Bio::DB::Query GenBank
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Bio::DB::Query::GenBank - Build a GenBank Entrez Query
Package variables
No package variables defined.
Included modules
URI::Escape ' uri_unescape '
   use Bio::DB::Query::GenBank;
use Bio::DB::GenBank;
my $query_string = 'Oryza[Organism] AND EST[Keyword]'; my $query = Bio::DB::Query::GenBank->new(-db => 'nucleotide', -query => $query_string, -mindate => '2001', -maxdate => '2002'); print $query->count,"\n"; # get a Genbank database handle my $gb = Bio::DB::GenBank->new(); my $stream = $gb->get_Stream_by_query($query); while (my $seq = $stream->next_seq) { # do something with the sequence object } # initialize the list yourself my $query = Bio::DB::Query::GenBank->new(-ids=>[195052,2981014,11127914]);
This class encapsulates NCBI Entrez queries. It can be used to store
a list of GI numbers, to translate an Entrez query expression into a
list of GI numbers, or to count the number of terms that would be
returned by a query. Once created, the query object can be passed to
a Bio::DB::GenBank object in order to retrieve the entries
corresponding to the query.
Methods description
newcode    nextTop
 Title   : new
Usage : $db = Bio::DB::Query::GenBank->new(@args)
Function: create new query object
Returns : new query object
Args : -db database (see below for allowable values)
-query query string
-mindate minimum date to retrieve from (YYYY/MM/DD)
-maxdate maximum date to retrieve from (YYYY/MM/DD)
-reldate relative date to retrieve from (days)
-datetype date field to use ('edat' or 'mdat')
-ids array ref of gids (overrides query)
-maxids the maximum number of IDs you wish to collect
(defaults to 100)
This method creates a new query object. Typically you will specify a
-db and a -query argument, possibly modified by -mindate, -maxdate, or
-reldate. -mindate and -maxdate specify minimum and maximum dates for
entries you are interested in retrieving, expressed in the form
YYYY/MM/DD. -reldate is used to fetch entries that are more recent
than the indicated number of days.
If you provide an array reference of IDs in -ids, the query will be
ignored and the list of IDs will be used when the query is passed to a
Bio::DB::GenBank object's get_Stream_by_query() method. A variety of
IDs are automatically recognized, including GI numbers, Accession
numbers, Accession.version numbers and locus names.
By default, the query will collect only the first 100 IDs and will
generate an exception if you call the ids() method and the query
returned more than that number. To increase this maximum, set -maxids
to a number larger than the number of IDs you expect to obtain. This
only affects the list of IDs you obtain when you call the ids()
method, and does not affect in any way the number of entries you
receive when you generate a SeqIO stream from the query.
-db option values:
  The most commonly used databases are:
protein nucleotide nuccore nucgss nucest unigene An up to date list of database names supported by NCBI eUtils is always available at:
However, note that not all of these databases return datatypes that are parsable by Bio::DB::GenBank
 Title   : cookie
Usage : ($cookie,$querynum) = $db->cookie
Function: return the NCBI query cookie
Returns : list of (cookie,querynum)
Args : none
NOTE: this information is used by Bio::DB::GenBank in
conjunction with efetch.
 Title   : _request_parameters
Usage : ($method,$base,@params = $db->_request_parameters
Function: return information needed to construct the request
Returns : list of method, url base and key=>value pairs
Args : none
 Title   : count
Usage : $count = $db->count;
Function: return count of number of entries retrieved by query
Returns : integer
Args : none
Returns the number of entries that are matched by the query.
 Title   : _parse_response
Usage : $db->_parse_response($content)
Function: parse out response
Returns : empty
Args : none
Throws : 'unparseable output exception'
 Title   : _generate_id_string
Usage : $string = $db->_generate_id_string
Function: joins IDs together in string (possibly implementation-dependent)
Returns : string of concatenated IDs
Args : array ref of ids (normally passed into the constructor)
Methods code
  @ATTRIBUTES = qw(db reldate mindate maxdate datetype maxids);
  for my $method (@ATTRIBUTES) {
    eval <<END;
sub $method {
my \$self = shift;
my \$d = \$self->{'_$method'};
\$self->{'_$method'} = shift if \@_;
sub new {
  my $class = shift;
  my $self  = $class->SUPER::new(@_);
  my ($query,$db,$reldate,$mindate,$maxdate,$datetype,$ids,$maxids)
  $self->db($db || DEFAULT_DB);
  $reldate  && $self->reldate($reldate);
  $mindate  && $self->mindate($mindate);
  $maxdate  && $self->maxdate($maxdate);
  $maxids   && $self->maxids($maxids);
  $datetype ||= 'mdat';
  $datetype && $self->datetype($datetype);
sub cookie {
  my $self = shift;
  if (@_) {
    $self->{'_cookie'}   = shift;
    $self->{'_querynum'} = shift;

  else {
    @{$self}{qw(_cookie _querynum)};
sub _request_parameters {
  my $self = shift;
  my ($method,$base);
  my @params = map {eval("\$self->$_") ? ($_ => eval("\$self->$_")) : () } @ATTRIBUTES;
  push @params,('usehistory'=>'y','tool'=>'bioperl');
  $method = 'get';
  $base   = ESEARCH;
  push @params,('term'   => $self->query);

  # Providing 'retmax' limits queries to 500 sequences  ?? I don't think so LS
push @params,('retmax' => $self->maxids || MAXENTRY); # And actually, it seems that we need 'retstart' equal to 0 ?? I don't think so LS
# push @params, ('retstart' => 0);
sub count {
  my $self = shift;
  if (@_) {
    my $d = $self->{'_count'};
    $self->{'_count'}   = shift;
    return $d;
  else {
    return $self->{'_count'};
sub _parse_response {
  my $self    = shift;
  my $content = shift;
  if (my ($warning) = $content =~ m!<ErrorList>(.+)</ErrorList>!s) {
$self->warn("Warning(s) from GenBank: $warning\n");
} if (my ($error) = $content =~ /<OutputMessage>([^<]+)/) { $self->throw("Error from Genbank: $error"); } my ($count) = $content =~ /<Count>(\d+)/; my ($max) = $content =~ /<RetMax>(\d+)/; my $truncated = $count > $max; $self->count($count); if (!$truncated) { my @ids = $content =~ /<Id>(\d+)/g; $self->ids(\@ids); } else { $self->debug("ids truncated at $max\n"); } $self->_truncated($truncated); my ($cookie) = $content =~ m!<WebEnv>(\S+)</WebEnv>!;
my ($querykey) = $content =~ m!<QueryKey>(\d+)!;
sub _generate_id_string {
    my ($self, $ids) = @_;
    # this attempts to separate out accs (alphanumeric) from UIDs (numeric only)
# recent changes to esearch has wrought this upon us.. cjf 4/19/07
return sprintf('%s',join('|',map { ($_ =~ m{^\d+$}) ? $_.'[UID]' : $_.'[PACC]' } @$ids)); } 1;
General documentation
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Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
AUTHOR - Lincoln SteinTop
The rest of the documentation details each of the
object methods. Internal methods are usually
preceded with a _
 Title   : ids
Usage : @ids = $db->ids([@ids])
Function: get/set matching ids
Returns : array of sequence ids
Args : (optional) array ref with new set of ids
 Title   : query
Usage : $query = $db->query([$query])
Function: get/set query string
Returns : string
Args : (optional) new query string