Bio::Tools::Phylo::Molphy
Result
Summary
Bio::Tools::Phylo::Molphy::Result - container for data parsed from a ProtML run
Package variables
No package variables defined.
Inherit
Synopsis
# do not use this object directly, you will get it back as part of a
# Molphy parser
use Bio::Tools::Phylo::Molphy;
my $parser = new Bio::Tools::Phylo::Molphy(-file => 'output.protml');
while( my $r = $parser->next_result ) {
# r is a Bio::Tools::Phylo::Molphy::Result object
# print the model name
print $r->model, "\n";
# get the substitution matrix
# this is a hash of 3letter aa codes -> 3letter aa codes representing
# substitution rate
my $smat = $r->substitution_matrix;
print "Arg -> Gln substitution rate is %d\n",
$smat->{'Arg'}->{'Gln'}, "\n";
# get the transition probablity matrix
# this is a hash of 3letter aa codes -> 3letter aa codes representing
# transition probabilty
my $tmat = $r->transition_probability_matrix;
print "Arg -> Gln transition probablity is %.2f\n",
$tmat->{'Arg'}->{'Gln'}, "\n";
# get the frequency for each of the residues
my $rfreqs = $r->residue_frequencies;
foreach my $residue ( keys %{$rfreqs} ) {
printf "residue %s expected freq: %.2f observed freq: %.2f\n",
$residue,$rfreqs->{$residue}->[0], $rfreqs->{$residue}->[1];
}
my @trees;
while( my $t = $r->next_tree ) {
push @trees, $t;
}
print "search space is ", $r->search_space, "\n",
"1st tree score is ", $trees[0]->score, "\n";
# writing to STDOUT, use -file => '>filename' to specify a file
my $out = new Bio::TreeIO(-format => "newick");
$out->write_tree($trees[0]); # writing only the 1st tree
}
Description
A container for data parsed from a ProtML run.
Methods
Methods description
Title : new Usage : my $obj = new Bio::Tools::Phylo::Molphy::Result(); Function: Builds a new Bio::Tools::Phylo::Molphy::Result object Returns : Bio::Tools::Phylo::Molphy::Result Args : |
Title : model Usage : $obj->model($newval) Function: Returns : value of model Args : newvalue (optional) |
Title : substitution_matrix Usage : my $smat = $result->subsitution_matrix; Function: Get the relative substitution matrix calculated in the ML procedure Returns : reference to hash of hashes where key is the aa/nt name and value is another hash ref which contains keys for all the aa/nt possibilities Args : none |
Title : transition_probability_matrix Usage : my $matrixref = $molphy->transition_probablity_matrix(); Function: Gets the observed transition probability matrix Returns : hash of hashes of aa/nt transition to each other aa/nt Args : Transition matrix type, typically '1PAM-1.0e05' or '1PAM-1.0e07' |
Title : residue_frequencies Usage : my %data = $molphy->residue_frequencies() Function: Get the modeled and expected frequencies for each of the residues in the sequence Returns : hash of either aa (protml) or nt (nucml) frequencies each key will point to an array reference where 1st slot is model's expected frequency 2nd slot is observed frequency in the data $hash{'A'}->[0] = Args : none |
Title : next_tree Usage : my $tree = $factory->next_tree; Function: Get the next tree from the factory Returns : Bio::Tree::TreeI Args : none |
Title : add_tree Usage : $result->add_tree($tree); Function: Adds a tree Returns : integer which is the number of trees stored Args : Bio::Tree::TreeI |
Title : search_space Usage : $obj->search_space($newval) Function: Returns : value of search_space Args : newvalue (optional) |
Methods code
sub new
{ my($class,@args) = @_;
my $self = $class->SUPER::new(@args);
my ($trees,$smat,$freq,
$model, $sspace,
) = $self->_rearrange([qw(TREES SUBSTITUTION_MATRIX
FREQUENCIES
MODEL SEARCH_SPACE)], @args);
if( $trees ) {
if(ref($trees) !~ /ARRAY/i ) {
$self->warn("Must have provided a valid array reference to initialize trees");
} else {
foreach my $t ( @$trees ) {
$self->add_tree($t);
}
}
}
if( ref($smat) =~ /HASH/i ) {
$self->substitution_matrix($smat);
}
if( ref($freq) =~ /HASH/i ) {
$self->residue_frequencies($freq);
}
$model && $self->model($model);
$sspace && $self->search_space($sspace);
$self->{'_treeiterator'} = 0;
return $self;} |
sub model
{ my ($self,$value) = @_;
if( defined $value) {
$self->{'model'} = $value;
}
return $self->{'model'};} |
sub substitution_matrix
{ my ($self,$val) = @_;
if(defined $val ) {
if( ref($val) =~ /HASH/ ) {
foreach my $v (values %{$val} ) {
if( ref($v) !~ /HASH/i ) {
$self->warn("Must be a valid hashref of hashrefs for substition_matrix");
return;
}
}
$self->{'_substitution_matrix'} = $val;
} else {
$self->warn("Must be a valid hashref of hashrefs for substition_matrix");
return;
}
}
return $self->{'_substitution_matrix'};} |
sub transition_probability_matrix
{ my ($self,$type,$val) = @_;
$type = '1PAM-1.0e7' unless defined $type;
if(defined $val ) {
if( ref($val) =~ /HASH/ ) {
foreach my $v (values %{$val} ) {
if( ref($v) !~ /HASH/i ) {
$self->warn("Must be a valid hashref of hashrefs for transition_probability_matrix");
return;
}
}
$self->{'_TPM'}->{$type} = $val;
} else {
$self->warn("Must be a valid hashref of hashrefs for transition_probablity_matrix");
return;
}
}
return $self->{'_TPM'}->{$type};} |
sub residue_frequencies
{ my ($self,$val) = @_;
if(defined $val ) {
if( ref($val) =~ /HASH/ ) {
$self->{'_residue_frequencies'} = $val;
} else {
$self->warn("Must be a valid hashref of hashrefs for residue_frequencies");
}
}
return %{$self->{'_residue_frequencies'}};} |
sub next_tree
{ my ($self,@args) = @_;
return $self->{'_trees'}->[$self->{'_treeiterator'}++] || undef;} |
sub rewind_tree_iterator
{ shift->{'_treeiterator'} = 0;} |
sub add_tree
{ my ($self,$tree) = @_;
if( $tree && ref($tree) && $tree->isa('Bio::Tree::TreeI') ) {
push @{$self->{'_trees'}},$tree;
}
return scalar @{$self->{'_trees'}};} |
sub search_space
{ my ($self,$value) = @_;
if( defined $value) {
$self->{'search_space'} = $value;
}
return $self->{'search_space'};} |
General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
| AUTHOR - Jason Stajich | Top |
Email jason-at-bioperl.org
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Title : rewind_tree_iterator
Usage : $result->rewind_tree()
Function: Rewinds the tree iterator so that next_tree can be
called again from the beginning
Returns : none
Args : none