Bio::Tools RNAMotif
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Summary
Bio::Tools::RNAMotif - A parser for RNAMotif output
Package variables
No package variables defined.
Included modules
Bio::SeqFeature::Collection
Bio::SeqFeature::Generic
Inherit
Bio::Tools::AnalysisResult
Synopsis
  use Bio::Tools::RNAMotif;
my $parser = Bio::Tools::RNAMotif->new(-file => $rna_output,
-motiftag => 'protein_bind'
-desctag => 'TRAP_binding');
#parse the results
while( my $motif = $parser->next_prediction) {
# do something here
}
Description
Parses raw RNAMotif output. RNAMotif uses a RNA profile, consisting
of sequence and structural elements stored in a descriptor file, to
search for potential motifs in a DNA sequence file. For more
information, see:
Macke TJ, Ecker DJ, Gutell RR, Gautheret D, Case DA, Sampath R.
RNAMotif, an RNA secondary structure definition and search algorithm.
Nucleic Acids Res. 2001 Nov 15;29(22):4724-35.
http://www.scripps.edu/mb/case/casegr-sh-3.5.html.
This module is not currently complete. As is, it will parse raw
RNAMotif output (i.e. information not passed through the secondary
programs rmfmt or rm2ct) and pack information into
Bio::SeqFeature::Generic objects. Currently, parsing extra output
utilized by the sprintf() function in an RNAMotif descriptor is not
implemented; this information is instead packed into the score tag,
which can be accessed by using the following:
  my ($score) = $feature->score; 
If the score contains anything besides a digit, it will throw a
warning that sprintf() may have been used.
Several values have also been added in the 'tag' hash. These can be
accessed using the following syntax:
  my ($entry) = $feature->get_Annotations('secstructure');
Added tags are :
   descline     - entire description line (in case the regex used for
sequence ID doesn't adequately catch the name
descfile - name of the descriptor file (may include path to file)
secstrucure - contains structural information from the descriptor
used as a query
sequence - sequence of motif, separated by spaces according to
matches to the structure in the descriptor (in
SecStructure).
See t/RNAMotif.t for example usage.
The clean_features method can also be used to return a list of seqfeatures (in a
Bio::SeqFeature::Collection object) that are within a particular region. RNAMotif
is prone with some descriptors to returning redundant hits; an attempt to rectify
this problem is attempted with RNAMotif's companion program rmprune, which returns
the structure with the longest helices (and theoretically the best scoring structure).
However, this doesn't take into account alternative foldings which may score better.
This method adds a bit more flexibility, giving the user the ability to screen folds
based on where the feature is found and the score. Passing a positive integer x
screens SeqFeatures based on the highest score within x bp, while a negative integer
screens based on the lowest score. So, to return the highest scoring values within
20 bp (likely using an arbitrary scroing system in the SCORE section of a descriptor
file), one could use:
  $list = $obj->clean_features(20); 
... and returning the lowest scoring structures within the same region (when the
score is based on calculated free energies from efn2) can be accomplished
by the following:
  $list = $obj->clean_features(-20);
If you wanted the best feature in a sequence, you could set this to a large number,
preferrably on that exceeds the bases in a sequence
  $list = $obj->clean_features(10000000);
Each seqfeature in the collection can then be acted upon:
  @sf = $list->get_all_features;
for my $f (@sf) {
# do crazy things here
}
At some point a more complicated feature object may be used to support
this data rather than forcing most of the information into tag/value
pairs in a SeqFeature::Generic. This will hopefully allow for more
flexible analysis of data (specifically RNA secondary structural
data). It works for now...
Methods
_initialize
No description
Code
motif_tagDescriptionCode
source_tagDescriptionCode
desc_tagDescriptionCode
analysis_methodDescriptionCode
next_featureDescriptionCode
next_predictionDescriptionCode
clean_featuresDescriptionCode
Methods description
motif_tagcode    nextTop
 Title   : motif_tag
Usage : $obj->motif_tag($newval)
Function: Get/Set the value used for 'motif_tag', which is used for setting the
primary_tag.
Default is 'misc_binding' as set by the global $MotifTag.
'misc_binding' is used here because a conserved RNA motif is capable
of binding proteins (regulatory proteins), antisense RNA (siRNA),
small molecules (riboswitches), or nothing at all (tRNA,
terminators, etc.). It is recommended that this be changed to other
tags ('misc_RNA', 'protein_binding', 'tRNA', etc.) where appropriate.
For more information, see:
http://www.ncbi.nlm.nih.gov/collab/FT/index.html
Returns : value of motif_tag (a scalar)
Args : on set, new value (a scalar or undef, optional)
source_tagcodeprevnextTop
 Title   : source_tag
Usage : $obj->source_tag($newval)
Function: Get/Set the value used for the 'source_tag'.
Default is 'RNAMotif' as set by the global $SrcTag
Returns : value of source_tag (a scalar)
Args : on set, new value (a scalar or undef, optional)
desc_tagcodeprevnextTop
 Title   : desc_tag
Usage : $obj->desc_tag($newval)
Function: Get/Set the value used for the query motif. This will be placed in
the tag '-display_name'. Default is 'rnamotif' as set by the global
$DescTag. Use this to manually set the descriptor (motif searched for).
Since there is no way for this module to tell what the motif is from the
name of the descriptor file or the RNAMotif output, this should
be set every time an RNAMotif object is instantiated for clarity
Returns : value of exon_tag (a scalar)
Args : on set, new value (a scalar or undef, optional)
analysis_methodcodeprevnextTop
 Usage     : $obj->analysis_method();
Purpose : Inherited method. Overridden to ensure that the name matches
/RNAMotif/i.
Returns : String
Argument : n/a
next_featurecodeprevnextTop
 Title   : next_feature
Usage : while($gene = $obj->next_feature()) {
# do something
}
Function: Returns the next gene structure prediction of the RNAMotif result
file. Call this method repeatedly until FALSE is returned.
The returned object is actually a SeqFeatureI implementing object.
This method is required for classes implementing the
SeqAnalysisParserI interface, and is merely an alias for
next_prediction() at present.
Returns : A Bio::Tools::Prediction::Gene object.
Args : None (at present)
next_predictioncodeprevnextTop
 Title   : next_prediction
Usage : while($gene = $obj->next_prediction()) {
# do something
}
Function: Returns the next gene structure prediction of the RNAMotif result
file. Call this method repeatedly until FALSE is returned.
Returns : A Bio::SeqFeature::Generic object
Args : None (at present)
clean_featurescodeprevnextTop
 Title   : next_prediction
Usage : @list = $obj->clean_features(-10);
Function: Cleans (reduces redundant hits) based on score, position
Returns : a Bio::SeqFeature::Collection object
Args : Pos./Neg. integer (for highest/lowest scoring seqfeature within x bp).
Throws : Error unless digit is entered.
Methods code
_initializedescriptionprevnextTop
sub _initialize {
  my($self,@args) = @_;
  $self->warn('Use of this module is deprecated.  Use Bio::SearchIO::rnamotif instead');
  $self->SUPER::_initialize(@args);
  my ($motiftag,$desctag,$srctag) =  $self->SUPER::_rearrange([qw(MOTIFTAG
                                                                  DESCTAG
                                                                  SRCTAG
                                 )],
                                  @args);
  $self->motif_tag(defined $motiftag ? $motiftag : $MotifTag);
  $self->source_tag(defined $srctag ? $srctag : $SrcTag);
  $self->desc_tag(defined $desctag ? $desctag : $DescTag);
  $self->{'_sec_structure' => '',
          '_dfile' => ''};
}
motif_tagdescriptionprevnextTop
sub motif_tag {
    my $self = shift;

    return $self->{'motif_tag'} = shift if @_;
    return $self->{'motif_tag'};
}
source_tagdescriptionprevnextTop
sub source_tag {
    my $self = shift;

    return $self->{'source_tag'} = shift if @_;
    return $self->{'source_tag'};
}
desc_tagdescriptionprevnextTop
sub desc_tag {
    my $self = shift;

    return $self->{'desc_tag'} = shift if @_;
    return $self->{'desc_tag'};
}
analysis_methoddescriptionprevnextTop
sub analysis_method {
 #-------------
my ($self, $method) = @_; if($method && ($method !~ /RNAMotif/i)) { $self->throw("method $method not supported in " . ref($self)); } return $self->SUPER::analysis_method($method);
}
next_featuredescriptionprevnextTop
sub next_feature {
    my ($self,@args) = @_;
    # even though next_prediction doesn't expect any args (and this method
# does neither), we pass on args in order to be prepared if this changes
# ever
return $self->next_prediction(@args);
}
next_predictiondescriptionprevnextTop
sub next_prediction {
    my ($self) = @_;
    my ($motiftag,$srctag,$desctag) = ( $self->motif_tag,
                       $self->source_tag,
                       $self->desc_tag);
    my ($score, $strand, $start, $length, $sequence, $end, $seqid, $description)=0;
    while($_ = $self->_readline) {
        while($_ =~ /^#RM/) { # header line
if(/^#RM\sdescr\s(.*)$/) { # contains sec structure
$self->{'_sec_structure'}=$1; } if(/^#RM\sdfile\s(.*)$/) { # contains dfile
$self->{'_dfile'}=$1; } $_ = $self->_readline; } if(m/^>((\S*)\s.*)$/) {
$seqid = $2;
$description = $1; # contains entire description line if needed
if($seqid =~ /(gb|emb|dbj|sp|pdb|bbs|ref|lcl)\|(.*)\|/) { $seqid = $2; # pulls out gid
} } # start pulling out hit information...
# regex is m/^\S+\s+(\S+)\s+(\d+)\s+(\d+)\s+(\d+)\s(.*)$/
# m/^\S+\s+ # seqID, not needed
# (.+)\s+ # score, or extra info using sprintf()
# (\d+)\s+ # strand
# (\d+)\s+ # start
# (\d+)\s # length
# (.*)$/ # sequence, divided according to descriptor
if(m/^\S+\s+(.+)\s+(\d+)\s+(\d+)\s+(\d+)\s(.*)$/) {
($score, $strand, $start, $length, $sequence, $end)=
($1, $2, $3, $4, $5, 0);
if( $strand==0 ) { $end = $start + $length -1; $strand = 1; } else { $end = $start - $length + 1; ($start, $end, $strand) = ($end, $start, -1); } my $gene = Bio::SeqFeature::Generic->new(-seq_id => $seqid, -start => $start, -end => $end, -strand => $strand, -score => $score, -primary_tag => $motiftag, -source_tag => $srctag, -display_name => $desctag, -tag => { 'descline' => $description, 'descfile' => $self->{'_dfile'}, 'secstructure' => $self->{'_sec_structure'}, 'sequence' => $sequence}); return $gene; } }
}
clean_featuresdescriptionprevnextTop
sub clean_features {
    my $self = shift;
    my $bp = shift;
    $self->throw("No arg, need pos. or neg. integer") if !$bp;
    $self->throw("Need pos. or neg. integer") if ($bp !~ /\-?\d/ || $bp =~ /\./);
    my ($b, $sf2);
    my @list = ();
    my @features = ();
    while (my $pred = $self->next_prediction) {
        push @features, $pred;
    }
    while (@features) {
        $b = shift @features if !defined($b);
        $sf2 = shift @features;
        # from same sequence?
if ($sf2) { # if there is no feature, then...
if ($b->seq_id == $sf2->seq_id) { # close starts (probable redundant hit)?
if(abs(($b->start)-($sf2->start)) <= abs($bp)) { # which score is better?
if( (($bp < 0) && ($b->score > $sf2->score)) || # lowest score
(($bp > 0) && ($b->score < $sf2->score)) ){ # highest score
$b = $sf2; next; } else { next; } } push @list, $b; $b = $sf2; } } } push @list, $b if $b; my $col = Bio::SeqFeature::Collection->new; $col->add_features(\@list); return $col; } 1;
}
General documentation
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
  bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support Top
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
  https://redmine.open-bio.org/projects/bioperl/
AUTHOR - Chris FieldsTop
Email cjfields-at-uiuc-dot-edu
APPENDIXTop
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
newTop
 Title   : new
Usage : my $obj = Bio::Tools::RNAMotif->new();
Function: Builds a new Bio::Tools::RNAMotif object
Returns : an instance of Bio::Tools::RNAMotif
Args : -fh/-file for input filename
-motiftag => primary tag used in gene features (default 'misc_binding')
-desctag => tag used for display_name name (default 'rnamotif')
-srctag => source tag used in all features (default 'RNAMotif')