_;
return $self->{'_result'};} |
sub write_result
{ my ($self, $blast, @args) = @_;
if( not defined($self->writer) ) {
$self->warn("Writer not defined. Using a $DEFAULT_WRITER_CLASS");
$self->writer( $DEFAULT_WRITER_CLASS->new() );
}
$self->SUPER::write_result( $blast, @args );} |
sub result_count
{ my $self = shift;
return $self->{'_result_count'};} |
sub report_count
{ shift->result_count } |
sub wise
{ my $self = shift;
return $self->{'wise'} = shift if @_;
return $self->{'wise'};} |
sub wisetype
{ my $self = shift;
return $self->{'wisetype'} = shift if @_;
return $self->{'wisetype'};} |
General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing lists
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
http://bugzilla.bioperl.org/
| AUTHOR - Jason Stajich | Top |
Email jason-at-bioperl-dot-org
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Title : new
Usage : my $obj = new Bio::SearchIO::wise();
Function: Builds a new Bio::SearchIO::wise object
Returns : an instance of Bio::SearchIO::wise
Args : -wise => a Bio::Tools::Genewise or Bio::Tools::Genomewise object