Bio::Index Fastq
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Bio::Index::Fastq - Interface for indexing (multiple) fastq files
Package variables
No package variables defined.
Included modules
    # Complete code for making an index for several
# fastq files
use Bio::Index::Fastq;
use strict;
my $Index_File_Name = shift; my $inx = Bio::Index::Fastq->new( '-filename' => $Index_File_Name, '-write_flag' => 1); $inx->make_index(@ARGV); # Print out several sequences present in the index # in Fastq format use Bio::Index::Fastq; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Fastq->new('-filename' => $Index_File_Name); my $out = Bio::SeqIO->new('-format' => 'Fastq','-fh' => \*STDOUT); foreach my $id (@ARGV) { my $seq = $inx->fetch($id); # Returns Bio::Seq::Quality object $out->write_seq($seq); } # or, alternatively my $id; my $seq = $inx->get_Seq_by_id($id); #identical to fetch
Inherits functions for managing dbm files from,
and provides the basic funtionallity for indexing fastq files, and
retrieving the sequence from them. Note: for best results 'use strict'.
Bio::Index::Fastq supports the Bio::DB::BioSeqI interface, meaning
it can be used as a Sequence database for other parts of bioperl
No description
Methods description
_file_formatcode    nextTop
 Title   : _file_format
Function: The file format for this package, which is needed
by the SeqIO system when reading the sequence.
Returns : 'Fastq'
  Title   : _index_file
Usage : $index->_index_file( $file_name, $i )
Function: Specialist function to index FASTQ format files.
Is provided with a filename and an integer
by make_index in its SUPER class.
Example :
Returns :
Args :
  Title   : id_parser
Usage : $index->id_parser( CODE )
Function: Stores or returns the code used by record_id to
parse the ID for record from a string. Useful
for (for instance) specifying a different
parser for different flavours of FASTQ file.
Returns \&default_id_parser (see below) if not
set. If you supply your own id_parser
subroutine, then it should expect a fastq
description line. An entry will be added to
the index for each string in the list returned.
Example : $index->id_parser( \&my_id_parser )
Returns : ref to CODE if called without arguments
Args : CODE
  Title   : default_id_parser
Usage : $id = default_id_parser( $header )
Function: The default Fastq ID parser for
Returns $1 from applying the regexp /^>\s*(\S+)/
to $header.
Returns : ID string
Args : a fastq header line string
Methods code
sub _version {
    return 0.2;
sub _file_format {
    return 'Fastq';
sub _index_file {
    my( $self,
        $file, # File name
$i, # Index-number of file being indexed
) = @_; my( $begin, # Offset from start of file of the start
# of the last found record.
); $begin = 0; my $id_parser = $self->id_parser; my $c = 0; open my $FASTQ, '<', $file or $self->throw("Can't open file for read : $file"); # Main indexing loop
while (<$FASTQ>) { if (/^@/) { my $begin = tell($FASTQ) - length( $_ ); foreach my $id (&$id_parser($_)) { $self->add_record($id, $i, $begin); $c++; } } } close $FASTQ; return ($c);
sub id_parser {
    my( $self, $code ) = @_;
    if ($code) {
        $self->{'_id_parser'} = $code;
    return $self->{'_id_parser'} ||\& default_id_parser;
sub default_id_parser {
        if ($_[0] =~ /^@\s*(\S+)/) {
        return $1;
    } else {

General documentation
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.                  - General discussion - About the mailing lists
Support Top
Please direct usage questions or support issues to the mailing list:
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
AUTHOR - Tony CoxTop
Email -
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _