Bio::DB SeqFeature
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Bio::DB::SeqFeature -- Normalized feature for use with Bio::DB::SeqFeature::Store
Package variables
No package variables defined.
Included modules
Carp ' croak '
Bio::DB::SeqFeature::NormalizedFeature Bio::DB::SeqFeature::NormalizedTableFeatureI
 use Bio::DB::SeqFeature::Store;
# Open the sequence database my $db = Bio::DB::SeqFeature::Store->new( -adaptor => 'DBI::mysql', -dsn => 'dbi:mysql:test'); my ($feature) = $db->get_features_by_name('ZK909'); my @subfeatures = $feature->get_SeqFeatures(); my @exons_only = $feature->get_SeqFeatures('exon'); # create a new object my $new = $db->new_feature(-primary_tag=>'gene', -seq_id => 'chr3', -start => 10000, -end => 11000); # add a new exon $feature->add_SeqFeature($db->new_feature(-primary_tag=>'exon', -seq_id => 'chr3', -start => 5000, -end => 5551));
The Bio::DB::SeqFeature object is the default SeqFeature class stored
in Bio::DB::SeqFeature databases. It implements both the
Bio::DB::SeqFeature::NormalizedFeatureI and
Bio::DB::SeqFeature::NormalizedTableFeatureI interfaces, which means that its
subfeatures, if any, are stored in the database in a normalized
fashion, and that the parent/child hierarchy of features and
subfeatures are also stored in the database as set of tuples. This
provides efficiencies in both storage and retrieval speed.
Typically you will not create Bio::DB::SeqFeature directly, but will
ask the database to do so on your behalf, as described in
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Methods description
get_SeqFeaturescode    nextTop
 Title   : get_SeqFeature
Usage : @subfeatures = $feature->get_SeqFeatures([@types])
Function: return subfeatures of this feature
Returns : list of subfeatures
Args : list of subfeature primary_tags (optional)
Status : public
This method extends the Bio::SeqFeatureI get_SeqFeatures() slightly by
allowing you to pass a list of primary_tags, in which case only
subfeatures whose primary_tag is contained on the list will be
returned. Without any types passed all subfeatures are returned.
Methods code
sub add_segment {
  my $self = shift;
sub _add_segment {
  my $self       = shift;
  my $normalized = shift;

  my $store      = $self->object_store;
  my $store_parentage = eval{$store->can_store_parentage};

  return         $self->SUPER::_add_segment($normalized,@_)
    unless $normalized && $store_parentage;

  my @segments   = $self->_create_subfeatures($normalized,@_);

  my $pos = "@{$self}{'start','stop','ref','strand'}";

  # fix boundaries
$self->_fix_boundaries(\@segments,1); # freakish fixing of our non-standard Target attribute
$self->_fix_target(\@segments); # write our children out
if ($normalized) { $store->add_SeqFeature($self,@segments); } else { push @{$self->{segments}},@segments; } # write us back to disk
$self->update if $self->primary_id && $pos ne "@{$self}{'start','stop','ref','strand'}"; } # segments can be stored directly in the object (legacy behavior)
# or stored in the database
# an optional list of types can be used to specify which types to return
sub get_SeqFeatures {
  my $self         = shift;
  my @types        = @_;

  my @inline_segs  = exists $self->{segments} ? @{$self->{segments}} : ();
  @inline_segs     = grep {$_->type_match(@types)} @inline_segs if @types;
  my $store        = $self->object_store;

  my @db_segs;

  if ($store && $store->can_store_parentage) {
    if (!@types || $store->subfeature_types_are_indexed) {
      @db_segs = $store->fetch_SeqFeatures($self,@types);
    } else {
      @db_segs     = grep {$_->type_match(@types)} $store->fetch_SeqFeatures($self);

  my @segs         = (@inline_segs,@db_segs);
  foreach (@segs) {
    eval {$_->object_store($store)};
  return @segs;
sub denormalized_segments {
  my $self = shift;
  return exists $self->{segments} ? @{$self->{segments}} : ();
sub denormalized_segment_count {
  my $self = shift;
  return 0 unless exists $self->{segments};
  return scalar @{$self->{segments}};

# for Bio::LocationI compatibility
sub location_type {
 return 'EXACT'
sub feature_id {
General documentation
 Title   : new
Usage : $feature = Bio::DB::SeqFeature::NormalizedFeature->new(@args)
Function: create a new feature
Returns : the new seqfeature
Args : see below
Status : public
This method creates and, if possible stores into a database, a new
Bio::DB::SeqFeature::NormalizedFeature object using the specialized
Bio::DB::SeqFeature class.
The arguments are the same to Bio::SeqFeature::Generic->new() and
Bio::Graphics::Feature->new(). The most important difference is the
-store option, which if present creates the object in a
Bio::DB::SeqFeature::Store database, and the -index option, which
controls whether the feature will be indexed for retrieval (default is
true). Ordinarily, you would only want to turn indexing on when
creating top level features, and off only when storing
subfeatures. The default is on.
Arguments are as follows:
  -seq_id       the reference sequence
-start the start position of the feature
-end the stop position of the feature
-display_name the feature name (returned by seqname)
-primary_tag the feature type (returned by primary_tag)
-source the source tag
-score the feature score (for GFF compatibility)
-desc a description of the feature
-segments a list of subfeatures (see Bio::Graphics::Feature)
-subtype the type to use when creating subfeatures
-strand the strand of the feature (one of -1, 0 or +1)
-phase the phase of the feature (0..2)
-url a URL to link to when rendered with Bio::Graphics
-attributes a hashref of tag value attributes, in which the key is the tag
and the value is an array reference of values
-store a previously-opened Bio::DB::SeqFeature::Store object
-index index this feature if true
  -id           an alias for -display_name
-seqname an alias for -display_name
-display_id an alias for -display_name
-name an alias for -display_name
-stop an alias for end
-type an alias for primary_tag
Bio::SeqFeatureI methodsTop
The following Bio::SeqFeatureI methods are supported:
 seq_id(), start(), end(), strand(), get_SeqFeatures(),
display_name(), primary_tag(), source_tag(), seq(),
location(), primary_id(), overlaps(), contains(), equals(),
intersection(), union(), has_tag(), remove_tag(),
add_tag_value(), get_tag_values(), get_all_tags()
Some methods that do not make sense in the context of a genome
annotation database system, such as attach_seq(), are not supported.
Please see Bio::SeqFeatureI for more details.
 Title   : add_SeqFeature
Usage : $flag = $feature->add_SeqFeature(@features)
Function: Add subfeatures to the feature
Returns : true if successful
Args : list of Bio::SeqFeatureI objects
Status : public
Add one or more subfeatures to the feature. For best results,
subfeatures should be of the same class as the parent feature
(i.e. do not try mixing Bio::DB::SeqFeature::NormalizedFeature with
other feature types).
An alias for this method is add_segment().
 Title   : update
Usage : $flag = $feature->update()
Function: Update feature in the database
Returns : true if successful
Args : none
Status : public
After changing any fields in the feature, call update() to write it to
the database. This is not needed for add_SeqFeature() as update() is
invoked automatically.
 Title   : object_store
Usage : $store = $feature->object_store([$new_store])
Function: get or set the database handle
Returns : current database handle
Args : new database handle (optional)
Status : public
This method will get or set the Bio::DB::SeqFeature::Store object that
is associated with the feature. After changing the store, you should
probably unset the primary_id() of the feature and call update() to ensure
that the object is written into the database as a new feature.
 Title   : overloaded_names
Usage : $overload = $feature->overloaded_names([$new_overload])
Function: get or set overloading of object strings
Returns : current flag
Args : new flag (optional)
Status : public
For convenience, when objects of this class are stringified, they are
represented in the form "primary_tag(display_name)". To turn this
feature off, call overloaded_names() with a false value. You can
invoke this on an individual feature object or on the class:
 Title   : segment
Usage : $segment = $feature->segment
Function: return a Segment object corresponding to feature
Returns : a Bio::DB::SeqFeature::Segment
Args : none
Status : public
This turns the feature into a Bio::DB::SeqFeature::Segment object,
which you can then use to query for overlapping features. See
 @subfeatures = $feature->Exon;
If you use an unknown method that begins with a capital letter, then
the feature autogenerates a call to get_SeqFeatures() using the
lower-cased method name as the primary_tag. In other words
$feature->Exon is equivalent to:
 @subfeature s= $feature->get_SeqFeatures('exon')
 Title   : load_id
Usage : $id = $feature->load_id
Function: get the GFF3 load ID
Returns : the GFF3 load ID (string)
Args : none
Status : public
For features that were originally loaded by the GFF3 loader, this
method returns the GFF3 load ID. This method may not be supported in
future versions of the module.
 Title   : primary_id
Usage : $id = $feature->primary_id([$new_id])
Function: get/set the database ID of the feature
Returns : the current primary ID
Args : none
Status : public
This method gets or sets the primary ID of the feature in the
underlying Bio::DB::SeqFeature::Store database. If you change this
field and then call update(), it will have the effect of making a copy
of the feature in the database under a new ID.
 Title   : target
Usage : $segment = $feature->target
Function: return the segment correspondent to the "Target" attribute
Returns : a Bio::DB::SeqFeature::Segment object
Args : none
Status : public
For features that are aligned with others via the GFF3 Target
attribute, this returns a segment corresponding to the aligned
region. The CIGAR gap string is not yet supported.
Internal methodsTop
    Internal method used to implement overloaded stringification.
    $boolean = $feature->type_match(@list_of_types)
    Internal method that will return true if the primary_tag of the feature and
source_tag match any of the list of types (in primary_tag:source_tag
format) provided.
This is an early version, so there are certainly some bugs. Please
use the BioPerl bug tracking system to report bugs.
Lincoln Stein <>.
Copyright (c) 2006 Cold Spring Harbor Laboratory.
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.