Bio::SeqFeature::Tools
FeatureNamer
Summary
Bio::SeqFeature::Tools::FeatureNamer - generates unique persistent names for features
Package variables
No package variables defined.
Inherit
Synopsis
use Bio::SeqIO;
use Bio::SeqFeature::Tools::FeatureNamer;
# first fetch a genbank SeqI object
$seqio =
Bio::SeqIO->new(-file=>'AE003644.gbk',
-format=>'GenBank');
$seq = $seqio->next_seq();
$namer = Bio::SeqFeature::Tools::FeatureNamer->new;
my @features = $seq->get_SeqFeatures;
foreach my $feature (@features) {
$namer->name_feature($feature) unless $feature->display_name;
}
Description
This is a helper class for providing names for SeqFeatures
The
Bio::SeqFeatureI class provides a display_name
method. Typically the display_name is not set when parsing formats
such as genbank - instead properties such as
label,
product or
gene are set in a somewhat inconsistent manner.
In addition, when generating subfeatures (for example, exons that are
subfeatures of a transcript feature), it is often desirable to name
these subfeatures before either exporting to another format or
reporting to the user.
This module is intended to help given uniform display_names to
features and their subfeatures.
Methods
Methods description
Title : new Usage : $unflattener = Bio::SeqFeature::Tools::FeatureNamer->new(); Function: constructor Example : Returns : a new Bio::SeqFeature::Tools::FeatureNamer Args : see below |
Title : name_feature Usage : $namer->name_feature($sf); Function: sets display_name Example : Returns : Args : Bio::SeqFeatureI
This method calls generate_feature_name() and uses the returned value to set the display_name of the feature |
Title : name_contained_features Usage : $namer->name_contained_features($sf); Function: sets display_name for all features contained by sf Example : Returns : Args : Bio::SeqFeatureI
iterates through all subfeatures of a certain feature (using get_all_SeqFeatures) and names each subfeatures, based on the generated name for the holder feature A subfeature is named by concatenating the generated name of the container feature with the type and a number. For example, if the containing feature is a gene with display name dpp, subfeatures will be named dpp-mRNA-1 dpp-mRNA2 dpp-exon1 dpp-exon2 etc |
Title : generate_feature_name Usage : $name = $namer->generate_feature_name($sf); Function: derives a sensible human readable name for a $sf Example : Returns : str Args : Bio::SeqFeatureI
returns a generated name (but does not actually set display_name). If display_name is already set, the method will return this Otherwise, the name will depend on the property:
label product gene locus_tag (in order of priority) |
Methods code
sub new
{ my($class,@args) = @_;
my $self = $class->SUPER::new(@args);
return $self;
} |
sub name_feature
{ my ($self, $sf) = @_;
my $name = $self->generate_feature_name($sf);
$sf->display_name($name);} |
sub name_contained_features
{ my ($self,$sf) = @_;
my $cname = $self->generate_feature_name($sf);
my @subsfs = $sf->get_all_SeqFeatures;
my %num_by_type = ();
foreach my $ssf (@subsfs) {
my $type = $ssf->primary_tag;
my $num = $num_by_type{$type} || 0;
$num++;
$num_by_type{$type} = $num;
$ssf->display_name("$cname-$type-$num");
}
return;} |
sub generate_feature_name
{ my ($self, $sf) = @_;
my $name = $sf->display_name;
if (!$name) {
if ($sf->has_tag("label")) {
($name) = $sf->get_tag_values("label");
}
elsif ($sf->has_tag("product")) {
($name) = $sf->get_tag_values("product");
}
elsif ($sf->primary_tag eq 'gene' &&
$sf->has_tag("gene")) {
($name) = $sf->get_tag_values("gene");
}
elsif ($sf->primary_tag eq 'gene' &&
$sf->has_tag("locus_tag")) {
($name) = $sf->get_tag_values("locus_tag");
}
else {
$name = $sf->display_name;
}
}
return $name;} |
General documentation
Currently the naming policy is hardcoded. It may be desirable to allow
plugging in variations on naming policies; this could be done either
by subclassing, anonymous subroutines (closures) or
parameterization. Contact the author if you feel you have need for a
different naming policy
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
| AUTHOR - Chris Mungall | Top |
Email: cjm AT fruitfly DOT org
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _