Bio::Tools::Run::Alignment StandAloneFasta
SummaryIncluded librariesPackage variablesSynopsisDescriptionGeneral documentationMethods
Bio::Tools::Run::Alignment::StandAloneFasta - Object for the local
execution of the Fasta3 programs ((t)fasta3, (t)fastx3, (t)fasty3
Package variables
No package variables defined.
Included modules
Bio::Root::Root Bio::Tools::Run::WrapperBase
use Bio::Tools::Run::Alignment::StandAloneFasta;
use Bio::SeqIO;
use strict;
my @arg=(
'b' =>'15',
'O' =>'resultfile',
my $factory=Bio::Tools::Run::Alignment::StandAloneFasta->new(@arg); $factory->ktup(1); $factory->library('p'); #print result file name print $factory->O; my @fastreport=$factory->run($ARGV[0]); foreach (@fastreport) { print "Parsed fasta report:\n"; my $result = $_->next_result; while( my $hit = $result->next_hit()) { print "\thit name: ", $hit->name(), "\n"; while( my $hsp = $hit->next_hsp()) { print "E: ", $hsp->evalue(), "frac_identical: ", $hsp->frac_identical(), "\n"; } } } #pass in seq objects my $sio = Bio::SeqIO->new(-file=>$ARGV[0],-format=>"fasta"); my $seq = $sio->next_seq; my @fastreport=$factory->run($ARGV[0]);
This wrapper works with version 3 of the FASTA program package (see
W. R. Pearson and D. J. Lipman (1988), "Improved Tools for Biological
Sequence Analysis", PNAS 85:2444-2448 (Pearson and Lipman, 1988);
W. R. Pearson (1996) "Effective protein sequence comparison"
Meth. Enzymol. 266:227-258 (Pearson, 1996); Pearson et. al. (1997)
Genomics 46:24-36 (Zhang et al., 1997); Pearson, (1999) Meth. in
Molecular Biology 132:185-219 (Pearson, 1999). Version 3 of the FASTA
packages contains many programs for searching DNA and protein
databases and one program (prss3) for evaluating statistical
significance from randomly shuffled sequences.
Fasta is available at
No description
No description
Methods description
program_namecode    nextTop
 Title   : program_name
Usage : $factory->program_name()
Function: holds the program name
Returns: string
Args : None
 Title   : executable
Usage : my $exe = $blastfactory->executable('blastall');
Function: Finds the full path to the 'codeml' executable
Returns : string representing the full path to the exe
Args : [optional] name of executable to set path to
[optional] boolean flag whether or not warn when exe is not found
 Title   : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
 Title   : run
Usage : my @fasta_object = $factory->($input,$onefile); where $factory is the name of executable FASTA program; $input is file name containing the sequences in the format of fasta or Bio::Seq object or array of Bio::Seq object; $onefile is 0 if you want to save the outputs to different files default: outputs are saved in one file Function: Attempts to run an executable FASTA program and return array of fasta objects containing the fasta report Returns : aray of fasta report object If the user specify the output file(s), the raw fasta report will be saved Args : sequence object OR array reference of sequence objects filename of file containing fasta formatted sequences
 Title   : library
Usage : my $lb = $self->library
Function: Fetch or set the name of the library to search against
Returns : The name of the library
Args : No argument if user wants to fetch the name of library file;
A letter or a string of letter preceded by %;
(e.g. P or %pn, the letter is the character in the third field
of any line of fastlibs file ) or the name of library file
(if environmental variable FASTLIBS is not set);
if user wants to set the name of library file to search against
 Title   : output
Usage : $obj->output($newval)
Function: The output directory if we want to use this
Example :
Returns : value of output (a scalar)
Args : on set, new value (a scalar or undef, optional)
 Title   :  ktup
Usage : my $ktup = $self->ktup
Function: Fetch or set the ktup value for executable FASTA programs
Example :
Returns : The value of ktup if defined, else undef is returned
Args : No argument if user want to fetch ktup value; A integer value between 1-6 if user want to set the
ktup value
 Title   :  _setinput
Usage : Internal function, not to be called directly
Function: Create input file(s) for Blast executable
Example :
Returns : array of Bio::Seq object reference
Args : Seq object reference or input file name
 Title   : _exist
Usage : Internal function, not to be called directly
Function: Determine whether a executable FASTA program can be found
Cf. the DESCRIPTION section of this POD for how to make sure
for your FASTA installation to be found. This method checks for
existence of the blastall executable in the path.
Returns : 1 if FASTA program found at expected location, 0 otherwise.
Args : none
 Title   :  _setparams
Usage : Internal function, not to be called directly
Function: Create parameter inputs for FASTA executable
Returns : part of parameter string to be passed to FASTA program
Args : none
Methods code
  @FASTA_PARAMS=qw(a A b c E d f g h H i j l L M m n O o p Q q r R s S w x y z);
@OTHER_PARAMS =qw(program output database); foreach my $att (@FASTA_PARAMS, @OTHER_PARAMS) {$OK_FIELD{$att}++;} $ktup=2; %parameters=('H' => '', 'q' => '', 'm' =>'1', 'O' =>'');
sub new {
  my ($caller,@args)=@_;
  #chained new
my $self = $caller->SUPER::new(@args); while(@args){ my $attr = shift @args; my $value = shift @args; next if ($attr=~/^-/ || ! $attr); $self->$attr($value); } return $self;
    my $self = shift;
    my $attr = $AUTOLOAD;
    $attr =~ s/.*:://;
    $self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
    $self->{$attr} = shift if @_;
    return $self->{$attr};
sub program_name {
  my ($self) = shift;
  return $self->program(@_);
sub executable {
   my ($self, $exename, $exe,$warn) = @_;
   $exename = 'fasta34' unless defined $exename;

   if( defined $exe && -x $exe ) {
     $self->{'_pathtoexe'}->{$exename} = $exe;
   unless( defined $self->{'_pathtoexe'}->{$exename} ) {
       my $f = $self->program_path($exename);	    
       $exe = $self->{'_pathtoexe'}->{$exename} = $f if(-e $f && -x $f );
       #  This is how I meant to split up these conditionals --jason
# if exe is null we will execute this (handle the case where
# PROGRAMDIR pointed to something invalid)
unless( $exe ) { # we didn't find it in that last conditional
if( ($exe = $self->io->exists_exe($exename)) && -x $exe ) { $self->{'_pathtoexe'}->{$exename} = $exe; } else { $self->warn("Cannot find executable for $exename") if $warn; $self->{'_pathtoexe'}->{$exename} = undef; } } } return $self->{'_pathtoexe'}->{$exename};
sub program_dir {
  return Bio::Root::IO->catfile($ENV{FASTADIR}) if $ENV{FASTADIR};
sub run {
    my ($self,$input,$onefile)=@_;
    local * FASTARUN;

    my $program = $self->executable($self->program_name) ||  
	$self->throw("FASTA program not found or not executable.\n");
    # You should specify a library file
$self->throw("You didn't choose library.\n") unless ( $library); my @seqs = $self->_setinput($input); return 0 unless (@seqs); my @fastobj; my ($fhout, $tempoutfile)=$self->io->tempfile(-dir=>$self->tempdir); my $outfile=$self->O(); # The outputs from executable FASTA program will
# be saved into different files if $onefile is 0,
# else will be concatenated into one file
my $onfile = (!defined $onefile || $onefile =~ /^0$/); unless( $onfile ) { my $count=0; # do some fancy stuff here to test if we are running fasta34
# with mlib so we just pass in a single file rather than
# running fasta N times
# (not implemented yet)
foreach my $seq (@seqs){ $count++; # Decide if the output will be saved into a temporary file
if( $outfile ) { $self->O(sprintf("%s_%d",$outfile,$count)); } my ($fhinput,$teminputfile)= $self->io->tempfile(-dir=>$self->tempdir); my $temp = Bio::SeqIO->new(-fh=>$fhinput, '-format'=>'Fasta'); $temp->write_seq($seq); $temp->close(); close $fhinput; undef $fhinput; my $para= $self->_setparams; $para .=" $teminputfile $library $ktup"; $para ="$program $para"; my $object; unless( $outfile ) { open(FASTARUN, "$para |") || $self->throw($@); $object = Bio::SearchIO->new(-fh=>\*FASTARUN, -format=>"fasta"); } else { if ( $self->verbose() < 0) { $para .= ' >/dev/null 2>/dev/null'; } else { $self->debug("Going to execute: $para"); } my $status = system($para); $self->throw("$para crashed: $?\n" )unless ($status==0); $object = Bio::SearchIO->new(-file=>$self->O, -format=>"fasta"); } push @fastobj, $object; } } else { if ($outfile){ open (FILE, ">$outfile") or $self->throw("can't use $outfile:$!"); close(FILE); } foreach my $seq (@seqs){ my ($fhinput,$teminputfile)=$self->io->tempfile(-dir=>$self->tempdir); my $temp=Bio::SeqIO->new(-fh=>$fhinput, '-format'=>'fasta'); $temp->write_seq($seq); $temp->close(); close $fhinput; undef $fhinput; $self->O($tempoutfile) if( $outfile ); my $para= $self->_setparams; $para .= " $teminputfile $library $ktup"; $para ="$program $para"; my $object; unless( $outfile ) { open(FASTARUN, "$para |") || $self->throw($@); $object=Bio::SearchIO->new(-fh=>\*FASTARUN, -format=>"fasta"); } else { if ( $self->verbose() < 0) { $para .= ' >/dev/null 2>/dev/null'; } else { $self->debug("Going to execute: $para"); } my $status = system($para); $self->throw("$para crashed: $?\n" )unless ($status==0); $object = Bio::SearchIO->new(-file=>$self->O, -format=>"fasta"); } push @fastobj, $object; # The output in the temporary file
# will be saved at the end of $outfile
if($outfile){ open (FHOUT, $tempoutfile) or die("can't open the $tempoutfile file"); open (FH, ">>$outfile") or die("can't use the $outfile file"); print FH (<FHOUT>); close (FHOUT); close (FH); } } } return @fastobj;
sub library {
    return $library if (!defined($lb));

    if ( ($lb =~ /^%[a-zA-Z]+$/)||($lb=~ /^[a-zA-Z]$/)){
	if(! defined $ENV{'FASTLIBS'} ){
	    $self->throw("abbrv. list request but FASTLIBS undefined, cannot use $lb");
    } else {
	unless ( -e $lb){
	    $self->throw("cannot open $lb library");

    return $library=$lb;

*database =\& library;
sub output {
    my $self = shift;

    return $self->{'output'} = shift if @_;
    return $self->{'output'};
sub ktup {
  if(!defined($k)){return $ktup;}
  if ($k =~ /^[1-6]$/){
    return $ktup
  } else {
    $self->warn("You should set the ktup value between 1-6. The FASTA program will decide your default ktup value.");
    return $ktup= undef;
sub _setinput {
    my ($self, $input) = @_;

    if( ! defined $input ) { 
	$self->throw("Calling fasta program with no input");  

    my @seqs;
    if( ! ref $input ) {
	if( -e $input ) {
	    my $seqio = Bio::SeqIO->new(-format => 'fasta', -file => $input);
	    while( my $seq = $seqio->next_seq ) {
		push @seqs, $seq;
	} else {
	    $self->throw("Input $input was not a valid filename");
    } elsif( ref($input) =~ /ARRAY/i ) {
	foreach ( @$input ) {
	    if( ref($_) && $_->isa('Bio::PrimarySeqI') ) {
		push @seqs, $_;
	    } else {
		$self->warn("Trying to add a " . ref($_) ." but expected a Bio::PrimarySeqI");
	if( ! @seqs) {
	    $self->throw("Did not pass in valid input -- no sequence objects found");
    } elsif( $input->isa('Bio::PrimarySeqI') ) {
	push @seqs, $input;
    return @seqs;
sub _exist {
  my  $exe = shift @_;

  return 0 unless($exe =~ /fast|ssearch/);

  $exe .='.exe' if ($^O =~ /mswin/i);

  my $f;
  return ($f=Bio::Root::IO->exists_exe($exe))&&(-x $f);
sub _setparams {
    my ($self,$attr,$value);
    $self = shift;
    my $para = "";
    foreach my $attr(@FASTA_PARAMS) {
	$value = $self->$attr();
	next unless (defined $value);
	$para .=" -$attr $value";
    $para .= " -q ";
    return $para;

General documentation
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AUTHOR - Tiequan ZhangTop
       Adapted for bioperl by Shawn Hoon
Enhanced by Jason Stajich
The rest of the documendation details each of the object
methods. Internal methods are preceeded with a underscore