Bio::SearchIO
wise
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Summary
Bio::SearchIO::wise - Parsing of wise output as alignments
Package variables
No package variables defined.
Included modules
Inherit
Synopsis
use Bio::SearchIO;
my $parser = Bio::SearchIO->new(-file => 'file.genewise',
-format => 'wise',
-wisetype=> 'genewise');
while( my $result = $parser->next_result ) {}
Description
This object parsers Wise output using Bio::Tools::Genewise or
Bio::Tools::Genomewise as a helper.
Methods
Methods description
Title : next_result Usage : my $hit = $searchio->next_result; Function: Returns the next Result from a search Returns : Bio::Search::Result::ResultI object Args : none |
Title : start_element Usage : $eventgenerator->start_element Function: Handles a start element event Returns : none Args : hashref with at least 2 keys 'Data' and 'Name' |
Title : start_element Usage : $eventgenerator->end_element Function: Handles an end element event Returns : none Args : hashref with at least 2 keys 'Data' and 'Name' |
Title : element Usage : $eventhandler->element({'Name' => $name, 'Data' => $str}); Function: Convience method that calls start_element, characters, end_element Returns : none Args : Hash ref with the keys 'Name' and 'Data' |
Title : characters Usage : $eventgenerator->characters($str) Function: Send a character events Returns : none Args : string |
Title : within_element Usage : if( $eventgenerator->within_element($element) ) {} Function: Test if we are within a particular element This is different than 'in' because within can be tested for a whole block. Returns : boolean Args : string element name |
Title : in_element Usage : if( $eventgenerator->in_element($element) ) {} Function: Test if we are in a particular element This is different than 'in' because within can be tested for a whole block. Returns : boolean Args : string element name |
Title : start_document Usage : $eventgenerator->start_document Function: Handle a start document event Returns : none Args : none |
Title : end_document Usage : $eventgenerator->end_document Function: Handles an end document event Returns : Bio::Search::Result::ResultI object Args : none |
Title : wise Usage : $obj->wise($newval) Function: Get/Set the Wise object parser Returns : value of wise (a scalar) Args : on set, new value (a scalar or undef, optional) |
Title : wisetype Usage : $obj->wisetype($newval) Function: Wise program type Returns : value of wisetype (a scalar) Args : on set, new value (a scalar or undef, optional) |
Methods code
sub _initialize
{ my ($self,@args) = @_;
my ( $wisetype, $file,$fh ) =
$self->_rearrange([qw(WISETYPE FILE FH)], @args);
my @newargs;
while( @args ) {
my $a = shift @args;
if( $a =~ /FILE|FH/i ) {
shift @args;
next;
}
push @newargs, $a, shift @args;
}
$self->SUPER::_initialize(@newargs);
$self->{'_handler_cache'} = $self->_eventHandler;
$self->wisetype($wisetype);
my @ioargs;
if( $fh ) {
push @ioargs, ('-fh' => $fh);
} elsif( $file ) {
push @ioargs, ('-file' => $file);
}
if( $wisetype =~ /genewise/i ) {
$self->wise(Bio::Tools::Genewise->new(@ioargs));
} elsif( $wisetype =~ /genomewise/i ) {
$self->wise(Bio::Tools::Genomewise->new(@ioargs));
} else {
$self->throw("Must supply a -wisetype to ".ref($self)." which is one of 'genomewise' 'genewise'\n");
}
return $self;} |
sub next_result
{ my ($self) = @_;
local $/ = "\n";
local $_;
return unless $self->wise;
my $prediction = $self->wise->next_prediction;
return unless $prediction;
$self->{'_reporttype'} = uc $self->wisetype;
$self->start_element({'Name' => 'WiseOutput'});
$self->element({'Name' => 'WiseOutput_program',
'Data' => $self->wisetype});
$self->element({'Name' => 'WiseOutput_query-def',
'Data' => $self->wise->_prot_id});
my @transcripts = $prediction->transcripts;
foreach my $transcript ( @transcripts ) {
my @exons = $transcript->exons;
my $protid;
$self->start_element({'Name' => 'Hit'});
if( $exons[0]->has_tag('supporting_feature') ) {
my ($supporting_feature) = $exons[0]->get_tag_values('supporting_feature');
$protid = $supporting_feature->feature2->seq_id;
$self->element({'Name' => 'Hit_id',
'Data' => $self->wise->_target_id});
}
$self->element({'Name' => 'Hit_score',
'Data' => $self->wise->_score});
foreach my $exon ( @exons ) {
$self->start_element({'Name' => 'Hsp'});
if( $exon->strand < 0 ) {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->end});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->start});
} else {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->start});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->end});
}
$self->element({'Name' => 'Hsp_score',
'Data' => $self->wise->_score});
if( $exon->has_tag('supporting_feature') ) {
my ($sf) = $exon->get_tag_values('supporting_feature');
my $protein = $sf->feature2;
if( $protein->strand < 0 ) {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->end});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->start});
} else {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->start});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->end});
}
}
$self->element({'Name' => 'Hsp_identity',
'Data' => 0});
$self->element({'Name' => 'Hsp_positive',
'Data' => 0});
$self->end_element({'Name' => 'Hsp'});
}
$self->end_element({'Name' => 'Hit'});
}
$self->end_element({'Name' => 'WiseOutput'});
return $self->end_document();} |
sub start_element
{ my ($self,$data) = @_;
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
if( $type ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("start_%s",lc $type);
$self->_eventHandler->$func($data->{'Attributes'});
}
unshift @{$self->{'_elements'}}, $type;
if($type eq 'result') {
$self->{'_values'} = {};
$self->{'_result'}= undef;
}
} } |
sub end_element
{ my ($self,$data) = @_;
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
my $rc;
if( $type = $MODEMAP{$nm} ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("end_%s",lc $type);
$rc = $self->_eventHandler->$func($self->{'_reporttype'},
$self->{'_values'});
}
shift @{$self->{'_elements'}};
} elsif( $MAPPING{$nm} ) {
if ( ref($MAPPING{$nm}) =~ /hash/i ) {
my $key = (keys %{$MAPPING{$nm}})[0];
$self->{'_values'}->{$key}->{$MAPPING{$nm}->{$key}} = $self->{'_last_data'};
} else {
$self->{'_values'}->{$MAPPING{$nm}} = $self->{'_last_data'};
}
} else {
$self->debug( "unknown nm $nm, ignoring\n");
}
$self->{'_last_data'} = ''; $self->{'_result'} = $rc if( defined $type && $type eq 'result' );
return $rc;} |
sub element
{ my ($self,$data) = @_;
$self->start_element($data);
$self->characters($data);
$self->end_element($data); } |
sub characters
{ my ($self,$data) = @_;
return unless ( defined $data->{'Data'} && $data->{'Data'} !~ /^\s+$/ );
$self->{'_last_data'} = $data->{'Data'};} |
sub within_element
{ my ($self,$name) = @_;
return 0 if ( ! defined $name &&
! defined $self->{'_elements'} ||
scalar @{$self->{'_elements'}} == 0) ;
foreach ( @{$self->{'_elements'}} ) {
if( $_ eq $name ) {
return 1;
}
}
return 0;} |
sub in_element
{ my ($self,$name) = @_;
return 0 if ! defined $self->{'_elements'}->[0];
return ( $self->{'_elements'}->[0] eq $name)} |
sub start_document
{ my ($self) = @_;
$self->{'_lasttype'} = '';
$self->{'_values'} = {};
$self->{'_result'}= undef;
$self->{'_elements'} = [];
$self->{'_reporttype'} = 'exonerate';} |
sub end_document
{ my ($self,@args) = @_;
return $self->{'_result'};} |
sub write_result
{ my ($self, $blast, @args) = @_;
if( not defined($self->writer) ) {
$self->warn("Writer not defined. Using a $DEFAULT_WRITER_CLASS");
$self->writer( $DEFAULT_WRITER_CLASS->new() );
}
$self->SUPER::write_result( $blast, @args );} |
sub result_count
{ my $self = shift;
return $self->{'_result_count'};} |
sub wise
{ my $self = shift;
return $self->{'wise'} = shift if @_;
return $self->{'wise'};} |
sub wisetype
{ my $self = shift;
return $self->{'wisetype'} = shift if @_;
return $self->{'wisetype'};
}
1;} |
General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
https://redmine.open-bio.org/projects/bioperl/
| AUTHOR - Jason Stajich | Top |
Email jason-at-bioperl-dot-org
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
Title : new
Usage : my $obj = Bio::SearchIO::wise->new();
Function: Builds a new Bio::SearchIO::wise object
Returns : an instance of Bio::SearchIO::wise
Args : -wise => a Bio::Tools::Genewise or Bio::Tools::Genomewise object