Summary

Bio::Tools::Analysis::Protein::Mitoprot - a wrapper around Mitoprot
server

Package variables

Included modules

Inherit

Bio::Tools::Analysis::SimpleAnalysisBase

Synopsis

  use Bio::Tools::Analysis::Protein::Mitoprot;

  use Bio::PrimarySeq;
  my $seq = new Bio::PrimarySeq
    (-seq=>'IKLCVHHJHJHJHJHJHJHNLAILAKAHLIELALAL',
     -primary_id=>'test'); # a Bio::PrimarySeqI object

  my $mitoprot = Bio::Tools::Analysis::Protein::Mitoprot->new
     ( -seq => $seq
     ); # sequence must be  >!5aa long and start with an M.

  # run Mitoprot prediction on a DNA sequence
  my $mitoprot->run();

  die "Could not get a result" unless $mitoprot->status =~ /^COMPLETED/;

  print $mitoprot->result;     # print raw prediction to STDOUT

  foreach my $feat ( $mitoprot->result('Bio::SeqFeatureI') ) {

      # do something to SeqFeature
      # e.g. print as GFF
      print $feat->gff_string, "\n";
      # or store within the sequence - if it is a Bio::RichSeqI
      $seq->add_SeqFeature($feat);

 }

Description

This class is a wrapper around the Mitoprot web server which
calculates the probability of a sequence containing a mitochondrial
targetting peptide. See http://mips.gsf.de/cgi-bin/proj/medgen/mitofilter for more details.
The results can be obtained in 3 formats:
   The raw text of the program output

  my $rawdata = $analysis_object->result;

   An reference to a hash of scores :

  my $data_ref = $analysis_object->result('parsed'); print "predicted
  export prob is $data_ref->{'export_prob'}\n"; #

   key values of returned hash are input_length, basic_aas, acidic_aas,
export_prob, charge, cleavage_site.
   A Bio::SeqFeature::Generic object

  my $ft = $analysis_object->result(Bio::SeqFeatureI);
  print "export prob is ", ($ft->each_tag_value('export_prob'))[0]  ,"\n";

   This the second implentation of Bio::SimpleAnalysisI which hopefully
will make it easier to write wrappers on various services. This class
uses a web resource and therefore inherits from Bio::WebAgent.

Methods

Methods description

 Usage   : $job->result (...)
 Returns : a result created by running an analysis
 Args    : various

Methods code

sub result {

    my ($self,$value) = @_;
    #make sec feat of above threshold scores #

    my @sig_pdctns;
    my @fts;

    if ($value ) {
        my $result = IO::String->new($self->{'_result'});
        my %results;
        while (my $line = <$result>) {
            #make array of all scores or threshold depending on $value
            next unless $line =~ /\d/ || $line =~ /^Cle/;
            if ($line =~ /^Net[^+\-\d]+  # Net, then anything except +,- or digit
                          ((\+|-)?\d+)/x) #then get charge with optional + or -
                              {
                               $results{'charge'} = $1;
                              } elsif ($line =~ /^Input[^\d]+(\d+)/ ) {
                                  $results{'input_length'} = $1;
                              } elsif ($line =~ /basic[^\d]+(\d+)$/ ) {
                                  $results{'basic_aas'} = $1;
                              } elsif ($line =~ /acidic[^\d]+(\d+)$/) {
                                  $results{'acidic_aas'} = $1;
                              } elsif ($line =~ /^Cleavage[^\d]+(\d+)$/) {
                                  $results{'cleavage_site'} = $1;
                              } elsif ($line =~ /^Cleavage/) {
                                  $results{'cleavage_site'} = 'not predictable';
                              } elsif ($line =~ /^of export[^\d]+((0|1)\.\d+)$/) {
                                  $results{'export_prob'} = $1;
                              }
        }

        if ($value eq 'Bio::SeqFeatureI') {
            push @fts, Bio::SeqFeature::Generic->new
                (
                 -start => 1,
                 -end => ($results{'cleavage_site'} =~
                          /^\d+$/)?$results{'cleavage_site'}:$self->seq->length,
                 -source => 'Mitoprot',
                 -primary => 'Region',
                 -tag =>{
                         export_prob   => $results{'export_prob'},
                         charge        => $results{'charge'},
                         basic_aas     => $results{'basic_aas'},
                         acid_aas      => $results{'acidic_aas'},
                         region_name   => 'Transit_peptide',
                         method        => 'MitoProt',
                         cleavage_site => $results{'cleavage_site'},
                        },
                );
            return @fts;        #return Bioseqfeature array
        }
        ## convert parsed data into a meta array format
        else  {
            return\% results;   # hash based results ref
        }
    }
    return $self->{'_result'};

}

sub _init {

    my $self = shift;
    $self->url($URL);
    $self->{'_ANALYSIS_SPEC'} =$ANALYSIS_SPEC;
    $self->{'_INPUT_SPEC'} =$INPUT_SPEC;
    $self->{'_RESULT_SPEC'} =$RESULT_SPEC;
    $self->{'_ANALYSIS_NAME'} =$ANALYSIS_SPEC->{'name'};
    return $self;

}

sub _process_arguments {

    #extra checking for sequence length
    #mitoprot specific argument testing
    my ($self, $args) = @_;
    #use base checking for existence of mandatory fields
    $self->SUPER::_process_arguments($args) ;

    #then check specifics
    $self->throw ("1st_aa must be M") if $self->seq->subseq(1,1) !~ /M/i;
    $self->throw ("sequence must be at least 15aa long") if $self->seq->length< 15;
    return;

}

sub _run {

    #request submitted by get not by post
    my $self  = shift;
    $self->delay(1);
    $self->sleep;

    $self->status('TERMINATED_BY_ERROR');
    my $url = $self->url . "seq=".lc($self->seq->seq). "&seqnam=";
    my $request = GET $url;
    my $content = $self->request($request);
    my $text = $content->content; #1st reponse

    #remove html stuff
    $text =~ s/.*<PRE>(.*)<\/PRE>.*/$1/s;
    $text =~ s/<[^>]+>//sg;

    $self->status('COMPLETED') if $text ne '' && $self->seq->length > $MIN_LEN;
    $self->{'_result'} = $text;

}

General documentation

Bio::SimpleAnalysisI,
Bio::Tools::Analysis::SimpleAnalysisBase,
Bio::WebAgent

User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:

  http://bugzilla.open-bio.org/

Richard Adams, Richard.Adams@ed.ac.uk,

The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _