Bio::Tools Hmmpfam
SummaryPackage variablesSynopsisDescriptionGeneral documentationMethods
Summary
Bio::Tools::Hmmpfam - Parser for Hmmpfam program
Package variables
No package variables defined.
Included modules
Bio::SeqFeature::FeaturePair
Bio::SeqFeature::Generic
base qw ( Bio::Root::Root Bio::Root::IO )
Synopsis
  use Bio::Tools::Hmmpfam;
my $hmmpfam_parser = new Bio::Tools::Hmmpfam(-fh =>$filehandle );
while( my $hmmpfam_feat = $hmmpfam_parser->next_result ) {
push @hmmpfam_feat, $hmmpfam_feat;
}
Description
Parser for Hmmpfam program. See also Bio::SearchIO::hmmer.
Methods
newDescriptionCode
next_resultDescriptionCode
create_featureDescriptionCode
seqnameDescriptionCode
Methods description
newcode    nextTop
 Title   : new
Usage : my $obj = new Bio::Tools::Hmmpfam(-fh=>$filehandle);
Function: Builds a new Bio::Tools::Hmmpfam object
Returns : Bio::Tools::Hmmpfam
Args : -filename
-fh (filehandle)
next_resultcodeprevnextTop
 Title   : next_result
Usage : my $feat = $hmmpfam_parser->next_result
Function: Get the next result set from parser data
Returns : Bio::SeqFeature::Generic
Args : none
create_featurecodeprevnextTop
 Title   : create_feature
Usage : my $feat=$hmmpfam_parser->create_feature($feature,$seqname)
Function: creates a SeqFeature Generic object
Returns : Bio::SeqFeature::Generic
Args :
seqnamecodeprevnextTop
 Title   :   seqname
Usage : obj->seqname($seqname)
Function: Internal(not to be used directly)
Returns :
Args : seqname
Methods code
newdescriptionprevnextTop
sub new {
      my($class,@args) = @_;

      my $self = $class->SUPER::new(@args);
      $self->_initialize_io(@args);

      return $self;
}
next_resultdescriptionprevnextTop
sub next_result {
    my ($self) = @_;
    my $filehandle;
    
 my $line;

    my $id;
    while ($_=$self->_readline()) {
         $line = $_;
         chomp $line;
    
        if ( $line=~m/^Alignments of top-scoring domains/ ) {
          while( my $rest = $self->_readline() ) { last if $rest =~ m!^//! }
        }

        next if ($line=~m/^Model/ || /^\-/ || /^$/);
        
        if ($line=~m/^Query sequence:\s+(\S+)/) {
           $id = $1;
           $self->seqname($id);
        }
       
       if (my ($hid, $start, $end, $hstart, $hend, $score, $evalue) = $line=~m/^(\S+)\s+\S+\s+(\d+)\s+(\d+)\s+\S+\s+(\d+)\s+(\d+)\s+\S+\s+(\S+)\s+(\S+)/) {
            my %feature;
            
            ($feature{name}) = $self->seqname;
            $feature{raw_score} = $score;
            $feature{p_value} = sprintf ("%.3e", $evalue);
            $feature{score} = $feature{p_value};
            $feature{start} = $start;
            $feature{end} = $end;
            $feature{hname} = $hid;
            $feature{hstart} = $hstart;
            $feature{hend} = $hend;
            ($feature{source}) = 'pfam';
            $feature{primary} = $hid;
            ($feature{program}) = 'pfam';
            ($feature{db}) = 'db1';
            ($feature{logic_name}) = 'hmmpfam';
            my $new_feat = $self->create_feature (\%feature);
            return $new_feat
        
        }
        next;

    }
    return;
}
create_featuredescriptionprevnextTop
sub create_feature {
    my ($self, $feat) = @_;



    my $feature1= Bio::SeqFeature::Generic->new( -seq_id  =>$feat->{name},
                                                -start      =>$feat->{start},
                                                -end        =>$feat->{end},
                                                -score      =>$feat->{score},
                                                -source     =>$feat->{source},
                                                -primary    =>$feat->{primary},
                                                   );
    


    my $feature2= Bio::SeqFeature::Generic->new(
                                                 -start      =>$feat->{hstart},
                                                 -end        =>$feat->{hend},
                                                  );




    my $featurepair = Bio::SeqFeature::FeaturePair->new;
    $featurepair->feature1 ($feature1);
    $featurepair->feature2 ($feature2);
   
   $featurepair->add_tag_value('evalue',$feat->{p_value});
   $featurepair->add_tag_value('percent_id','NULL');
   $featurepair->add_tag_value("hid",$feat->{primary});
    return  $featurepair;
}
seqnamedescriptionprevnextTop
sub seqname {
    my($self,$seqname)=@_;

    if(defined($seqname))
    {
        $self->{'seqname'}=$seqname;
    }

    return $self->{'seqname'};
}
General documentation
FEEDBACKTop
Mailing ListsTop
 User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
  http://bugzilla.open-bio.org/
AUTHOR - Balamurugan KumarasamyTop
 Email: fugui@worf.fugu-sg.org
APPENDIXTop
 The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _