Bio::Tools::Run
Profile
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Summary
Bio::Tools::Run::Profile
Package variables
No package variables defined.
Included modules
Bio::Factory::ApplicationFactoryI
Bio::Root::IO
Bio::Root::Root
Bio::SeqIO
Bio::Tools::Profile
Bio::Tools::Run::WrapperBase
Inherit
Bio::Root::Root Bio::Tools::Run::WrapperBase
Synopsis
Build a Profile factory
# $paramfile is the full path to the seg binary file
my @params = ('DB',$dbfile,'PROGRAM',$paramfile);
my $factory = Bio::Tools::Run::Profile->new($param);
# Pass the factory a Bio::PrimarySeqI object
# @feats is an array of Bio::SeqFeature::Generic objects
my @feats = $factory->run($seq);
Description
Wrapper module for the pfscan program
Methods
Methods description
Title : program_name Usage : $factory>program_name() Function: holds the program name Returns: string Args : None |
Title : program_dir Usage : $factory->program_dir(@params) Function: returns the program directory, obtained from ENV variable. Returns: string Args : |
Title : new Usage : my $factory= Bio::Tools::Run::Profile->new($param); Function: creates a new Profile factory Returns: Bio::Tools::Run::Profile Args : |
Title : predict_protein_features Usage : DEPRECATED. Use $factory->run($seq) instead. Function: Runs Profile and creates an array of featrues Returns : An array of Bio::SeqFeature::FeaturePair objects Args : A Bio::PrimarySeqI |
Title : run Usage : my @feats = $factory->run($seq) Function: Runs Profile Returns : An array of Bio::SeqFeature::FeaturePair objects Args : A Bio::PrimarySeqI |
Title : _run Usage : $factory->_run() Function: Makes a system call and runs pfscan Returns : An array of Bio::SeqFeature::FeaturePair objects Args : |
Title : _writeSeqFile Usage : $factory->_writeSeqFile($seq) Function: Creates a file from the given seq object Returns : A string(filename) Args : Bio::PrimarySeqI |
Methods code
BEGIN { @PROFILE_PARAMS=qw(DB PROGRAM VERBOSE);
foreach my $attr ( @PROFILE_PARAMS)
{ $OK_FIELD{$attr}++;} |
sub program_name
{ return 'pfscan'; } |
sub program_dir
{ return Bio::Root::IO->catfile($ENV{PROFILEDIR}) if $ENV{PROFILEDIR};} |
sub AUTOLOAD
{ my $self = shift;
my $attr = $AUTOLOAD;
$attr =~ s/.*:://;
$attr = uc $attr;
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};} |
sub new
{ my ($class,@args) = @_;
my $self = $class->SUPER::new(@args);
$self->io->_initialize_io();
my ($attr, $value);
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/ ); $self->$attr($value);
}
return $self;} |
sub predict_protein_features
{ return shift->run(@_); } |
sub run
{ my ($self,$seq) = @_;
my @feats;
if (ref($seq) ) {
if (ref($seq) =~ /GLOB/) {
$self->throw("cannot use filehandle");
}
my $display_id = $seq->display_id;
my $infile1 = $self->_writeSeqFile($seq);
$self->_input($infile1);
@feats = $self->_run($display_id);
unlink $infile1;
}
else {
$self->_input($seq);
@feats = $self->_run();
}
return @feats;} |
sub _input()
{ my ($self,$infile1) = @_;
if(defined $infile1){
$self->{'input'}=$infile1;
}
return $self->{'input'};} |
sub _run
{ my ($self,$display_id)= @_;
my ($tfh,$outfile) = $self->io->tempfile(-dir=>$self->tempdir());
close($tfh);
undef $tfh;
my $str =$self->executable.' -fz '.$self->_input." ".$self->DB." > ".$outfile;
my $status = system($str);
$self->throw( "Profile call ($str) crashed: $?\n ") unless $status==0;
my $filehandle;
if (ref ($outfile) !~ /GLOB/) {
open (PROFILE, "<".$outfile) or $self->throw ("Couldn't open file ".$outfile.": $!\n");
$filehandle =\* PROFILE;
}
else {
$filehandle = $outfile;
}
my $profile_parser = Bio::Tools::Profile->new(-fh=>$filehandle);
my @profile_feat;
while(my $profile_feat = $profile_parser->next_result){
$profile_feat->seq_id($display_id);
push @profile_feat, $profile_feat;
}
$self->cleanup();
unlink $outfile;
return @profile_feat;} |
sub _writeSeqFile
{ my ($self,$seq) = @_;
my ($tfh,$inputfile) = $self->io->tempfile(-dir=>$self->tempdir());
my $in = Bio::SeqIO->new(-fh => $tfh , '-format' => 'fasta');
$in->write_seq($seq);
$in->close();
undef $in;
close($tfh);
undef $tfh;
return $inputfile;
}
1;} |
General documentation
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
| AUTHOR - Balamurugan Kumarasamy | Top |
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
Title : _input
Usage : $factory->_input($seqFile)
Function: get/set for input file
Returns :
Args :