Bio::Tools::Run
Hmmer
Toolbar
Summary
Bio::Tools::Run::Hmmer - Wrapper for local execution of hmmalign, hmmbuild,
hmmcalibrate, hmmemit, hmmpfam, hmmsearch
Package variables
No package variables defined.
Included modules
Bio::AlignIO
Bio::SearchIO
Bio::SeqIO
Inherit
Bio::Tools::Run::WrapperBase
Synopsis
# run hmmsearch (similar for hmmpfam)
my $factory = Bio::Tools::Run::Hmmer->new(-hmm => 'model.hmm');
# Pass the factory a Bio::Seq object or a file name, returns a Bio::SearchIO
my $searchio = $factory->hmmsearch($seq);
while (my $result = $searchio->next_result){
while(my $hit = $result->next_hit){
while (my $hsp = $hit->next_hsp){
print join("\t", ( $result->query_name,
$hsp->query->start,
$hsp->query->end,
$hit->name,
$hsp->hit->start,
$hsp->hit->end,
$hsp->score,
$hsp->evalue,
$hsp->seq_str,
)), "\n";
}
}
}
# build a hmm using hmmbuild
my $aio = Bio::AlignIO->new(-file => "protein.msf", -format => 'msf');
my $aln = $aio->next_aln;
my $factory = Bio::Tools::Run::Hmmer->new(-hmm => 'model.hmm');
$factory->hmmbuild($aln);
# calibrate the hmm
$factory->calibrate();
# emit a sequence stream from the hmm
my $seqio = $factory->hmmemit();
# align sequences to the hmm
my $alnio = $factory->hmmalign(@seqs);
Description
Wrapper module for Sean Eddy's HMMER suite of program to allow running of
hmmalign, hmmbuild, hmmcalibrate, hmmemit, hmmpfam and hmmsearch. Binaries are
available at
http://hmmer.janelia.org/
You can pass most options understood by the command-line programs to new(), or
set the options by calling methods with the same name as the argument. In both
instances, case sensitivity matters.
Additional methods are hmm() to specifiy the hmm file (needed for all HMMER
programs) which you would normally set in the call to new().
The HMMER programs must either be in your path, or you must set the environment
variable HMMERDIR to point to their location.
Methods
Methods description
Title : new
Usage : $HMMER->new(@params)
Function: Creates a new HMMER factory
Returns : Bio::Tools::Run::HMMER
Args : -hmm => filename # the hmm, used by all program types; if not set
# here, must be set with hmm() method prior to
# running anything
-_READMETHOD => 'hmmer' (default) || 'hmmer_pull' # the parsing
# module to use for
# hmmpfam/hmmsearch
Any option supported by a Hmmer program, where switches are given
a true value, eg. -q => 1, EXCEPT for the following which are handled
internally/ incompatible: h verbose a compat pvm
WARNING: the default sequence format passed to hmmpfam is msf. If
you are using a different format, you need to pass it with informat.
e.g.
my $factory = Bio::Tools::Run::Hmmer->new(-hmm => 'model.hmm',
-informat => 'fasta');
-q is synonymous with -quiet
-o is synonymous with -outfile
# may be specified here, allowing run() to be used, or
# it can be ommitted and the corresponding method (eg.
# hmmalign()) used later.
-program => hmmalign|hmmbuild|hmmcalibrate|hmmemit|hmmpfam|hmmsearch |
Title : run
Usage : $obj->run($seqFile)
Function: Runs one of the Hmmer programs, according to the current setting of
program() (as typically set during new(-program => 'name')).
Returns : A Bio::SearchIO, Bio::AlignIO, Bio::SeqIO or boolean depending on
the program being run (see method corresponding to program name for
details).
Args : A Bio::PrimarySeqI, Bio::Align::AlignI or filename |
Title : hmmalign
Usage : $obj->hmmalign()
Function: Runs hmmalign
Returns : A Bio::AlignIO
Args : list of Bio::SeqI OR Bio::Align::AlignI OR filename of file with
sequences or an alignment |
Title : hmmbuild
Usage : $obj->hmmbuild()
Function: Runs hmmbuild, outputting an hmm to the file currently set by method
hmm() or db(), or failing that, o() or outfile(), or failing that, to
a temp location.
Returns : true on success
Args : Bio::Align::AlignI OR filename of file with an alignment |
Title : hmmcalibrate
Usage : $obj->hmmcalibrate()
Function: Runs hmmcalibrate
Returns : true on success
Args : none (hmm() must be set, most likely by the -hmm option of new()), OR
optionally supply an hmm filename to set hmm() and run |
Title : hmmemit
Usage : $obj->hmmemit()
Function: Runs hmmemit
Returns : A Bio::SeqIO
Args : none (hmm() must be set, most likely by the -hmm option of new()), OR
optionally supply an hmm filename to set hmm() and run |
Title : hmmpfam
Usage : $obj->hmmpfam()
Function: Runs hmmpfam
Returns : A Bio::SearchIO
Args : A Bio::PrimarySeqI, Bio::Align::AlignI or filename |
Title : hmmsearch
Usage : $obj->hmmsearch()
Function: Runs hmmsearch
Returns : A Bio::SearchIO
Args : A Bio::PrimarySeqI, Bio::Align::AlignI or filename |
Title : _setinput
Usage : $obj->_setinput()
Function: Internal(not to be used directly)
Returns : filename
Args : A Bio::PrimarySeqI, Bio::Align::AlignI or filename |
Title : _run
Usage : $obj->_run()
Function: Internal(not to be used directly)
Returns : Bio::SearchIO
Args : file name |
Title : _setparams
Usage : Internal function, not to be called directly
Function: creates a string of params to be used in the command string
Returns : string of params
Args : none |
Title : program_name
Usage : $factory>program_name()
Function: holds the program name
Returns : string
Args : none |
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns : string
Args : none |
Title : _writeSeqFile
Usage : obj->_writeSeqFile($seq)
Function: Internal(not to be used directly)
Returns : filename
Args : list of Bio::SeqI |
Title : _writeAlignFile
Usage : obj->_writeAlignFile($seq)
Function: Internal(not to be used directly)
Returns : filename
Args : list of Bio::Align::AlignI |
Methods code
sub new
{ my($class, @args) = @_;
my $self = $class->SUPER::new(@args);
$self->_set_from_args(\@args, -methods => {(map { $_ => $ALL{$_} } keys %ALL),
(map { $_ => $OTHER{$_} } keys %OTHER),
(map { $_ => $_ }
(@ALIGN_PARAMS,
@ALIGN_SWITCHES,
@BUILD_PARAMS,
@BUILD_SWITCHES,
@CALIBRATE_PARAMS,
@CALIBRATE_SWITCHES,
@EMIT_PARAMS,
@EMIT_SWITCHES,
@PFAM_PARAMS,
@PFAM_SWITCHES,
@SEARCH_PARAMS,
@SEARCH_SWITCHES))},
-create => 1,
-case_sensitive => 1);
$self->informat || $self->informat($DefaultFormat);
$self->_READMETHOD || $self->_READMETHOD($DefaultReadMethod);
return $self;} | sub run
{ my $self = shift;
my $program = lc($self->program_name || $self->throw("The program must already be specified"));
$self->can($program) || $self->throw("'$program' wasn't a valid program");
return $self->$program(@_);} | sub hmmalign
{ my $self = shift;
$self->program_name('hmmalign');
my $input = $self->_setinput(@_);
unless (defined $self->o()) {
$self->q(1);
}
if (! $self->outformat) {
$self->outformat($DefaultFormat);
}
return $self->_run($input);} | sub hmmbuild
{ my $self = shift;
$self->program_name('hmmbuild');
my $input = $self->_setinput(@_);
unless (defined $self->hmm()) {
$self->hmm($self->o() || $self->io->tempfile(-dir => $self->tempdir));
}
return $self->_run($input);} | sub hmmcalibrate
{ my ($self, $hmm) = @_;
$self->program_name('hmmcalibrate');
$self->hmm($hmm) if $hmm;
$self->hmm || $self->throw("hmm() must be set first");
return $self->_run();} | sub hmmemit
{ my ($self, $hmm) = @_;
$self->program_name('hmmemit');
$self->hmm($hmm) if $hmm;
$self->hmm || $self->throw("hmm() must be set first");
unless (defined $self->o()) {
$self->q(1);
}
return $self->_run();} | sub hmmpfam
{ my $self = shift;
$self->program_name('hmmpfam');
my $input = $self->_setinput(@_);
return $self->_run($input);} | sub hmmsearch
{ my $self = shift;
$self->program_name('hmmsearch');
my $input = $self->_setinput(@_);
return $self->_run($input);} | sub _setinput
{ my ($self, @things) = @_;
@things || $self->throw("At least one input is required");
my $infile;
if (ref $things[0] && $things[0]->isa("Bio::PrimarySeqI") ){
$infile = $self->_writeSeqFile(@things);
}
elsif(ref $things[0] && $things[0]->isa("Bio::Align::AlignI")){
$infile = $self->_writeAlignFile(@things);
}
elsif (-e $things[0]) {
$infile = $things[0];
}
else {
$self->throw("Unknown kind of input '@things'");
}
return $infile;} | sub _run
{ my ($self, $file) = @_;
my $str = $self->executable;
$str .= $self->_setparams;
$str .= ' '.$file if $file;
$self->debug("HMMER command = $str");
my $progname = $self->program_name;
my @in;
my @verbose = (-verbose => $self->verbose);
if ($progname =~ /align|build|emit/) {
my $outfile = $self->o;
if ($outfile || $progname eq 'hmmbuild') {
$str .= " > /dev/null" if $self->quiet;
if ($progname eq 'hmmbuild') {
my $status = system($str);
return $status ? 0 : 1;
}
else {
system($str) && $self->throw("HMMER call ($str) crashed: $?\n");
@in = (-file => $outfile);
}
}
else {
open(my $fh, "$str |") || $self->throw("HMMER call ($str) crashed: $?\n");
@in = (-fh => $fh);
}
}
elsif ($progname =~ /pfam|search/i) {
open(my $fh, "$str |") || $self->throw("HMMER call ($str) crashed: $?\n");
return Bio::SearchIO->new(-fh => $fh,
@verbose,
-format => $self->_READMETHOD);
}
if ($progname eq 'hmmalign') {
return Bio::AlignIO->new(@in,
@verbose,
-format => $self->outformat);
}
elsif ($progname eq 'hmmemit') {
return Bio::SeqIO->new(@in,
@verbose,
-format => 'fasta');
}
elsif ($progname =~ /calibrate/) {
$str .= " > /dev/null 2> /dev/null" if $self->quiet;
my $status = system($str);
return $status ? 0 : 1;
}} | sub _setparams
{ my $self = shift;
my @execparams;
my @execswitches;
SWITCH: for ($self->program_name) {
/align/ && do { @execparams = @ALIGN_PARAMS;
@execswitches = @ALIGN_SWITCHES;
last SWITCH; };
/build/ && do { @execparams = @BUILD_PARAMS;
@execswitches = @BUILD_SWITCHES;
last SWITCH; };
/calibrate/ && do { @execparams = @CALIBRATE_PARAMS;
@execswitches = @CALIBRATE_SWITCHES;
last SWITCH; };
/emit/ && do { @execparams = @EMIT_PARAMS;
@execswitches = @EMIT_SWITCHES;
last SWITCH; };
/pfam/ && do { @execparams = @PFAM_PARAMS;
@execswitches = @PFAM_SWITCHES;
last SWITCH; };
/search/ && do { @execparams = @SEARCH_PARAMS;
@execswitches = @SEARCH_SWITCHES;
last SWITCH; };
}
my $param_string = $self->SUPER::_setparams(-params =>\@ execparams,
-switches =>\@ execswitches,
-mixed_dash => 1);
my $hmm = $self->hmm || $self->throw("Need to specify either HMM file or Database");
$param_string .= ' '.$hmm;
return $param_string;} | sub program_name
{ my $self = shift;
if (@_) {
$self->{program_name} = shift;
delete $self->{'_pathtoexe'};
}
return $self->{program_name} || '';} | sub program_dir
{ return $ENV{HMMERDIR} if $ENV{HMMERDIR};} | sub _writeSeqFile
{ my ($self, @seq) = @_;
my ($tfh, $inputfile) = $self->io->tempfile(-dir=>$self->tempdir);
$self->informat('fasta');
my $out = Bio::SeqIO->new(-fh => $tfh , '-format' => 'fasta');
foreach my $s (@seq) {
$out->write_seq($s);
}
$out->close();
$out = undef;
close($tfh);
undef $tfh;
return $inputfile;} | sub _writeAlignFile
{ my ($self, @align) = @_;
my ($tfh, $inputfile) = $self->io->tempfile(-dir=>$self->tempdir);
my $out = Bio::AlignIO->new('-fh' => $tfh, '-format' => $self->informat);
foreach my $a (@align) {
$out->write_aln($a);
}
$out->close();
$out = undef;
close($tfh);
undef $tfh;
return $inputfile;
}
1;} | General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
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bioperl-l@bioperl.org - General discussion
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Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
Shawn Hoon shawnh-at-gmx.net
Jason Stajich jason -at- bioperl -dot- org
Scott Markel scott -at- scitegic -dot com
Sendu Bala bix@sendu.me.uk
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _