Bio::Tools SeqStats
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Summary
Bio::Tools::SeqStats - Object holding statistics for one
particular sequence
Package variables
No package variables defined.
Included modules
Bio::Seq
Inherit
Bio::Root::Root
Synopsis
  # build a primary nucleic acid or protein sequence object somehow
# then build a statistics object from the sequence object
$seqobj = Bio::PrimarySeq->new(-seq => 'ACTGTGGCGTCAACTG', -alphabet => 'dna', -id => 'test'); $seq_stats = Bio::Tools::SeqStats->new(-seq => $seqobj); # obtain a hash of counts of each type of monomer # (i.e. amino or nucleic acid) print "\nMonomer counts using statistics object\n"; $seq_stats = Bio::Tools::SeqStats->new(-seq=>$seqobj); $hash_ref = $seq_stats->count_monomers(); # e.g. for DNA sequence foreach $base (sort keys %$hash_ref) { print "Number of bases of type ", $base, "= ", %$hash_ref->{$base},"\n"; } # obtain the count directly without creating a new statistics object print "\nMonomer counts without statistics object\n"; $hash_ref = Bio::Tools::SeqStats->count_monomers($seqobj); foreach $base (sort keys %$hash_ref) { print "Number of bases of type ", $base, "= ", %$hash_ref->{$base},"\n"; } # obtain hash of counts of each type of codon in a nucleic acid sequence print "\nCodon counts using statistics object\n"; $hash_ref = $seq_stats-> count_codons(); # for nucleic acid sequence foreach $base (sort keys %$hash_ref) { print "Number of codons of type ", $base, "= ", %$hash_ref->{$base},"\n"; } # or print "\nCodon counts without statistics object\n"; $hash_ref = Bio::Tools::SeqStats->count_codons($seqobj); foreach $base (sort keys %$hash_ref) { print "Number of codons of type ", $base, "= ", %$hash_ref->{$base},"\n"; } # Obtain the molecular weight of a sequence. Since the sequence # may contain ambiguous monomers, the molecular weight is returned # as a (reference to) a two element array containing greatest lower # bound (GLB) and least upper bound (LUB) of the molecular weight $weight = $seq_stats->get_mol_wt(); print "\nMolecular weight (using statistics object) of sequence ", $seqobj->id(), " is between ", $$weight[0], " and " , $$weight[1], "\n"; # or $weight = Bio::Tools::SeqStats->get_mol_wt($seqobj); print "\nMolecular weight (without statistics object) of sequence ", $seqobj->id(), " is between ", $$weight[0], " and " , $$weight[1], "\n"; # Calculate mean Kyte-Doolittle hydropathicity (aka "gravy" score) my $prot = Bio::PrimarySeq->new(-seq=>'MSFVLVAPDMLATAAADVVQIGSAVSAGS', -alphabet=>'protein'); my $gravy = Bio::Tools::SeqStats->hydropathicity($seqobj); print "might be hydropathic" if $gravy > 1;
Description
Bio::Tools::SeqStats is a lightweight object for the calculation of
simple statistical and numerical properties of a sequence. By
"lightweight" I mean that only "primary" sequences are handled by the
object. The calling script needs to create the appropriate primary
sequence to be passed to SeqStats if statistics on a sequence feature
are required. Similarly if a codon count is desired for a
frame-shifted sequence and/or a negative strand sequence, the calling
script needs to create that sequence and pass it to the SeqStats
object.
Nota that nucleotide sequences in bioperl do not strictly separate RNA
and DNA sequences. By convention, sequences from RNA molecules are
shown as is they were DNA. Objects are supposed to make the
distinction when needed. This class is one of the few where this
distinctions needs to be made. Internally, it changes all Ts into Us
before weight and monomer count.
SeqStats can be called in two distinct manners. If only a single
computation is required on a given sequence object, the method can be
called easily using the SeqStats object directly:
  $weight = Bio::Tools::SeqStats->get_mol_wt($seqobj);
Alternately, if several computations will be required on a given
sequence object, an "instance" statistics object can be constructed
and used for the method calls:
  $seq_stats = Bio::Tools::SeqStats->new($seqobj);
$monomers = $seq_stats->count_monomers();
$codons = $seq_stats->count_codons();
$weight = $seq_stats->get_mol_wt();
$gravy = $seq_stats->hydropathicity();
As currently implemented the object can return the following values
from a sequence:
    *(1)
    The molecular weight of the sequence: get_mol_wt()
    *(2)
    The number of each type of monomer present: count_monomers()
    *(3)
    The number of each codon present in a nucleic acid sequence:
count_codons()
    *(4)
    The mean hydropathicity ("gravy" score) of a protein:
hydropathicity()
For DNA and RNA sequences single-stranded weights are returned. The
molecular weights are calculated for neutral, or not ionized,
nucleic acids. The returned weight is the sum of the
base-sugar-phosphate residues of the chain plus one weight of water to
to account for the additional OH on the phosphate of the 5' residue
and the additional H on the sugar ring of the 3' residue. Note that
this leads to a difference of 18 in calculated molecular weights
compared to some other available programs (e.g. Informax VectorNTI).
Note that since sequences may contain ambiguous monomers (e.g. "M",
meaning "A" or "C" in a nucleic acid sequence), the method get_mol_wt
returns a two-element array containing the greatest lower bound and
least upper bound of the molecule. For a sequence with no ambiguous
monomers, the two elements of the returned array will be equal. The
method count_codons() handles ambiguous bases by simply counting all
ambiguous codons together and issuing a warning to that effect.
Methods
BEGIN Code
new
No description
Code
count_monomersDescriptionCode
get_mol_wtDescriptionCode
count_codonsDescriptionCode
hydropathicityDescriptionCode
_is_alphabet_strictDescriptionCode
_print_dataDescriptionCode
Methods description
count_monomerscode    nextTop
 Title   : count_monomers
Usage : $rcount = $seq_stats->count_monomers();
or $rcount = $seq_stats->Bio::Tools::SeqStats->($seqobj);
Function: Counts the number of each type of monomer (amino acid or
base) in the sequence.
Ts are counted as Us in RNA sequences.
Example :
Returns : Reference to a hash in which keys are letters of the
genetic alphabet used and values are number of occurrences
of the letter in the sequence.
Args : None or reference to sequence object
Throws : Throws an exception if type of sequence is unknown (ie amino
or nucleic)or if unknown letter in alphabet. Ambiguous
elements are allowed.
get_mol_wtcodeprevnextTop
 Title   : get_mol_wt
Usage : $wt = $seqobj->get_mol_wt() or
$wt = Bio::Tools::SeqStats ->get_mol_wt($seqobj);
Function: Calculate molecular weight of sequence
Ts are counted as Us in RNA sequences.
Example :
Returns : Reference to two element array containing lower and upper bounds of molecule molecular weight. For DNA and RNA sequences single-stranded weights are returned. If sequence contains no ambiguous elements, both entries in array are equal to molecular weight of molecule. Args : None or reference to sequence object Throws : Exception if type of sequence is unknown (ie not amino or nucleic) or if unknown letter in alphabet. Ambiguous elements are allowed.
count_codonscodeprevnextTop
 Title   : count_codons
Usage : $rcount = $seqstats->count_codons() or
$rcount = Bio::Tools::SeqStats->count_codons($seqobj)
Function: Counts the number of each type of codons for a dna or rna
sequence, starting at the 1st triple of the input sequence.
Example :
Returns : Reference to a hash in which keys are codons of the genetic
alphabet used and values are number of occurrences of the
codons in the sequence. All codons with "ambiguous" bases
are counted together.
Args : None or sequence object
Throws : an exception if type of sequence is unknown or protein.
hydropathicitycodeprevnextTop
 Title   : hydropathicity
Usage : $gravy = $seqstats->hydropathicity(); or
$gravy = Bio::Tools::SeqStats->hydropathicity($seqobj);
Function: Calculates the mean Kyte-Doolittle hydropathicity for a protein sequence. Also known as the "gravy" score. Refer to Kyte J., Doolittle R.F., J. Mol. Biol. 157:105-132(1982). Example : Returns : float Args : None or reference to sequence object Throws : an exception if type of sequence is not protein.
_is_alphabet_strictcodeprevnextTop
 Title   :  _is_alphabet_strict
Usage :
Function: internal function to determine whether there are
any ambiguous elements in the current sequence
Example :
Returns : 1 if strict alphabet is being used,
0 if ambiguous elements are present
Args :
Throws : an exception if type of sequence is unknown (ie amino or nucleic) or if unknown letter in alphabet. Ambiguous monomers are allowed.
_print_datacodeprevnextTop
 Title   : _print_data
Usage : $seqobj->_print_data() or Bio::Tools::SeqStats->_print_data();
Function: Displays dna / rna parameters (used for debugging)
Returns : 1
Args : None
Used for debugging.
Methods code
BEGINTop
BEGIN {
	%Alphabets =   (
			 'dna'     => [ qw(A C G T R Y M K S W H B V D X N) ],
		    'rna'     => [ qw(A C G U R Y M K S W H B V D X N) ],
		    'protein' => [ qw(A R N D C Q E G H I L K M F U
									 P S T W X Y V B Z J O *) ], # sac: added B, Z
); # SAC: new strict alphabet: doesn't allow any ambiguity characters.
%Alphabets_strict = ( 'dna' => [ qw( A C G T ) ], 'rna' => [ qw( A C G U ) ], 'protein' => [ qw(A R N D C Q E G H I L K M F U P S T W Y V O) ], ); # IUPAC-IUB SYMBOLS FOR NUCLEOTIDE NOMENCLATURE:
# Cornish-Bowden (1985) Nucl. Acids Res. 13: 3021-3030.
# Amino Acid alphabet
# ------------------------------------------
# Symbol Meaning
# ------------------------------------------
my $amino_A_wt = 89.09; my $amino_C_wt = 121.15; my $amino_D_wt = 133.1; my $amino_E_wt = 147.13; my $amino_F_wt = 165.19; my $amino_G_wt = 75.07; my $amino_H_wt = 155.16; my $amino_I_wt = 131.17; my $amino_K_wt = 146.19; my $amino_L_wt = 131.17; my $amino_M_wt = 149.21; my $amino_N_wt = 132.12; my $amino_O_wt = 255.31; my $amino_P_wt = 115.13; my $amino_Q_wt = 146.15; my $amino_R_wt = 174.20; my $amino_S_wt = 105.09; my $amino_T_wt = 119.12; my $amino_U_wt = 168.06; my $amino_V_wt = 117.15; my $amino_W_wt = 204.23; my $amino_Y_wt = 181.19; $amino_weights = { 'A' => [$amino_A_wt, $amino_A_wt], # Alanine
'B' => [$amino_N_wt, $amino_D_wt], # Aspartic Acid, Asparagine
'C' => [$amino_C_wt, $amino_C_wt], # Cysteine
'D' => [$amino_D_wt, $amino_D_wt], # Aspartic Acid
'E' => [$amino_E_wt, $amino_E_wt], # Glutamic Acid
'F' => [$amino_F_wt, $amino_F_wt], # Phenylalanine
'G' => [$amino_G_wt, $amino_G_wt], # Glycine
'H' => [$amino_H_wt, $amino_H_wt], # Histidine
'I' => [$amino_I_wt, $amino_I_wt], # Isoleucine
'J' => [$amino_L_wt, $amino_I_wt], # Leucine, Isoleucine
'K' => [$amino_K_wt, $amino_K_wt], # Lysine
'L' => [$amino_L_wt, $amino_L_wt], # Leucine
'M' => [$amino_M_wt, $amino_M_wt], # Methionine
'N' => [$amino_N_wt, $amino_N_wt], # Asparagine
'O' => [$amino_O_wt, $amino_O_wt], # Pyrrolysine
'P' => [$amino_P_wt, $amino_P_wt], # Proline
'Q' => [$amino_Q_wt, $amino_Q_wt], # Glutamine
'R' => [$amino_R_wt, $amino_R_wt], # Arginine
'S' => [$amino_S_wt, $amino_S_wt], # Serine
'T' => [$amino_T_wt, $amino_T_wt], # Threonine
'U' => [$amino_U_wt, $amino_U_wt], # SelenoCysteine
'V' => [$amino_V_wt, $amino_V_wt], # Valine
'W' => [$amino_W_wt, $amino_W_wt], # Tryptophan
'X' => [$amino_G_wt, $amino_W_wt], # Unknown
'Y' => [$amino_Y_wt, $amino_Y_wt], # Tyrosine
'Z' => [$amino_Q_wt, $amino_E_wt], # Glutamic Acid, Glutamine
}; # Extended Dna / Rna alphabet
use vars ( qw($C $O $N $H $P $water) ); use vars ( qw($adenine $guanine $cytosine $thymine $uracil)); use vars ( qw($ribose_phosphate $deoxyribose_phosphate $ppi)); use vars ( qw($dna_A_wt $dna_C_wt $dna_G_wt $dna_T_wt $rna_A_wt $rna_C_wt $rna_G_wt $rna_U_wt)); use vars ( qw($dna_weights $rna_weights %Weights)); $C = 12.01; $O = 16.00; $N = 14.01; $H = 1.01; $P = 30.97; $water = 18.015; $adenine = 5 * $C + 5 * $N + 5 * $H; $guanine = 5 * $C + 5 * $N + 1 * $O + 5 * $H; $cytosine = 4 * $C + 3 * $N + 1 * $O + 5 * $H; $thymine = 5 * $C + 2 * $N + 2 * $O + 6 * $H; $uracil = 4 * $C + 2 * $N + 2 * $O + 4 * $H; $ribose_phosphate = 5 * $C + 7 * $O + 9 * $H + 1 * $P; # neutral (unionized) form
$deoxyribose_phosphate = 5 * $C + 6 * $O + 9 * $H + 1 * $P; # the following are single strand molecular weights / base
$dna_A_wt = $adenine + $deoxyribose_phosphate - $water; $dna_C_wt = $cytosine + $deoxyribose_phosphate - $water; $dna_G_wt = $guanine + $deoxyribose_phosphate - $water; $dna_T_wt = $thymine + $deoxyribose_phosphate - $water; $rna_A_wt = $adenine + $ribose_phosphate - $water; $rna_C_wt = $cytosine + $ribose_phosphate - $water; $rna_G_wt = $guanine + $ribose_phosphate - $water; $rna_U_wt = $uracil + $ribose_phosphate - $water; $dna_weights = { 'A' => [$dna_A_wt,$dna_A_wt], # Adenine
'C' => [$dna_C_wt,$dna_C_wt], # Cytosine
'G' => [$dna_G_wt,$dna_G_wt], # Guanine
'T' => [$dna_T_wt,$dna_T_wt], # Thymine
'M' => [$dna_C_wt,$dna_A_wt], # A or C
'R' => [$dna_A_wt,$dna_G_wt], # A or G
'W' => [$dna_T_wt,$dna_A_wt], # A or T
'S' => [$dna_C_wt,$dna_G_wt], # C or G
'Y' => [$dna_C_wt,$dna_T_wt], # C or T
'K' => [$dna_T_wt,$dna_G_wt], # G or T
'V' => [$dna_C_wt,$dna_G_wt], # A or C or G
'H' => [$dna_C_wt,$dna_A_wt], # A or C or T
'D' => [$dna_T_wt,$dna_G_wt], # A or G or T
'B' => [$dna_C_wt,$dna_G_wt], # C or G or T
'X' => [$dna_C_wt,$dna_G_wt], # G or A or T or C
'N' => [$dna_C_wt,$dna_G_wt], # G or A or T or C
}; $rna_weights = { 'A' => [$rna_A_wt,$rna_A_wt], # Adenine
'C' => [$rna_C_wt,$rna_C_wt], # Cytosine
'G' => [$rna_G_wt,$rna_G_wt], # Guanine
'U' => [$rna_U_wt,$rna_U_wt], # Uracil
'M' => [$rna_C_wt,$rna_A_wt], # A or C
'R' => [$rna_A_wt,$rna_G_wt], # A or G
'W' => [$rna_U_wt,$rna_A_wt], # A or U
'S' => [$rna_C_wt,$rna_G_wt], # C or G
'Y' => [$rna_C_wt,$rna_U_wt], # C or U
'K' => [$rna_U_wt,$rna_G_wt], # G or U
'V' => [$rna_C_wt,$rna_G_wt], # A or C or G
'H' => [$rna_C_wt,$rna_A_wt], # A or C or U
'D' => [$rna_U_wt,$rna_G_wt], # A or G or U
'B' => [$rna_C_wt,$rna_G_wt], # C or G or U
'X' => [$rna_C_wt,$rna_G_wt], # G or A or U or C
'N' => [$rna_C_wt,$rna_G_wt], # G or A or U or C
}; %Weights = ( 'dna' => $dna_weights, 'rna' => $rna_weights, 'protein' => $amino_weights, ); # Amino acid scale: Hydropathicity.
# Ref: Kyte J., Doolittle R.F. J. Mol. Biol. 157:105-132(1982).
# http://au.expasy.org/tools/pscale/Hphob.Doolittle.html
$amino_hydropathicity = { A => 1.800, R => -4.500, N => -3.500, D => -3.500, C => 2.500, Q => -3.500, E => -3.500, G => -0.400, H => -3.200, I => 4.500, L => 3.800, K => -3.900, M => 1.900, F => 2.800, P => -1.600, S => -0.800, T => -0.700, W => -0.900, Y => -1.300, V => 4.200, };
}
newdescriptionprevnextTop
sub new {
	my($class,@args) = @_;
	my $self = $class->SUPER::new(@args);

	my ($seqobj) = $self->_rearrange([qw(SEQ)],@args);
	unless  ($seqobj->isa("Bio::PrimarySeqI")) {
		$self->throw("SeqStats works only on PrimarySeqI objects");
	}
	if ( !defined $seqobj->alphabet || 
		  !defined $Alphabets{$seqobj->alphabet}) {
		$self->throw("Must have a valid alphabet defined for seq (".
						 join(",",keys %Alphabets));
	}
	$self->{'_seqref'} = $seqobj;
	# check the letters in the sequence
$self->{'_is_strict'} = _is_alphabet_strict($seqobj); return $self;
}
count_monomersdescriptionprevnextTop
sub count_monomers {
	my %count  = ();
	my $seqobj;
	my $_is_strict;
	my $element = '';
	my $_is_instance = 1 ;
	my $self = shift @_;
	my $object_argument = shift @_;

	# First we need to determine if the present object is an instance
# object or if the sequence object has been passed as an argument
if (defined $object_argument) { $_is_instance = 0; } # If we are using an instance object...
if ($_is_instance) { if ($self->{'_monomer_count'}) { return $self->{'_monomer_count'}; # return count if previously calculated
} $_is_strict = $self->{'_is_strict'}; # retrieve "strictness"
$seqobj = $self->{'_seqref'}; } else { # otherwise...
$seqobj = $object_argument; # Following two lines lead to error in "throw" routine
$seqobj->isa("Bio::PrimarySeqI") || $self->throw("SeqStats works only on PrimarySeqI objects"); # is alphabet OK? Is it strict?
$_is_strict = _is_alphabet_strict($seqobj); } my $alphabet = $_is_strict ? $Alphabets_strict{$seqobj->alphabet} : $Alphabets{$seqobj->alphabet} ; # get array of allowed letters
# convert everything to upper case to be safe
my $seqstring = uc $seqobj->seq(); # Since T is used in RichSeq RNA sequences, do conversion locally
$seqstring =~ s/T/U/g if $seqobj->alphabet eq 'rna'; # For each letter, count the number of times it appears in
# the sequence
LETTER: foreach $element (@$alphabet) { # skip terminator symbol which may confuse regex
next LETTER if $element eq '*'; $count{$element} = ( $seqstring =~ s/$element/$element/g); } if ($_is_instance) { $self->{'_monomer_count'} =\% count; # Save in case called again later
} return\% count;
}
get_mol_wtdescriptionprevnextTop
sub get_mol_wt {
	my $seqobj;
	my $_is_strict;
	my $element = '';
	my $_is_instance = 1 ;
	my $self = shift @_;
	my $object_argument = shift @_;
	my ($weight_array, $rcount);

	if (defined $object_argument) {
		$_is_instance = 0;
	}

	if ($_is_instance) {
		if ($weight_array = $self->{'_mol_wt'}) {
			# return mol. weight if previously calculated
return $weight_array; } $seqobj = $self->{'_seqref'}; $rcount = $self->count_monomers(); } else { $seqobj = $object_argument; $seqobj->isa("Bio::PrimarySeqI") || $self->throw("Error: SeqStats works only on PrimarySeqI objects"); $_is_strict = _is_alphabet_strict($seqobj); # is alphabet OK?
$rcount = $self->count_monomers($seqobj); } # We will also need to know what type of monomer we are dealing with
my $moltype = $seqobj->alphabet(); # In general,the molecular weight is bounded below by the sum of the
# weights of lower bounds of each alphabet symbol times the number of
# occurrences of the symbol in the sequence. A similar upper bound on
# the weight is also calculated.
# Note that for "strict" (i.e. unambiguous) sequences there is an
# inefficiency since the upper bound = the lower bound and there are
# two calculations. However, this decrease in performance will be
# minor and leads to significantly more readable code.
my $weight_lower_bound = 0; my $weight_upper_bound = 0; my $weight_table = $Weights{$moltype}; my $total_res; # compute weight of all the residues
foreach $element (keys %$rcount) { $weight_lower_bound += $$rcount{$element} * $$weight_table{$element}->[0]; $weight_upper_bound += $$rcount{$element} * $$weight_table{$element}->[1]; # this tracks only the residues used for counting MW
$total_res += $$rcount{$element}; } if ($moltype =~ /protein/) { # remove H2O during peptide bond formation.
$weight_lower_bound -= $water * ($total_res - 1); $weight_upper_bound -= $water * ($total_res - 1); } else { # Correction because phosphate of 5' residue has additional OH and
# sugar ring of 3' residue has additional H
$weight_lower_bound += $water; $weight_upper_bound += $water; } $weight_lower_bound = sprintf("%.1f", $weight_lower_bound); $weight_upper_bound = sprintf("%.1f", $weight_upper_bound); $weight_array = [$weight_lower_bound, $weight_upper_bound]; if ($_is_instance) { $self->{'_mol_wt'} = $weight_array; # Save in case called again later
} return $weight_array;
}
count_codonsdescriptionprevnextTop
sub count_codons {
	my $rcount = {};
	my $codon ;
	my $seqobj;
	my $_is_strict;
	my $element = '';
	my $_is_instance = 1 ;
	my $self = shift @_;
	my $object_argument = shift @_;

	if (defined $object_argument) {
		$_is_instance = 0;
	}

	if ($_is_instance) {
		if ($rcount = $self->{'_codon_count'}) {
			return $rcount;        # return count if previously calculated
} $_is_strict = $self->{'_is_strict'}; # retrieve "strictness"
$seqobj = $self->{'_seqref'}; } else { $seqobj = $object_argument; $seqobj->isa("Bio::PrimarySeqI") || $self->throw("Error: SeqStats works only on PrimarySeqI objects"); $_is_strict = _is_alphabet_strict($seqobj); } # Codon counts only make sense for nucleic acid sequences
my $alphabet = $seqobj->alphabet(); unless ($alphabet =~ /[dr]na/i) { $seqobj->throw("Codon counts only meaningful for dna or rna, ". "not for $alphabet sequences."); } # If sequence contains ambiguous bases, warn that codons
# containing them will all be lumped together in the count.
if (!$_is_strict ) { $seqobj->warn("Sequence $seqobj contains ambiguous bases.". " All codons with ambiguous bases will be added together in count.") if $self->verbose >= 0 ; } my $seq = $seqobj->seq(); # Now step through the string by threes and count the codons
CODON: while (length($seq) > 2) { $codon = uc substr($seq,0,3); $seq = substr($seq,3); if ($codon =~ /[^ACTGU]/i) { $$rcount{'ambiguous'}++; #lump together ambiguous codons
next CODON; } if (!defined $$rcount{$codon}) { $$rcount{$codon}= 1 ; next CODON; } $$rcount{$codon}++; # default
} if ($_is_instance) { $self->{'_codon_count'} = $rcount; # Save in case called again later
} return $rcount;
}
hydropathicitydescriptionprevnextTop
sub hydropathicity {
	my $seqobj;
	my $_is_strict;
	my $element = '';
	my $_is_instance = 1 ;
	my $self = shift @_;
	my $object_argument = shift @_;

	if (defined $object_argument) {
		$_is_instance = 0;
	}

	if ($_is_instance) {
		if (my $gravy = $self->{'_hydropathicity'}) {
			return $gravy;        # return value if previously calculated
} $_is_strict = $self->{'_is_strict'}; # retrieve "strictness"
$seqobj = $self->{'_seqref'}; } else { $seqobj = $object_argument; $seqobj->isa("Bio::PrimarySeqI") || $self->throw("Error: SeqStats works only on PrimarySeqI objects"); $_is_strict = _is_alphabet_strict($seqobj); } # hydropathicity not menaingful for empty sequences
unless ($seqobj->length() > 0) { $seqobj->throw("hydropathicity not defined for zero-length sequences"); } # hydropathicity only make sense for protein sequences
my $alphabet = $seqobj->alphabet(); unless ($alphabet =~ /protein/i) { $seqobj->throw("hydropathicity only meaningful for protein, ". "not for $alphabet sequences."); } # If sequence contains ambiguous bases, warn that codons
# containing them will all be lumped together in the count.
unless ($_is_strict ) { $seqobj->throw("Sequence $seqobj contains ambiguous amino acids. ". "Hydropathicity can not be caculated.") } my $seq = $seqobj->seq(); # Now step through the string and add up the hydropathicity values
my $gravy = 0; for my $i ( 0 .. length($seq) ) { my $codon = uc(substr($seq,$i,1)); $gravy += $amino_hydropathicity->{$codon}||0; # table look-up
} $gravy /= length($seq);
if ($_is_instance) { $self->{'_hydropathicity'} = $gravy; # Save in case called again later
} return $gravy;
}
_is_alphabet_strictdescriptionprevnextTop
sub _is_alphabet_strict {
	my ($seqobj) = @_;
	my $moltype = $seqobj->alphabet();

	# convert everything to upper case to be safe
my $seqstring = uc $seqobj->seq(); # Since T is used in RichSeq RNA sequences, do conversion locally
$seqstring =~ s/T/U/g if $seqobj->alphabet eq 'rna'; # First we check if only the 'strict' letters are present in the
# sequence string If not, we check whether the remaining letters
# are ambiguous monomers or whether there are illegal letters in
# the string
# $alpha_array is a ref to an array of the 'strictly' allowed letters
my $alpha_array = $Alphabets_strict{$moltype} ; # $alphabet contains the allowed letters in string form
my $alphabet = join ('', @$alpha_array) ; unless ($seqstring =~ /[^$alphabet]/) { return 1 ; } # Next try to match with the alphabet's ambiguous letters
$alpha_array = $Alphabets{$moltype} ; $alphabet = join ('', @$alpha_array) ; unless ($seqstring =~ /[^$alphabet]/) { return 0 ; } # If we got here there is an illegal letter in the sequence
$seqobj->throw("Alphabet not OK for $seqobj");
}
_print_datadescriptionprevnextTop
sub _print_data {
    print "\n adenine  = :  $adenine\n ";
    print "\n guanine  = :  $guanine\n ";
    print "\n cytosine = :  $cytosine\n ";
    print "\n thymine  = :  $thymine\n ";
    print "\n uracil   = :  $uracil\n ";

    print "\n dna_A_wt = :  $dna_A_wt\n ";
    print "\n dna_C_wt = :  $dna_C_wt\n ";
    print "\n dna_G_wt = :  $dna_G_wt\n ";
    print "\n dna_T_wt = :  $dna_T_wt\n ";

    print "\n rna_A_wt = :  $rna_A_wt\n ";
    print "\n rna_C_wt = :  $rna_C_wt\n ";
    print "\n rna_G_wt = :  $rna_G_wt\n ";
    print "\n rna_U_wt = :  $rna_U_wt\n ";

    return 1;
}

1;
}
General documentation
DEVELOPERS NOTESTop
Ewan moved it from Bio::SeqStats to Bio::Tools::SeqStats
Heikki made tiny adjustments (+/- 0.01 daltons) to amino acid
molecular weights to have the output match values in SWISS-PROT.
Torsten added hydropathicity calculation.
FEEDBACKTop
Mailing ListsTop
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
  bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Support Top
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Reporting BugsTop
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted the web:
  https://redmine.open-bio.org/projects/bioperl/
AUTHOR - Peter SchattnerTop
Email schattner AT alum.mit.edu
CONTRIBUTOR - Torsten Seemann Top
Email torsten.seemann AT infotech.monash.edu.au
APPENDIXTop
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _