Bio::Seq PrimaryQual
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Summary
Bio::Seq::PrimaryQual - Bioperl lightweight Quality Object
Package variables
No package variables defined.
Included modules
Bio::Root::Root
Bio::Seq::QualI
Inherit
Bio::Root::Root Bio::Seq::QualI
Synopsis
 use Bio::Seq::PrimaryQual;

 # you can use either a space-delimited string for quality

 my $string_quals = "10 20 30 40 50 40 30 20 10";
 my $qualobj = Bio::Seq::PrimaryQual->new
 	      ( '-qual' => $string_quals,
 		'-id'  => 'QualityFragment-12',
 		'-accession_number' => 'X78121',
 		);

 # _or_ you can use an array of quality values

 my @q2 = split/ /,$string_quals;
 $qualobj = Bio::Seq::PrimaryQual->new( '-qual' => \@q2,
       '-primary_id'     =>      'chads primary_id',
       '-desc'           =>      'chads desc',
       '-accession_number' => 'chads accession_number',
      '-id'             =>      'chads id'
      );

 # to get the quality values out:

 my @quals = @{$qualobj->qual()};

 # to give _new_ quality values

 my $newqualstring = "50 90 1000 20 12 0 0";
 $qualobj->qual($newqualstring);
Description
This module provides a mechanism for storing quality
values. Much more useful as part of
Bio::Seq::SeqWithQuality where these quality values
are associated with the sequence information.
Methods
newDescriptionCode
qualDescriptionCode
validate_qualDescriptionCode
subqualDescriptionCode
display_idDescriptionCode
accession_numberDescriptionCode
primary_idDescriptionCode
descDescriptionCode
idDescriptionCode
lengthDescriptionCode
qualatDescriptionCode
to_stringDescriptionCode
to_string_automatic
No description
Code
Methods description
new()code    nextTop
 Title   : new()
 Usage   : $qual = Bio::Seq::PrimaryQual->new
	( -qual => '10 20 30 40 50 50 20 10',
	  -id  => 'human_id',
	  -accession_number => 'AL000012',
	);

 Function: Returns a new Bio::Seq::PrimaryQual object from basic 
	constructors, being a string _or_ a reference to an array for the
	sequence and strings for id and accession_number. Note that you
	can provide an empty quality string.
 Returns : a new Bio::Seq::PrimaryQual object
qual()codeprevnextTop
 Title   : qual()
 Usage   : @quality_values  = @{$obj->qual()};
 Function: Returns the quality as a reference to an array containing the
           quality values. The individual elements of the quality array are
           not validated and can be any numeric value.
 Returns : A reference to an array.
validate_qual($qualstring)codeprevnextTop
 Title	 : validate_qual($qualstring)
 Usage	 : print("Valid.") if { &validate_qual($self,$qualities); }
 Function: Make sure that the quality, if it has length > 0, contains at
	least one digit. Note that quality strings are parsed into arrays
	using split/\d+/,$quality_string, so make sure that your quality
	scalar looks like this if you want it to be parsed properly.
 Returns : 1 for a valid sequence (WHY? Shouldn\'t it return 0? )
 Args    : a scalar (any scalar, why PrimarySeq author?) and a scalar
	containing the string to validate.
subqual($start,$end)codeprevnextTop
 Title   : subqual($start,$end)
 Usage   : @subset_of_quality_values = @{$obj->subqual(10,40)};
 Function: returns the quality values from $start to $end, where the
        first value is 1 and the number is inclusive, ie 1-2 are the
	first two bases of the sequence. Start cannot be larger than
	end but can be equal.
 Returns : A reference to an array.
 Args    : a start position and an end position
display_id()codeprevnextTop
 Title   : display_id()
 Usage   : $id_string = $obj->display_id();
 Function: returns the display id, aka the common name of the Quality
        object.
        The semantics of this is that it is the most likely string to be
        used as an identifier of the quality sequence, and likely to have
        "human" readability.  The id is equivalent to the ID field of the
        GenBank/EMBL databanks and the id field of the Swissprot/sptrembl
        database. In fasta format, the >(\S+) is presumed to be the id,
        though some people overload the id to embed other information.
        Bioperl does not use any embedded information in the ID field,
        and people are encouraged to use other mechanisms (accession
	field for example, or extending the sequence object) to solve
	this. Notice that $seq->id() maps to this function, mainly for
        legacy/convience issues
 Returns : A string
 Args    : None
accession_number()codeprevnextTop
 Title   : accession_number()
 Usage   : $unique_biological_key = $obj->accession_number();
 Function: Returns the unique biological id for a sequence, commonly
        called the accession_number. For sequences from established
        databases, the implementors should try to use the correct
        accession number. Notice that primary_id() provides the unique id
        for the implemetation, allowing multiple objects to have the same
        accession number in a particular implementation. For sequences
        with no accession number, this method should return "unknown".
 Returns : A string
 Args    : None
primary_id()codeprevnextTop
 Title   : primary_id()
 Usage   : $unique_implementation_key = $obj->primary_id();
 Function: Returns the unique id for this object in this implementation.
        This allows implementations to manage their own object ids in a
        way the implementaiton can control clients can expect one id to
        map to one object. For sequences with no accession number, this
        method should return a stringified memory location.
 Returns : A string
 Args    : None
desc()codeprevnextTop
 Title   : desc()
 Usage   : $qual->desc($newval);
           $description = $qual->desc();
 Function: Get/set description text for a qual object
 Example :
 Returns : Value of desc
 Args    : newvalue (optional)
id()codeprevnextTop
 Title   : id()
 Usage   : $id = $qual->id();
 Function: Return the ID of the quality. This should normally be (and
	actually is in the implementation provided here) just a synonym
	for display_id().
 Returns : A string.
 Args    : None.
length()codeprevnextTop
 Title	 : length()
 Usage	 : $length = $qual->length();
 Function: Return the length of the array holding the quality values.
	Under most circumstances, this should match the number of quality
	values but no validation is done when the PrimaryQual object is
	constructed and non-digits could be put into this array. Is this
	a bug? Just enough rope...
 Returns : A scalar (the number of elements in the quality array).
 Args    : None.
qualat($position)codeprevnextTop
 Title   : qualat($position)
 Usage   : $quality = $obj->qualat(10);
 Function: Return the quality value at the given location, where the
        first value is 1 and the number is inclusive, ie 1-2 are the first
        two bases of the sequence. Start cannot be larger than end but can
        be equal.
 Returns : A scalar.
 Args    : A position.
to_string()codeprevnextTop
 Title   : to_string()
 Usage   : $quality = $obj->to_string();
 Function: Return a textual representation of what the object contains.
	For this module, this function will return:
		qual
		display_id
		accession_number
		primary_id
		desc
		id
		length
 Returns : A scalar.
 Args    : None.
Methods code
newdescriptionprevnextTop
sub new {
    my ($class, @args) = @_;
    my $self = $class->SUPER::new(@args);

    # default: turn ON the warnings (duh)
my($qual,$id,$acc,$pid,$desc,$given_id) = $self->_rearrange([qw(QUAL DISPLAY_ID ACCESSION_NUMBER PRIMARY_ID DESC ID )], @args); if( defined $id && defined $given_id ) { if( $id ne $given_id ) { $self->throw("Provided both id and display_id constructor functions. [$id] [$given_id]"); } } if( defined $given_id ) { $id = $given_id; } # note: the sequence string may be empty
$self->qual($qual ? $qual : []); $id && $self->display_id($id); $acc && $self->accession_number($acc); $pid && $self->primary_id($pid); $desc && $self->desc($desc); return $self;
}
qualdescriptionprevnextTop
sub qual {
    my ($self,$value) = @_;
    
    if( ! defined $value || length($value) == 0 ) { 
	$self->{'qual'} ||= [];
    } elsif( ref($value) =~ /ARRAY/i ) {
	# if the user passed in a reference to an array
$self->{'qual'} = $value; } elsif(! $self->validate_qual($value)){ $self->throw("Attempting to set the quality to [$value] which does not look healthy"); } else { $self->{'qual'} = [split(/\s+/,$value)]; } return $self->{'qual'};
}
validate_qualdescriptionprevnextTop
sub validate_qual {
    # how do I validate quality values?
# \d+\s+\d+..., I suppose
my ($self,$qualstr) = @_; # why the CORE?? -- (Because Bio::PrimarySeqI namespace has a
# length method, you have to qualify
# which length to use)
return 0 if (!defined $qualstr || CORE::length($qualstr) <= 0); return 1 if( $qualstr =~ /\d/); return 0;
}
subqualdescriptionprevnextTop
sub subqual {
   my ($self,$start,$end) = @_;

   if( $start > $end ){
       $self->throw("in subqual, start [$start] has to be greater than end [$end]");
   }

   if( $start <= 0 || $end > $self->length ) {
       $self->throw("You have to have start positive and length less than the total length of sequence [$start:$end] Total ".$self->length."");
   }

   # remove one from start, and then length is end-start
$start--; $end--; my @sub_qual_array = @{$self->{qual}}[$start..$end]; # return substr $self->seq(), $start, ($end-$start);
return\@ sub_qual_array;
}
display_iddescriptionprevnextTop
sub display_id {
   my ($obj,$value) = @_;
   if( defined $value) {
      $obj->{'display_id'} = $value;
    }
    return $obj->{'display_id'};
}
accession_numberdescriptionprevnextTop
sub accession_number {
    my( $obj, $acc ) = @_;

    if (defined $acc) {
        $obj->{'accession_number'} = $acc;
    } else {
        $acc = $obj->{'accession_number'};
        $acc = 'unknown' unless defined $acc;
    }
    return $acc;
}
primary_iddescriptionprevnextTop
sub primary_id {
   my ($obj,$value) = @_;
   if( defined $value) {
      $obj->{'primary_id'} = $value;
    }
   return $obj->{'primary_id'};
}
descdescriptionprevnextTop
sub desc {
   my ($obj,$value) = @_;
   if( defined $value) {
      $obj->{'desc'} = $value;
    }
    return $obj->{'desc'};
}
iddescriptionprevnextTop
sub id {
   my ($self,$value) = @_;
   if( defined $value ) {
        return $self->display_id($value);
   }
   return $self->display_id();
}
lengthdescriptionprevnextTop
sub length {
    my $self = shift;
    if (ref($self->{qual}) ne "ARRAY") {
	$self->warn("{qual} is not an array here. Why? It appears to be ".ref($self->{qual})."(".$self->{qual}."). Good thing this can _never_ happen.");
    }
    return scalar(@{$self->{qual}});
}
qualatdescriptionprevnextTop
sub qualat {
    my ($self,$val) = @_;
    my @qualat = @{$self->subqual($val,$val)};
    if (scalar(@qualat) == 1) {
	return $qualat[0];
    }
    else {
	$self->throw("AAAH! qualat provided more then one quality.");
    }
}
to_stringdescriptionprevnextTop
sub to_string {
    my ($self,$out,$result) = shift;
    $out = "qual: ".join(',',@{$self->qual()});
    foreach (qw(display_id accession_number primary_id desc id)) {
	$result = $self->$_();
	if (!$result) { $result = "<unset>"; }
	$out .= "$_: $result\n";
    }
    return $out;
}
to_string_automaticdescriptionprevnextTop
sub to_string_automatic {
    my ($self,$sub_result,$out) = shift;
    foreach (sort keys %$self) {
	print("Working on $_\n");
	eval { $self->$_(); };
	if ($@) { $sub_result = ref($_); }
	elsif (!($sub_result = $self->$_())) {
	    $sub_result = "<unset>";
	}
	if (ref($sub_result) eq "ARRAY") {
	    print("This thing ($_) is an array!\n");
	    $sub_result = join(',',@$sub_result);	
	}
	$out .= "$_: ".$sub_result."\n";
    }
    return $out;
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
  bioperl-l@bioperl.org             - General discussion
  http://bio.perl.org/MailList.html - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via email
or the web:
  bioperl-bugs@bio.perl.org
  http://bugzilla.bioperl.org/
AUTHOR - Chad MatsallaTop
Email bioinformatics@dieselwurks.com
APPENDIXTop
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _