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Summary

Bio::Variation::Allele - Sequence object with allele-specific attributes

Package variables

Inherit

Bio::DBLinkContainerI Bio::PrimarySeq

Synopsis

  $allele1 = Bio::Variation::Allele->new ( -seq => 'A',
                                           -id  => 'AC00001.1',
                                           -alphabet => 'dna',
                                           -is_reference => 1
                                         );

Description

List of alleles describe known sequence alternatives in a variable region.
Alleles are contained in Bio::Variation::VariantI complying objects.
See Bio::Variation::VariantI for details.
Bio::Varation::Alleles are PrimarySeqI complying objects which can
contain database cross references as specified in
Bio::DBLinkContainerI interface, too.
A lot of the complexity with dealing with Allele objects are caused by
null alleles; Allele objects that have zero length sequence string.
In addition describing the allele by its sequence , it possible to
give describe repeat structure within the sequence. This done using
methods repeat_unit (e.g. 'ca') and repeat_count (e.g. 7).

Methods

Methods description

 Title   : is_reference
 Usage   : $obj->is_reference()
 Function: sets and returns boolean values. 
           Unset values return false.
 Example : $obj->is_reference()
 Returns : boolean
 Args    : optional true of false value

 Title   : add_DBLink
 Usage   : $self->add_DBLink($ref)
 Function: adds a link object
 Example :
 Returns : 
 Args    :

 Title   : each_DBLink
 Usage   : foreach $ref ( $self->each_DBlink() )
 Function: gets an array of DBlink of objects
 Example :
 Returns : 
 Args    :

 Title   : repeat_unit
 Usage   : $obj->repeat_unit('ca');
 Function: 

            Sets and returns the sequence of the repeat_unit the
            allele is composed of.

 Example : 
 Returns : string
 Args    : string

 Title   : repeat_count
 Usage   : $obj->repeat_count();
 Function: 

            Sets and returns the number of repeat units in the allele.

 Example : 
 Returns : string
 Args    : string

 Title   : count
 Usage   : $obj->count();
 Function: 

            Sets and returns the number of times this allele was observed.

 Example : 
 Returns : string
 Args    : string

 Title   : frequency
 Usage   : $obj->frequency();
 Function: 

            Sets and returns the frequency of the allele in the observed
            population.

 Example : 
 Returns : string
 Args    : string

Methods code

sub new {

    my($class, @args) = @_;
    my $self = $class->SUPER::new(@args);

    my($is_reference, $repeat_unit, $repeat_count) =
	   $self->_rearrange([qw(IS_REFERENCE
				 REPEAT_UNIT
				 REPEAT_COUNT
				 )],
			     @args);

    $is_reference && $self->is_reference($is_reference);
    $repeat_unit && $self->repeat_unit($repeat_unit);
    $repeat_count && $self->repeat_count($repeat_count);

    return $self; # success - we hope!

}

sub is_reference {

    my ($self,$value) = @_;
    if( defined $value) {
	$value ? ($value = 1) : ($value = 0);
	$self->{'is_reference'} = $value;
    }
    if( ! exists $self->{'is_reference'} ) {
	return 0;
    } 
    else {
	return $self->{'is_reference'};
    }

}

sub add_DBLink {

   my ($self,$com) = @_;
   if( ! $com->isa('Bio::Annotation::DBLink') ) {
       $self->throw("Is not a link object but a  [$com]");
   }
   push(@{$self->{'link'}},$com);

}

sub each_DBLink {

   my ($self) = @_;   
   return @{$self->{'link'}};

}

sub repeat_unit {

    my ($self,$value) = @_;
    if( defined $value) {
	$self->{'repeat_unit'} = $value;
    }
    if ($self->{'seq'} && $self->{'repeat_unit'} && $self->{'repeat_count'} ) {
	$self->warn("Repeats do not add up!") 
	    if ( $self->{'repeat_unit'} x $self->{'repeat_count'})  ne $self->{'seq'};
    }
    return $self->{'repeat_unit'};

}

sub repeat_count {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  not $value =~ /^\d+$/ ) {
	    $self->throw("[$value] for repeat_count has to be a positive integer\n");
	} else {
	    $self->{'repeat_count'} = $value;
	}
    }
    if ($self->{'seq'} && $self->{'repeat_unit'} && $self->{'repeat_count'} ) {
	$self->warn("Repeats do not add up!") 
	    if ( $self->{'repeat_unit'} x $self->{'repeat_count'})  ne $self->{'seq'};
    }
    return $self->{'repeat_count'};

}

sub count {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  not $value =~ /^\d+$/ ) {
	    $self->throw("[$value] for count has to be a positive integer\n");
	} else {
	    $self->{'count'} = $value;
	}
    }
    return $self->{'count'};

}

sub frequency {

    my ($self,$value) = @_;
    if( defined $value) {
	if (  not $value =~ /^\d+$/ ) {
	    $self->throw("[$value] for frequency has to be a positive integer\n");
	} else {
	    $self->{'frequency'} = $value;
	}
    }
    return $self->{'frequency'};
}


1;

}

General documentation

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The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _